A5102733453- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
Universität Hamburg
2021-2022
Heterozygous mutations in six transfer RNA (tRNA) synthetase genes cause Charcot-Marie-Tooth (CMT) peripheral neuropathy. CMT mutant tRNA synthetases inhibit protein synthesis by an unknown mechanism. We found that glycyl-tRNA bound tRNAGly but failed to release it, resulting sequestration. This sequestration potentially depleted the cellular pool, leading insufficient glycyl-tRNAGly supply ribosome. Accordingly, we ribosome stalling at glycine codons and activation of integrated stress...
Transfer RNAs (tRNAs) are highly abundant species and, along their biosynthetic and functional path, they establish interactions with a plethora of proteins. The high number nucleobase modifications in tRNAs renders conventional RNA quantification approaches unsuitable to study protein-tRNA associated roles the cell. We present an immunoprecipitation-based approach quantify tRNA bound its interacting protein partner(s). tRNA-protein complexes immunoprecipitated from cells or tissues...