A5006151405- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Multiple Myeloma Research and Treatments
- Pneumonia and Respiratory Infections
- Genomics and Chromatin Dynamics
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- CAR-T cell therapy research
- Prostate Cancer Treatment and Research
- DNA and Nucleic Acid Chemistry
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA Research and Splicing
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Prostate Cancer Diagnosis and Treatment
- Reproductive tract infections research
- RNA modifications and cancer
- Garlic and Onion Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Photochromic and Fluorescence Chemistry
- Advancements in Semiconductor Devices and Circuit Design
- Advanced biosensing and bioanalysis techniques
University of Virginia
2017-2025
University of the Virgin Islands
2023
C4 Therapeutics (United States)
2021
Office of Public Health Genomics
2020
Dana-Farber Cancer Institute
2016-2019
Harvard University
2017
National Institutes of Health
2013
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2013
Jarvis Christian College
1999
Technological University Dublin
1996-1997
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthetic spectroscopic models related to coenzymes and base pairs. V. Emission properties of NADH. Studies fluorescence lifetimes quantum efficiencies NADH, AcPyADH, [reduced acetylpyridineadenine dinucleotide] simplified synthetic modelsT. Gordon Scott, Richard D. Spencer, Nelson J. Leonard, Gregorio WeberCite this: Am. Chem. Soc. 1970, 92, 3, 687–695Publication Date (Print):February 1, 1970Publication History Published online1 May 2002Published...
Chemical inhibition of epigenetic regulatory proteins BrdT and Brd4 is emerging as a promising therapeutic strategy in contraception, cancer, heart disease. We report an easily synthesized dihydropyridopyrimidine pan-BET inhibitor scaffold, which was uncovered via virtual screen followed by testing fluorescence anisotropy assay. Dihydropyridopyimidine 3 subjected to further characterization highly selective for the BET family bromodomains. Structure–activity relationship data ligand...
Common genetic variants in the repressive GATA-family transcription factor (TF) TRPS1 locus are associated with breast cancer risk, and luminal cell lines particularly sensitive to knockout. We introduced an inducible degron tag into native within a line identify direct targets of determine how mechanistically regulates gene expression. acutely deplete over 80 percent from chromatin 30 minutes inducing degradation. find that hundreds genes, including those related estrogen signaling....
A ferrocene containing o-aminoanilide, N1-(2-aminophenyl)-N8-ferrocenyloctanediamide (2b, Pojamide) displayed nanomolar potency vs HDAC3. In comparison to RGFP966, a potent and selective HDAC3 inhibitor, Pojamide superior activity in HCT116 colorectal cancer cell invasion assays; however, TCH106 romidepsin, HDAC1 inhibitors, outperformed cellular proliferation colony formation assays. Together, these data suggest that HDAC1,3 inhibition is desirable achieve maximum anticancer benefits....
Abstract The clinical success of combined androgen deprivation therapy (ADT) and radiotherapy (RT) in prostate cancer created interest understanding the mechanistic links between receptor (AR) signaling DNA damage response (DDR). Convergent data have led to a model where AR both regulates, is regulated by, DDR. Integral this that regulates transcription DDR genes at steady state ionizing radiation (IR). In study, we sought determine which immediate transcriptional changes are induced by IR...
Direct transfer of proteins between DNA helices is a recognized important feature the recognition site search process. characterized by dissociation rate that depends on total concentration. This taken as evidence for formation an intermediate DNA-protein-DNA ternary complex. We find EcoRI-DNA-specific complexes at 80 mM NaCl concentration competitor oligonucleotide suggesting direct contributes to EcoRI dissociation. dependence not seen 180 salt. A careful examination salt rate, however,...
Gastrointestinal stromal tumor (GIST) is a mesenchymal neoplasm characterized by activating mutations in the related receptor tyrosine kinases KIT and PDGFRA. GIST relies on expression of these unamplified kinase (RTK) genes through large enhancer domain, resulting high levels oncogene required for growth. Although inhibition an effective therapy many patients with GIST, disease progression from kinase-resistant common no other classes systemic exist. In this study, we identify regulatory regions
In addition to the therapeutic applicability of targeted protein degradation (TPD), modality also harbors unique properties that enable development innovative chemical biology tools interrogate complex biology. TPD offers an all-chemical strategy capable potent, durable, selective, reversible, and time-resolved control levels a given target in both vitro vivo contexts. These are particularly well-suited for enabling precise perturbation gene understand its biology, identify...
Moraxella catarrhalis adheres to human erythrocytes by means of a proteinaceous, trypsin sensitive, heat modifiable haemagglutinin. A 200 kDa protein was found be associated with haemagglutinating isolates M. catarrhalis. This present on all (n=17), but absent the non-haemagglutinating (n=23) examined. demonstrated heat-modifiable properties in sodium dodecyl sulfate and degraded trypsin. Immunoblot assays polyclonal antiserum indicated that exclusively antibodies this did not recognise...
Abstract Precision genomic run-on assays (PRO-seq) quantify nascent RNA at single nucleotide resolution with strand specificity. Here we deconstruct a recently published processing pipeline (PEPPRO) into its components and link the analyses to underlying molecular biology. PRO-seq experiments are evolving variations can be found throughout literature. The presented as individual code chunks comprehensive details so that users modify framework accommodate different protocols. We present...
A total of 40 strains Moraxella catarrhalis, isolated from the sputum elderly persons with respiratory tract infections and nasopharyngeal swabs healthy elderly, were examined for haemagglutination human red blood cells resistance to bactericidal activity in normal serum (NHS). 15 20 infected 3 showed haemaggluti-nating properties (p < 0.001). Similarly, 13 group 2 found be resistant NHS Strains M. catarrhalis which associated contrast colonizing predominantly haemagglutinating complement...
Haemagglutination and tissue culture adherence tests using a McCoy cell line were used to examine the characteristics of 105 strains Gardnerella vaginalis. Each strain represented one isolate per patient. For each patient, direct smear vaginal discharge was examined for clue cells. The relation between in vitro presence cells examined. There seemed be no appreciable smears haemagglutinating activity strains. In contrast, as judged by system showed significant (p less than 0.001). Though both...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpectroscopic models related to coenzymes and base pairs. I. Basis for hypochromism in the ultraviolet spectra of model systems nicotinamide adenine dinucleotideNelson J. Leonard, Thomas Gordon. Scott, Ping Cheong. HuangCite this: Am. Chem. Soc. 1967, 89, 26, 7137–7138Publication Date (Print):December 1, 1967Publication History Published online1 May 2002Published inissue 1 December...
Inducible degron systems are widely used to specifically and rapidly deplete proteins of interest in cell lines organisms. An advantage inducible degradation is that the biological system under study remains intact functional until perturbation, a feature necessitates endogenous levels protein maintained. However, tagging genes with auxin-inducible degrons (AID) can result chronic, auxin-independent proteasome-mediated degradation. The ARF-AID (auxin-response factor-auxin-inducible degron)...
Transcription factors bind to sequence motifs and act as activators or repressors. interface with a constellation of accessory cofactors regulate distinct mechanistic steps transcription. We rapidly degraded the essential ubiquitously expressed transcription factor ZNF143 determine its function in cycle. facilitates RNA Polymerase initiation activates gene expression. binds promoter nearly all activated target genes. also near site genic directly repress subset Although stimulates at...
Transcription factors bind to sequence motifs and act as activators or repressors. interface with a constellation of accessory cofactors regulate distinct mechanistic steps transcription. We rapidly degraded the essential pervasively expressed transcription factor ZNF143 determine its function in cycle. facilitates RNA polymerase initiation activates gene expression. binds promoter nearly all activated target genes. also near site genic directly repress subset Although stimulates at...
The haemagglutination and tissue culture adherence properties of 20 isolates Moraxella catarrhalis obtained from the sputum elderly patients with lower respiratory tract infections were compared those M. nasopharynx persons colonised by organism. Eighty percent infected group as opposed to 5% haemagglutinated human erythrocytes (P<0.001), indicating that haemagglutinin might be a marker pathogenicity for catarrhalis. There was significant difference in HEp-2 cells comparison (P=0.03)....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthetic spectroscopic models related to coenzymes and base pairs. X. Quaternized bisnicotinamidesJohn H. Craig, Ping C. Huang, T. Gordon Scott, Nelson J. LeonardCite this: Am. Chem. Soc. 1972, 94, 16, 5872–5879Publication Date (Print):August 1, 1972Publication History Published online1 May 2002Published inissue 1 August 1972https://pubs.acs.org/doi/10.1021/ja00771a055https://doi.org/10.1021/ja00771a055research-articleACS PublicationsRequest reuse...