A5025953526- Circadian rhythm and melatonin
- Birth, Development, and Health
- MicroRNA in disease regulation
- Adipokines, Inflammation, and Metabolic Diseases
- Stress Responses and Cortisol
- Extracellular vesicles in disease
- Spaceflight effects on biology
- Neuroendocrine regulation and behavior
- Pregnancy and preeclampsia studies
- Mechanisms of cancer metastasis
- Cancer, Lipids, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Cancer-related molecular mechanisms research
- Psychological and Temporal Perspectives Research
- COVID-19 Impact on Reproduction
- Congenital heart defects research
- interferon and immune responses
- Regulation of Appetite and Obesity
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- Reproductive System and Pregnancy
- Hormonal Regulation and Hypertension
Universidad de Los Andes, Chile
2020-2024
Universidad Mayor
2022
Universidad Autónoma de Chile
2019
Pontificia Universidad Católica de Chile
2015-2018
Austral University of Chile
2007-2014
University of Chile
2009-2011
Surprisingly, in our modern 24/7 society, there is scant information on the impact of developmental chronodisruption like one experienced by shift worker pregnant women fetal and postnatal physiology. There are important differences between maternal circadian systems; for instance, suprachiasmatic nucleus master clock mother but not fetus. Despite this, several tissues/organs display oscillations Our hypothesis that plasma melatonin rhythm drives system, which turn relies this to other...
The adrenal gland in the adult is a peripheral circadian clock involved coordination of energy intake and expenditure, required for adaptation to external environment. During fetal life, present nonhuman primate gland. Whether this extends like rat unknown. Here we explored vivo vitro whether entrained by melatonin. We measured 24-h changes content corticosterone expression genes Per-2 Bmal-1 steroidogenic acute regulatory protein (StAR), Mt1 melatonin receptor, early growth response 1...
// Sumie Kato 1 , Lorena Abarzua-Catalan César Trigo Ana Delpiano Cristobal Sanhueza 2 Karen García Carolina Ibañez Katherine Hormazábal Daniela Diaz Jorge Brañes Enrique Castellón 4 Erasmo Bravo 5 Gareth Owen 3 and Mauricio A. Cuello Division of Obstetrics Gynecology, Faculty Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile Department Hematology Oncology, Physiological Sciences, Biological University Gynecologic Oncology...
Epidemiological and experimental evidence correlates adverse intrauterine conditions with the onset of disease later in life. For a fetus to achieve successful transition extrauterine life, myriad temporally integrated humoral/biophysical signals must be accurately provided by mother. We others have shown existence daily rhythms fetus, peripheral clocks being entrained maternal cues, such as transplacental melatonin signaling. Among developing tissues, fetal hippocampus is key structure for...
In the last few decades, it has been established that astrocytes play key roles in regulation of neuronal morphology. However, contribution astrocyte-derived small extracellular vesicles (sEVs) to morphological differentiation neurons only recently addressed. Here, we showed cultured expressing a GFP-tagged version stress-regulated astrocytic enzyme Aldolase C (Aldo C-GFP) release are transferred into hippocampal neurons. Surprisingly, Aldo C-GFP-containing sEVs C-GFP sEVs) displayed an...
We previously demonstrated that melatonin is involved in the regulation of adrenal glucocorticoid production diurnal primates through activation MT1 membrane-bound receptors. However, whether has a similar role nocturnal rodents remains unclear. Using an integrative approach, here we show adult rat gland expresses functional receptor rhythmic fashion. found that: 1) expression cognate mRNA encoding for receptor, displaying higher levels day/night transition (1800-2200 h); 2) predicted 37-kDa...
In nonhuman primates and rodents, melatonin acting directly on the adrenal gland, inhibits glucocorticoid response to ACTH. these species, an intrinsic circadian clock is involved in ACTH-stimulated production. We investigated whether findings apply human gland by determining i) expression of genes vivo ii) direct effects explants over a) PER1 (Period 1) mRNA BMAL1 [Brain-Muscle (ARNT)-like] protein, ACTH-induced steroidogenic acute regulatory protein (StAR), 3β-hydroxysteroid dehydrogenase...
In the capuchin monkey (Cebus apella), a new-world nonhuman primate, maternal exposure to constant light during last third of gestation induces precocious maturation fetal adrenal and increased plasma cortisol in newborn. Here, we further explored effects this challenge on developmental programming function newborn infant monkeys. We measured (i) dehydroepiandrosterone sulphate (DHAS) response ACTH infants with suppressed endogenous ACTH, (ii) DHAS vitro, (iii) weight expression level key...
Timely production of glucocorticoid hormones in response to ACTH is essential for survival by coordinating energy intake and expenditure acting as homeostatic regulators against stress. Adrenal cortisol clock time dependent, suggesting that an intrinsic circadian oscillator the adrenal cortex contributes modulate ACTH. Circadian gene expression has been reported several species. However, there are no reports accounting potential involvement proteins on Here we explored whether protein...
Fluoxetine is a selective serotonin reuptake inhibitor (SSRI) used to treat mood and anxiety disorders. Chronic treatment with this antidepressant drug thought favor functional recovery by promoting structural molecular changes in several forebrain areas. At the synaptic level, chronic fluoxetine induces an increased size density of dendritic spines ratio GluN2A over GluN2B N-methyl-D-aspartate (NMDA) receptor subunits. The "maturation"-promoting observed after should also induce remodeling...
Alzheimer's disease (AD) is the most prevalent age-associated neurodegenerative disease. A decrease in autophagy during aging contributes to brain disorders by accumulating potentially toxic substrates neurons. Rubicon a well-established inhibitor of all cells. However, participates different pathways depending on cell type, and little information currently available neuronal Rubicon's role AD context. Here, we investigated cell-specific expression postmortem samples from patients 5xFAD mice...
Chronic stress can trigger several pathologies including mood disorders for which no clear diagnostic molecular markers have been established yet. Attractive biomarker sources are extracellular vesicles (EVs). Evs released by cells in health and disease contain genetic material, proteins lipids characteristic of the cell state. Here we show that recovered from blood animals exposed to a repeated interrupted protocol (RIS) different protein profile compared those obtained control animals....
Abstract Introduction: High-grade serous ovarian cancer remains as the most lethal gynecologic cancer. Despite achieving optimal debunking using ultra-radical surgery followed by platinum-based chemotherapy, in long term, of patient will die from their disease, mainly due to extensive carcinomatosis, bowel obstruction and cachexia. Recently our group has provided biological evidence supporting role obesity impairing prognosis. Obesity is considered a chronic low-grade inflammation state...
Abstract Introduction: The current knowledge in ovarian epithelial carcinogenesis grants a major role for chronic inflammation driving the multiple steps natural history of most deadly gynecological cancer. Different environmental stimuli have been associated with an increased cancer risk. Growing evidence has become to support potential obesity as one conditioning factors. Leptin, adipokine, controlling metabolism and energy expenditure normally circulating at higher serum levels among...