A5100607601- Chromosomal and Genetic Variations
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- RNA Research and Splicing
- Crop Yield and Soil Fertility
- RNA modifications and cancer
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Food composition and properties
- Plant Genetic and Mutation Studies
- RNA Interference and Gene Delivery
- Genetic Mapping and Diversity in Plants and Animals
- Plant Virus Research Studies
- Genetic and Environmental Crop Studies
- Rice Cultivation and Yield Improvement
- Plant Pathogens and Fungal Diseases
- Business and Management Studies
- Plant responses to elevated CO2
- Agricultural pest management studies
- Plant Pathogens and Resistance
- Brain Tumor Detection and Classification
University of Lisbon
2011-2025
University of Guelph
2025
Universidade Federal de Pernambuco
2025
University of Missouri
2011
Universidade Nova de Lisboa
2004
University of Washington
2004
Instituto Superior de Gestão
1997
Allopolyploidy is a significant evolutionary process, resulting in new species with diploid or greater chromosome complements derived from two more progenitor species. We examined the chromosomal consequences of genomic merger Arabidopsis suecica , allotetraploid hybrid thaliana and arenosa . Fluorescence situ hybridization centromere, nucleolus organizer region (NOR), 5S rRNA gene probes reveals expected numbers chromosomes natural A but one pair NORs -derived loci are missing. Similarly,...
Nucleolar dominance describes the silencing of one parental set ribosomal RNA (rRNA) genes in a genetic hybrid, an epigenetic phenomenon that occurs on scale second only to X-chromosome inactivation mammals. An interference (RNAi) knockdown screen revealed predicted Arabidopsis histone deacetylase, HDA6, is required for rRNA gene nucleolar dominance. In vivo, derepression silenced upon HDA6 accompanied by nucleolus organizer region (NOR) decondensation, loss promoter cytosine methylation,...
Nucleolar dominance is an epigenetic phenomenon in plant and animal genetic hybrids that describes the expression of 45S ribosomal RNA genes (rRNA genes) inherited from only one progenitor due to silencing other progenitor's rRNA genes. are tandemly arrayed at nucleolus organizer regions (NORs) span millions basepairs, thus gene nucleolar occurs on a scale second X-chromosome inactivation female mammals. In Arabidopsis suecica, allotetraploid hybrid A. thaliana arenosa, -derived subjected...
BackgroundPolyploidization is a major evolutionary process in plants where hybridization and chromosome doubling induce enormous genomic stress can generate genetic epigenetic modifications. However, proper evaluation of DNA sequence restructuring events the precise characterization sequences involved are still sparse.Methodology/Principal FindingsInter Retrotransposons Amplified Polymorphism (IRAP), Microsatellite (REMAP) Inter Simple Sequence Repeat (ISSR) largely confirmed absence any...
In genetic hybrids, nucleolus formation on chromosomes inherited from only one parent is the epigenetic phenomenon, nucleolar dominance. By using Arabidopsis suecica , allotetraploid hybrid of thaliana and arenosa natural variation in dominance was found to occur, providing a unique opportunity examine homologous organizer regions (NORs) their active inactive states. A. strain LC1, NORs derived are active, whereas -derived silenced. 9502, both parental species active. When partially, but not...
Faba bean (Vicia faba L.) is a facultative cross-pollinating legume crop with great importance for food and feed due to its high protein content as well the important role in soil fertility nitrogen fixation. In this work we evaluated genetic diversity population structure of accessions from Western Mediterranean basin wild related species. For that purpose screened 53 V. faba, 2 johannis 7 narbonensis Portugal, Spain Morocco 28 Single Sequence Repeats (SSR). SSR genotyping showed number...
Wheat is one of the most important cereals for food and feed, it is, therefore, necessary to determine effects short-term high temperature events (heatwaves) during grain filling. These heatwave are increasingly common, especially in Portugal. In this work, seven commercial varieties recommended production Portugal were submitted one-week (HT) treatment ten days after anthesis evaluate heat on yield quality. Grain parameters, such as number weight, evaluated well composition through...
Processing computer vision applications (CVA) on mobile devices is challenging due to limited battery life and computing power. While cloud-based remote processing of CVA offers abundant computational resources, it introduces latency issues that can hinder real-time applications. To overcome this problem, offloading edge servers has been adopted by industry academic research. Furthermore, 5G access also benefit with lower higher bandwidth than previous cellular generations. As the number...
Introduction Durum wheat is the most important cereal in Mediterranean regions, where drought negatively affects grain yield. Therefore, our objective was to perform a multi-omics and integration analysis conjunction with physiological trials improve understanding of tolerance mechanisms durum wheat. Methods Genome-wide association study (GWAS) for yield components performed on panel 225 elite genotypes evaluated eight sites under irrigated rainfed conditions. Based parameters (net...
Background Chromatin organizational and topological plasticity, its functions in gene expression regulation, have been strongly revealed by the analysis of nucleolar dominance hybrids polyploids where one parental set ribosomal RNA (rDNA) genes that are clustered organizing regions (NORs), is rendered silent epigenetic pathways heterochromatization. However, information on behaviour dominant NORs very sparse needed for an integrative knowledge differential transcription levels chromatin...
Genomic diversity of Portuguese accessions Avena species—diploid A. strigosa and hexaploids sativa sterilis—was evaluated through molecular cytological analysis 45S rDNA, other repetitive sequences previously studied in cereal species—rye subtelomeric sequence (pSc200) centromeric (CCS1). Additionally, retrotransposons microsatellites targeting methodologies—IRAP (inter-retrotransposon amplified polymorphism) REMAP (retrotransposon-microsatellite polymorphism)—were performed. A very high...
Faba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba that cultivated some regions the country was evaluated using Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It shown molecular markers based retrotransposons previously identified other species can be efficiently used variability assessment Vicia faba. IRAP experiment targeting Athila yielded most informative...
Wheat is undoubtedly one of the most important crops worldwide and it essential to study how distinct varieties answer heat waves associated with climatic changes, in order design adequate wheat breeding strategies. To assess high temperature (HT) impact grain characteristics, seven commercial varieties, which have been recommended for production Portugal, were submitted one-week HT treatment ten days after anthesis. Firstly, predicted technological quality was determined by giving scores...
Nucleolar dominance is an enigma. The puzzle of differential amphiplasty has remained unresolved since it was first recognised and described in Crepis hybrids by Navashin 1934. Here we review the body knowledge that grown out many models have tried to find genetic basis for rRNA gene expression hybrids, present a new interpretation. We propose discuss chromatin imprinting model which re-interprets terms two genomes differing size occupying common space within nucleus, with heterochromatin as...
The plant stress response has been extensively characterized at the biochemical and physiological levels. However, knowledge concerning repetitive sequence genome fraction modulation during extreme temperature conditions is scarce. We studied high-temperature effects on subtelomeric sequences (pSc200) 45S rDNA in rye seedlings submitted to 40°C 4 h. Chromatin organization patterns were evaluated through fluorescent situ hybridization transcription levels assessed using quantitative real-time...
The genus Avena comprises four distinct genomes organized in diploid (AA or CC), tetraploid (AABB AACC) and hexaploid species (AACCDD), constituting an interesting model for phylogenetic analysis. aim of this work was to characterize 45S rDNA intergenic spacer (IGS) variability representative genome diversity-A. strigosa (AA), A. ventricosa (CvCv), eriantha (CpCp), barbata (AABB), murphyi (AACC), sativa (AACCDD) sterilis through the assessment 5' external transcribed (5'-ETS), a promising...