Aims: Cells have developed quality control systems for protection against proteotoxicity. Misfolded and aggregation-prone proteins, which are behind the initiation progression of many neurodegenerative diseases (ND), known to challenge proteostasis network cells. We aimed explore role DNJ-27/ERdj5, an endoplasmic reticulum (ER)-resident thioredoxin protein required as a disulfide reductase degradation misfolded in well-established Caenorhabditis elegans models Alzheimer, Parkinson Huntington...

Lifespan regulation by mitochondrial proteins has been well described, however, the mechanism of this is not fully understood. Amongst profoundly affecting ageing are prohibitins (PHB-1 and PHB-2). Paradoxically, in C. elegans prohibitin depletion shortens lifespan wild type animals while dramatically extending that metabolically compromised animals, such as daf-2-insulin-receptor mutants. Here we show amongst three kinases known to act downstream daf-2, only loss function sgk-1...

The study of mechanisms that govern feeding behaviour and its related disorders is a matter global health interest. roundworm Caenorhabditis elegans becoming model organism choice to these conserved pathways. C. depends on the contraction pharynx (pumping). Thanks worm transparency, pumping can be directly observed under stereoscope. Therefore, has been historically investigated by counting pharyngeal or other indirect approaches. However, those methods are short-term, time-consuming...

Abstract Cells can enter quiescence in adverse conditions and resume proliferation when the environment becomes favorable. Prolonged comes with a cost, reducing subsequent speed potential to return proliferation. Here, we show that similar process happens during Caenorhabditis elegans development, providing an vivo model study proliferative capacity after quiescence. Hatching under starvation provokes arrest of blast cell divisions normally take place first larval stage (L1). We have used...

Mitochondrial prohibitins (PHB) are highly conserved proteins with a peculiar effect on lifespan. While PHB depletion shortens lifespan of wild-type animals, it enhances longevity plethora metabolically compromised mutants, including target rapamycin complex 2 (TORC2) mutants sgk-1 and rict-1. Here, we show that have impaired mitochondrial homeostasis, lipogenesis yolk formation, plausibly due to alterations in membrane lipid sterol homeostasis. Remarkably, all these features suppressed by...

Advances in automated image-based microscopy platforms coupled with high-throughput liquid workflows have facilitated the design of large-scale screens utilising multicellular model organisms such as Caenorhabditis elegans to identify genetic interactions, therapeutic drugs or disease modifiers. However, analysis essential genes has lagged behind because lethal sterile mutations pose a bottleneck for approaches, and systematic way analyse interactions been lacking. In C. elegans,...

Mitochondrial function relies on the coordinated transcription of mitochondrial and nuclear genomes to assemble respiratory chain complexes. Across species, SIN3 coregulator influences functions, but how its loss impacts homeostasis metabolism in context a whole organism is unknown. Exploring this link important because

In a population, chemical communication determines the response of animals to changing environmental conditions, what leads an enhanced resistance against stressors. starvation, nematode Caenorhabditis elegans arrest post-embryonic development as L1 larva right after hatching. As arrested larvae, C. become more resistant diverse stresses, allowing them survive for several weeks expecting encounter favorable conditions. larvae secrete unidentified soluble compounds that improve survival...

Metabolic disorders are frequently associated with physiological changes that occur during ageing. The mitochondrial prohibitin complex (PHB) is an evolutionary conserved context-dependent modulator of longevity, which has been linked to alterations in lipid metabolism but biochemical function remains elusive. In this work we aimed at elucidating the molecular mechanism by depletion PHB shortens lifespan wild type animals while it extends insulin signaling receptor ( daf-2 ) mutants. A...

Abstract Heterochromatin is characterized by an enrichment of repetitive elements and low gene density often maintained in a repressed state across cell division differentiation. The silencing mainly regulated repressive histone marks such as H3K9 H3K27 methylated forms the heterochromatin protein 1 (HP1) family. Here, we analyzed tissue-specific manner binding profile two HP1 homologs Caenorhabditis elegans, HPL-1 HPL-2, at L4 developmental stage. We identified genome-wide intestinal...

ABSTRACT Background The advancement in automated image based microscopy platforms coupled with high throughput liquid workflows has facilitated the design of large scale screens utilizing multicellular model organisms such as Caenorhabditis elegans to identify genetic interactions, therapeutic drugs or disease modifiers. However, analysis essential genes lagged behind because lethal sterile mutations pose a bottleneck for approaches. Results In C. elegans, non-conditional can be maintained...

ABSTRACT In a population, chemical communication determines the response of animals to changing environmental conditions, what leads an enhanced resistance against stressors. starvation, nematode Caenorhabditis elegans arrest post-embryonic development as L1 larva right after hatching. As arrested larvae, C. become more resistant diverse stresses, allowing them survive for several weeks expecting encounter favorable conditions. However, prolonged periods in lead accumulation detrimental...

Abstract Heterochromatin is characterized by an enrichment of repetitive elements and low gene density often maintained in a repressed state across cell division differentiation. The silencing mainly regulated repressive histone marks, such as H3K9 H3K27 methylated forms the heterochromatin protein 1 (HP1) family. Here, we analyzed tissue-specific manner binding profile two HP1 homologs Caenorhabditis elegans , HPL-1 HPL-2, at L4 developmental stage. We identified genome-wide intestinal...

Mitochondrial function relies on the coordinated transcription of mitochondrial and nuclear genomes to assemble respiratory chain complexes. Across species, SIN3 coregulator influences functions, but how its loss impacts homeostasis metabolism in context a whole organism is unknown. Exploring this link important because haploinsufficiency causes intellectual disability/autism syndromes plays an role tumor biology. Here we show that C. elegans SIN-3 results transcriptional deregulation...

Abstract Ageing is characterized by physiological decline and increased risk of agerelated diseases. The mitochondrial prohibitin (PHB) complex, a ringlike structure in the inner membrane, critical to function proteostasis. Depletion PHB has opposite effects on ageing, shortening lifespan wildtype worms while extending different metabolically compromised animals, including that insulin/IGF-1like signalling (IIS) receptor mutants daf-2(e1370) . Lack strongly induces unfolded protein response...

Summary Cells can enter quiescence in adverse conditions and resume proliferation when the environment becomes favourable. Prolonged comes with a cost, reducing potential survival. Interestingly, cellular also occurs normal development, many cells spending most of their lifetime at this state. Elucidating mechanisms involved surviving long-term maintenance will contribute to better understanding process tissue regeneration. Developmental arrest C. elegans L1 stage is an emerging model for...