N6-methyladenosine (m6A) plays a crucial regulatory role in muscle growth and development. In our previous studies, we identified m6A methyltransferase, Methyltransferase like 16 (METTL16), which is associated with chicken development fiber type conversion. To further understand the of METTL16 function, analyzed its expression tissues different myofiber compositions primary myoblasts (CPMs) at various stages. We also manipulated CPMs to examine effects on cell proliferation, differentiation,...

Abstract Skeletal muscle fibers are primarily categorized into oxidative and glycolytic fibers, the ratios of different myofiber types important factors in determining livestock meat quality. However, molecular mechanism for fiber chickens was hardly understood. In this study, we used RNA sequencing to systematically compare mRNA microRNA transcriptomes sartorius (SART) pectoralis major (PMM) Chinese Qingyuan partridge chickens. Among 44,705 identified mRNAs two muscles, 3,457 exhibited...

MicroRNAs (miRNAs) might play critical roles in skeletal myofiber specification. In a previous study, we found that chicken miR-499-5p is specifically expressed slow-twitch muscle and its potential target gene SOX6. this performed RNA sequencing to investigate the effects of SOX6 on modulation regulation fiber type regulatory mechanism. The expression levels demonstrated opposing trends different muscles were associated with composition. Differential analysis revealed overexpression led...

Oxidative and glycolytic myofibers have different structures metabolic characteristics their ratios are important in determining poultry meat quality. However, the molecular mechanisms underlying differences unclear. In this study, global gene expression profiling was conducted oxidative skeletal muscle (obtained from soleus, or SOL) extensor digitorum longus, EDL) of Chinese Qingyuan partridge chickens, using Agilent Chicken Gene Expression Chip. A total 1224 genes with at least 2-fold were...

SART and PMM are mainly composed of oxidative myofibers glycolytic myofibers, respectively, myofiber types profoundly influence postnatal muscle growth meat quality. lncRNAs circRNAs that participate in type regulation. To elucidate the regulatory mechanism type, lncRNA circRNA sequencing was used to systematically compare transcriptomes Chinese female Qingyuan partridge chickens at their marketing age. The luminance value (L*), redness (a*), average diameter, cross-sectional area, density...

Abstract Back and thigh skin of chickens showed significant differences in the thickness feather follicle density size, which are important traits for slaughtered chickens' appearance. In present study, global gene expression profiling was conducted back using Microarray technology. The results that 676 genes were differentially expressed between skin. (DEGs), including PPP1R3C, IGF1, PTCHD1, HOXB6, FGF9, CAMK4, SHH, BMP8B, FOXN1 PTGER2, validated by real‐time quantitative polymerase chain...

AbstractTwenty four Chinese native duck breeds were studied to estimate genetic diversity and structure using microsatellite markers. The relationships between analyzed in combination with their geographic distribution. A total of 28 markers amplified. All the exhibited diversity, through estimation polymorphic information content (PIC) mean heterozygosity (H) index. populations was 0.569, while PIC values for relatively high. Neighbour-joining used calculate distances. Cluster analysis...

Abstract Genotyping plays an important role in breeding and population studies. Currently available genotyping technologies, including solid‐phase chips sequencing, often have several limitations their application to local chickens China, high costs, insufficient diversity, poor universality. In the present study, we developed a chicken 5 K single nucleotide polymorphism (SNP) array suitable for genetic analysis using by targeted sequencing technology. The chip design was based on genomic...

The comb of the male is an important secondary sexual characteristic. Although quantitative trait loci (QTLs) related to size have been identified, molecular mechanisms underlying this remain mostly unknown. In study, RNA sequencing (RNA-seq) was employed compare whole transcriptomic differences between two groups Partridge Shank chickens that are divergent in sizes. A total 563 differentially expressed genes (DEGs) were including 277 up-regulated and 286 down-regulated DEGs. According...

The mechanisms that regulate the specificity and maintenance of chicken muscle fiber types remain largely unknown. In mammals, CSRP3 has been shown to play a vital role in typical structure function. This study investigated plays skeletal muscle. First, antibody against protein was prepared, expression levels mRNA gene four muscles with different myofiber compositions were compared. Then effects silencing on profile myoblast transcriptomes analyzed. results showed both associated composition...

The pectoral muscle is an important component of skeletal muscle. blackness muscles can directly affect the economic value black-boned chickens. Although genes associated with melanogenesis in mammals and birds have been thoroughly investigated, only little known about key involved hyperpigmentation during embryonic development. Here, we analyzed melanin deposition patterns Yugan chickens compared differentially expressed (DEGs) between Wenchang (non-black-boned chickens) on days 9, 13, 17,...

This study was to detect the expression of heart fatty acid-binding protein (H-FABP) and adipocyte (A-FABP) gene mRNA in different tissues Rugao Luyuan chickens at 56 d 120 by real-time fluorescence quantitative reverse transcription polymerase-chain reaction (FQ-RT-PCR).The primers were designed according sequences H-FABP, A-FABP GAPDH genes Gallus gallus, which used as target internal reference gene, respectively.The levels H-FABP detected SYBR Green I FQ-RT-PCR.The relative level...

Differential lipid metabolic requirements of sexually-mature males and females may influence the regulation metabolism-associated genes hence content adipose tissue. We measured expression eight (fatty acid synthase, FASN; acylglycerol- 3- phosphate O-acyltransferase 9, AGPAT9; peroxisomal proliferator-activated receptor γ, PPARγ; lipoprotein lipase, LPL; carnitine palmitoyl transferase 1 A, CPT1A; B, CPT1B; acyl-COA dehydrogenase long chain, ACADL; monoglyceride MGL) in tissues...

The hydrolysis of the insecticide pyraclofos in buffered solutions at pH 5.0, 7.0 and 9.0, its sorption on four soils different physicochemical properties were investigated. results showed that degradation followed pseudo-first-order kinetics. At 40°C, rate constants for 9.0 0.0214, 0.1293, 2.1656 d−1, respectively. Pyraclofos was relatively stable under both acidic neutral conditions, while it readily hydrolyzed basic conditions. well described by Freundlich equation. constant, K f,...

TNNI1 encodes the slow skeletal muscle isoform of troponin I. In present study, basic characteristic and expressing profile gene was first explored in Gaoyou ducks. Full-length cDNA duck obtained using reverse transcription polymerase chain reaction (RT-PCR) rapid amplification ends (RACE). The consisted a 57-base pair (bp) 5'UTR, 345-bp 3'UTR, 564-bp open reading frame. predicted protein to be hydrophilic, nonsecretory contained 17 phosphorylation sites. Multiple alignments phylogenetic...

To investigate the population structure and genetic diversity of indigenous chicken breeds in Guizhou, a total 150 individual samples were collected from 12 breeds, including seven local Guizhou Province, three Chinese native found other provinces, two commercial breeds. The genotype datasets obtained using 50K single nucleotide polymorphism array method, then series analyses performed. parameters linkage disequilibrium decay indicated higher degree chickens than Two Wumeng black-bone...

Wooden breast (WB) is a muscle disorder affecting modern commercial broiler chickens that leads to palpable firm pectoralis major and causes severe reduction in meat quality, resulting substantial economic losses for the poultry industry. Most studies have focused on regulatory mechanisms underlying this defect with respect gene protein expression levels as well of metabolites. MicroRNAs (miRNAs) play critical roles human muscular disorders, such Duchenne dystrophy, by regulating...

The aim of this study was to identify genes involved in comb development provide insights into the molecular mechanism chickens' formation. Fixation index (FST) and average number base differences (π) males with large small combs were calculated based on whole-genome resequencing data. Chromosome regions larger FST values smaller π considered candidate selection regions. Through further annotation gene functions pathways, we sought screen possible selected associated development. By...

The COⅠ genes in two newly discovered chicken breeds(Jinhu silky and Wahui) indigenous breed(Langshan) were amplified by sequencing,in order to post the genetic diversity of these resources their heredity relation with other breeds.The results showed that selective sequence COⅠhad 10 SNPs 7 haplotypes,3 specific mutation sites,5 haplotypes haplotypes.The average nucleotide diversities Jinhu silky,Wahui Langshan 0.068%,0.051% 0.0369%,respectively;the haplotype them 0.4167,0.3030...

Twenty four Chinese native duck breeds were studied to estimate genetic diversity and structure using microsatellite markers. The relationships between analyzed in combination with their geographic distribution. A total of 28 markers amplified. All the exhibited diversity, through estimation polymorphic information content (PIC) mean heterozygosity (H) index. populations was 0.569, while PIC values for relatively high. Neighbour-joining used calculate distances. Cluster analysis divided...