A5077590113- Pancreatic function and diabetes
- Mitochondrial Function and Pathology
- Diabetes and associated disorders
- Diet, Metabolism, and Disease
- Metabolism, Diabetes, and Cancer
- Adipose Tissue and Metabolism
- Radiomics and Machine Learning in Medical Imaging
- RNA modifications and cancer
- AI in cancer detection
- Bariatric Surgery and Outcomes
- Epigenetics and DNA Methylation
- Sarcoma Diagnosis and Treatment
- Genetic Syndromes and Imprinting
- Nuclear Receptors and Signaling
- GDF15 and Related Biomarkers
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Advanced X-ray and CT Imaging
- Diabetes Treatment and Management
- Macrophage Migration Inhibitory Factor
- Receptor Mechanisms and Signaling
- Environmental Justice and Health Disparities
- Sulfur Compounds in Biology
- Diagnosis and Treatment of Venous Diseases
- Nietzsche, Schopenhauer, and Hegel
- Atmospheric chemistry and aerosols
Aristotle University of Thessaloniki
1998-2025
Papageorgiou General Hospital
2020-2025
Imperial College London
2019-2024
Hammersmith Hospital
2020-2024
Metaxa Hospital
2020-2023
Genomics (United Kingdom)
2020
Infection et inflammation
2014
We identify biomarkers for disease progression in three type 2 diabetes cohorts encompassing 2,973 individuals across molecular classes, metabolites, lipids and proteins. Homocitrulline, isoleucine 2-aminoadipic acid, eight triacylglycerol species, lowered sphingomyelin 42:2;2 levels are predictive of faster towards insulin requirement. Of ~1,300 proteins examined two cohorts, GDF15/MIC-1, IL-18Ra, CRELD1, NogoR, FAS, ENPP7 associated with progression, whilst SMAC/DIABLO, SPOCK1 HEMK2...
Abstract Aims/hypothesis Mitochondrial oxidative metabolism is central to glucose-stimulated insulin secretion (GSIS). Whether Ca 2+ uptake into pancreatic beta cell mitochondria potentiates or antagonises this process still a matter of debate. Although the mitochondrial importer (MCU) complex thought represent main route for transport across inner membrane, its role in cells has not previously been examined vivo. Methods Here, we inactivated pore-forming subunit MCU, encoded by Mcu ,...
Mitochondrial glucose metabolism is essential for stimulated insulin release from pancreatic β-cells. Whether mitofusin gene expression, and hence, mitochondrial network integrity, important or incretin signaling has not previously been explored. Here, we generated mice with β-cell-selective, adult-restricted deletion knock-out (dKO) of the genes Mfn1 Mfn2 (βMfn1/2 dKO). βMfn1/2-dKO displayed elevated fed fasted glycemia a more than fivefold decrease in plasma insulin. length,...
Introduction Alzheimer’s disease (AD) even nowadays remains a complex neurodegenerative and its diagnosis relies mainly on cognitive tests which have many limitations. On the other hand, qualitative imaging will not provide an early because radiologist perceive brain atrophy late stage. Therefore, main objective of this study is to investigate necessity quantitative in assessment AD by using machine learning (ML) methods. Nowadays, ML methods are used address high dimensional data, integrate...
Abstract Aims/hypothesis Beta cells within the pancreatic islet represent a heterogenous population wherein individual sub-groups of make distinct contributions to overall control insulin secretion. These include subpopulation highly connected ‘hub’ cells, important for propagation intercellular Ca 2+ waves. Functional subpopulations have also been demonstrated in human beta with an altered subtype distribution apparent type 2 diabetes. At present, molecular mechanisms through which cell...
Coordination of cellular activity through Ca
Abstract Bariatric surgery improves both insulin sensitivity and secretion can induce diabetes remission. However, the mechanisms time courses of these changes, particularly impact on β cell function, are difficult to monitor directly. In this study, we investigated effect Vertical Sleeve Gastrectomy (VSG) function in vivo by imaging Ca 2+ dynamics islets engrafted into anterior eye chamber. Mirroring its clinical utility, VSG mice results significantly improved glucose tolerance, enhanced...
Aims/Hypothesis: The importance for normal insulin secretion of ceramide synthesis is unclear. De novo requires serine palmitoyl transferase, SPT2, encoded by Sptl2. Methods: We generated β-cell-selective Sptl2 null mice crossing animals with floxed alleles to expressing Cre recombinase from the Ins1 locus. Metabolic phenotyping, transcriptomic, functional analyses and histology were performed using standard approaches. Results: Islets Sptlc2∆Ins1 displayed marked alterations in...
Risk alleles for type 2 diabetes at the STARD10 locus are associated with lowered expression in β-cell, impaired glucose-induced insulin secretion, and decreased circulating proinsulin:insulin ratios. Although likely to serve as a mediator of intracellular lipid transfer, identity transported lipids thus pathways through which regulates β-cell function not understood. The aim this study was identify affected by role protein controlling proinsulin processing granule biogenesis maturation.We...
Background Cardiomegaly is a relatively common incidental finding on chest X-rays; if left untreated, it can result in significant complications. Using Artificial Intelligence for diagnosing cardiomegaly could be beneficial, as this pathology may underreported, or overlooked, especially busy under-staffed settings. Purpose To explore the feasibility of applying four different transfer learning methods to identify presence X-rays and compare their diagnostic performance using radiologists’...
Cystathionine beta synthase (CBS) catalyzes the first step of transsulfuration pathway from homocysteine to cystathionine, and its deficiency leads hyperhomocysteinemia (HHcy) in humans rodents. To date, scarce information is available about HHcy effect on insulin secretion, link between CBS activity setting type 2 diabetes still unknown. We aimed decipher consequences an inborn defect glucose homeostasis mice. used a mouse model heterozygous for (CBS+/−) that presented mild HHcy. Other...
Abstract Background The use of autologous tissues is considered the mainstay for delayed breast reconstruction. Aside free abdominal flaps, which are most commonly used, fat-augmented latissimus-dorsi (FALD) flap has been recently shown a reliable alternative option pure In this retrospective study, we aim to compare outcomes reconstructions using extended FALD and deep inferior epigastric perforator (DIEP) flap, with an emphasis on patients' characteristics, demographic data, complications,...
Abstract Pancreatic β-cells are responsible for production and secretion of insulin in response to increasing blood glucose levels. Defects β-cell function lead hyperglycemia diabetes mellitus. Here, we show that CNOT3, a CCR4–NOT deadenylase complex subunit, is dysregulated islets diabetic db/db mice, it essential murine β cell maturation identity. Mice with cell-specific Cnot3 deletion ( βKO) exhibit impaired tolerance, decreased mass, they gradually develop diabetes. βKO display...
Abstract Aims/hypothesis Although targeted in extrapancreatic tissues by several drugs used to treat type 2 diabetes, the role of AMP-activated protein kinase (AMPK) control insulin secretion is still debatable. Previous studies have pharmacological activators limited selectivity and specificity, none has examined primary pancreatic beta cells actions latest generation highly potent specific that act via allosteric drug metabolite (ADaM) site. Methods AMPK was activated acutely islets...
Abstract Aims/hypothesis Variants close to the VPS13C / C2CD4A/C2CD4B locus are associated with altered risk of type 2 diabetes in genome-wide association studies. While previous functional work has suggested roles for and C2CD4A disease development, none explored role C2CD4B . Methods CRISPR/Cas9-induced global C2cd4b -knockout mice zebrafish larvae c2cd4a deletion were used study this gene glucose homeostasis. C2 calcium dependent domain containing protein (C2CD)4A constructs tagged FLAG...