A5040085318- Escherichia coli research studies
- Amphibian and Reptile Biology
- Viral gastroenteritis research and epidemiology
- Enterobacteriaceae and Cronobacter Research
- Milk Quality and Mastitis in Dairy Cows
- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Turtle Biology and Conservation
- Bacteriophages and microbial interactions
- Species Distribution and Climate Change
- Animal Disease Management and Epidemiology
- Insect and Pesticide Research
- Plant and animal studies
- Clostridium difficile and Clostridium perfringens research
- Bacterial biofilms and quorum sensing
- Plant Pathogens and Fungal Diseases
- Food Safety and Hygiene
- Dermatological diseases and infestations
- Parasitic Infections and Diagnostics
- Identification and Quantification in Food
- Cystic Fibrosis Research Advances
- Forensic Entomology and Diptera Studies
- Insect symbiosis and bacterial influences
Universidade Estadual de Campinas (UNICAMP)
2015-2025
Universidade Estadual Paulista (Unesp)
2020
Hospital de Clínicas da Unicamp
2002-2012
Abstract The amphibian fungal disease chytridiomycosis, which affects species across all continents, recently emerged as one of the greatest threats to biodiversity. Yet, many aspects basic biology and epidemiology pathogen, Batrachochytrium dendrobatidis ( Bd ), are still unknown, such when from where did emerge what is its true ecological niche? Here, we review ecology evolution in Americas highlight controversies that make this so enigmatic. We explore factors associated with variance...
Chytridiomycosis, caused by the fungus Batrachochytrium dendrobatidis (Bd), is emerging infectious disease implicated in recent population declines and extinctions of amphibian species worldwide. Bd strains from regions disease-associated decline to date have all belonged a single, hypervirulent clonal genotype (Bd-GPL). However, earlier studies Atlantic Forest southeastern Brazil detected novel, putatively enzootic lineage (Bd-Brazil), indicated hybridization between Bd-GPL Bd-Brazil. Here,...
Adhesins (P-fimbriae, S-fimbriae, type 1 fimbriae and afimbrial adhesin), toxins (α-hemolysin cytotoxic necrotizing factor 1), iron acquisition systems (aerobactin) host defense avoidance mechanisms (capsule or lipopolysaccharide) have been shown to be prevalent in Escherichia coli strains associated with urinary tract infections. In this work, 162 Uropathogenic (UPEC) from patients cystitis were genotypically characterized by polymerase chain reaction (PCR) assay. We developed three...
Genomic studies of the amphibian-killing fungus (Batrachochytrium dendrobatidis, [Bd]) identified three highly divergent genetic lineages, only one which has a global distribution. Bd strains within these linages show variable genomic content due to differential loss heterozygosity and recombination. The current quantitative polymerase chain reaction (qPCR) protocol detect from amphibian skin swabs targets intergenic transcribed spacer 1 (ITS1) region using TaqMan fluorescent probe specific...
The 'dilution effect' (DE) hypothesis predicts that diverse host communities will show reduced disease. underlying causes of pathogen dilution are complex, because they involve non-additive (driven by interactions and differential habitat use) additive (controlled species composition) mechanisms. Here, we used measures complementarity selection traditionally employed in the field biodiversity-ecosystem function (BEF) to quantify net effect diversity on disease dynamics amphibian-killing...
Habitat fragmentation and wildlife disease are two widespread drivers of biodiversity loss, yet few empirical studies have explored their interactions. In this study, we utilized a naturally fragmented island system to examine the impacts on genetic diversity amphibian infection dynamics. We determined at immunity locus MHC IIB, hypothesized predictor susceptibility. Contrary expectations, populations lost while simultaneously experiencing positive selection IIB. then used Next-Generation...
Bullfrog farming and trade practices are well-established, globally distributed, economically valuable, but pose risks for biodiversity conservation. Besides their negative impacts on native amphibian populations as an invasive species, bullfrogs play a key role in spreading the frog-killing fungus Batrachochytrium dendrobatidis (Bd) natural environment. Bullfrogs tolerant to Bd, meaning that they can carry high infection loads without developing chytridiomycosis. To test potential of...
Two hundred and five Escherichia coli strains isolated from calves with diarrhea mid-western Brazil were screened for the presence of virulence factors associated bovine colibacillosis. One two (49.8%) E. produced toxins: Shiga toxins 1 (9.7%) 2 (6.3%), a-hemolysin (9.7%), enterohemolysin(6.8%), Cytotoxic Necrotizing Factors type (0.5%), (4.4%), enterotoxins LT-II (8.3%) STa (3.9%). No strain enterotoxin LT-I. Fimbrial adhesins F5 F17 by 7.3% 4.8% strains, respectivly, none expressed F41....
Enigmatic amphibian declines were first reported in southern and southeastern Brazil the late 1980s included several species of stream-dwelling anurans (families Hylodidae Cycloramphidae). At that time, we unaware amphibian-killing fungus Batrachochytrium dendrobatidis (Bd); therefore, pollution, habitat loss, fragmentation unusual climatic events hypothesized as primary causes these declines. We now know multiple lineages Bd have infected amphibians Brazilian Atlantic forest for over a...
A total of 120 strains Pseudomonas aeruginosa, isolated from cystic fibrosis (CF) patients (n = 80) and having extra-pulmonary infections 40) were studied regarding the presence some virulence factors (hemolysin, gelatinase elastase production) algD algU genes as detected by polymerase chain reaction-PCR. There was not a significant difference for production hemolysin between non-mucoid CF other isolates mucoid strains. The found to be among these gene PCR in all but only 25% Conclusion...
Escherichia coli sfa + strains isolated from poultry were serotyped and characterized by polymerase chain reaction (PCR) amplified fragment length polymorphism (AFLP). Isolates collected 12 Brazilian farms mostly belonged to serogroup O6, followed serogroups O2, O8, O21, O46, O78, O88, O106, O111, O143. Virulence genes associated were: iuc 90%, fim 86% neuS 60%, hly 34%, tsh 28%, crl/csg 26%, iss pap 18%, 14% cnf . Strains the same farm presented more than one genotypic pattern belonging...
Abstract In Brazil’s Atlantic Forest (AF) biodiversity conservation is of key importance since the fungal pathogen Batrachochytrium dendrobatidis (Bd) has led to rapid loss amphibian populations here and worldwide. The impact Bd on amphibians determined by host's immune system, which skin microbiome a critical component. richness diversity such cutaneous bacterial communities are known be shaped abiotic factors thus may indirectly modulate host susceptibility . This study aimed contribute...
Mammary pathogenic Escherichia coli (MPEC) is one of the most common pathogens associated with clinical mastitis. We analyzed isolates obtained from milk samples cows mastitis, collected 10 farms in Brazil, to verify molecular and phenotypic characteristics. A total 192 (4.5%) mammary E. were 4,275 analyzed, but we tested 161. assigned these phylogroups B1 (52.8%) (36.6%), although B2, C, D, E, unknown also occurred. All assessed for presence several genes encoding virulence factors, such as...
Altamira city, in the north of Brazil, is known to be a support region since construction Belo Monte Hydroelectric Complex. The Xingu River permeates being extremely important as tributary Amazon River. Only portion population provided with basic sanitation, and alternatively use water wells septic tanks, which can sources contamination. study sought understand seasonal variation fecal contamination groundwater resources. households were divided into six zones, from 30 sampled dry wet...
The fungus Batrachochytrium dendrobatidis (Bd) is among the main causes of declines in amphibian populations. This considered a generalist pathogen because it infects several species and spreads rapidly wild. To date, Bd has been detected more than 100 anuran Brazil, mostly southern portion Atlantic forest. Here, we report survey data from some poorly explored regions; these considerably extend current information on distribution northern forest region. In addition, tested hypothesis that...