A5079452452- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Alzheimer's disease research and treatments
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Prion Diseases and Protein Misfolding
- Photosynthetic Processes and Mechanisms
- Epigenetics and DNA Methylation
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA modifications and cancer
- Algal biology and biofuel production
- Genomics and Chromatin Dynamics
- Bacteriophages and microbial interactions
- Graphene research and applications
- Biocrusts and Microbial Ecology
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Nanopore and Nanochannel Transport Studies
- SARS-CoV-2 detection and testing
- Microfluidic and Bio-sensing Technologies
- Synthesis and Biological Activity
- Molecular Communication and Nanonetworks
Tokyo University of Agriculture and Technology
2015-2024
John Wiley & Sons (United States)
2014-2017
Bioengineering Center
2014-2017
National Center for Genetic Engineering and Biotechnology
2014-2017
Hudson Institute
2017
RIKEN Center for Brain Science
2014
α-Synuclein (α-syn) oligomers are considered major molecules responsible for the onset of Parkinson's disease and dementia with Lewy bodies. α-Syn thus serve as an important target development drugs diagnostic tests neurodegenerative diseases. In this paper we report on identification DNA aptamers that bind to soluble α-syn oligomers. A competitive screening method based aptamer blotting was used selection oligomer-specific aptamers. This approach resulted in eight specifically among...
ABSTRACT Proteus mirabilis is a prominent cause of catheter‐associated urinary tract infections (CAUTIs) among patients undergoing long‐term bladder catheterization. There are currently no effective means preventing P. infections, and strategies for prophylaxis rapid early diagnosis urgently required. Aptamers offer significant potential development countermeasures against CAUTI an ideal class molecules the diagnostics therapeutics. Here we demonstrate application Cell‐SELEX to identify DNA...
Mammary tumor–associated amyloidosis (MTAA) in dogs is characterized by amyloid deposition the stroma of mammary adenoma or carcinoma; however, precursor protein remains unknown. We attempted to identify an and elucidated its etiology characterizing 5 cases canine MTAA. Proteomic analyses extracts from formalin-fixed paraffin-embedded specimens revealed α-S1-casein (CASA1) as a prime candidate showed N-terminal truncation CASA1. Both immunohistochemistry immunoelectron microscopy that...
DNA–protein complexes are attractive components with broad applications in various research fields, such as DNA aptamer–enzyme biosensing elements. However, noncovalent often decrease detection sensitivity because they highly susceptible to environmental conditions. In this study, we developed a versatile covalent-linking patch (D-Pclip) for fabricating covalent and stoichiometric complexes. We comprehensively explored the database determine DNA-binding ability of candidates selected UdgX...
Host plant selection may depend on the metabolic system in herbivorous insects. Although oligophagous insects take up specific host components, how components and their biological activities are altered through systems remains unknown. Here, by examining gene expression of enzymes larval frass, we investigated ability Papilio memnon larvae fed with Citrus x paradisi (grapefruit) against components. The levels some were fluctuated between midgut fat bodies. Furthermore, chloroform extract...
A method for detecting CpG methylation is required in clinical settings because associated with various diseases. leads to structural changes single-stranded DNA and also...
G-quadruplex (G4) is a DNA/RNA conformation that consists of two or more G-tetrads resulting from four-guanine bases connected by Hoogsteen-type hydrogen bonds, which often found in the telomeres chromatin, as well promoter regions genes. The function G4 genomic DNA being elucidated and some G4-protein interactions have been reported; these are believed to play role vital cellular functions. In this study, we focused on CpG methylation, well-known epigenetic modification DNA, especially...
We propose a highly selective, sensitive, accurate, and high-throughput bioanalysis method for bevacizumab utilizing an anti-idiotype DNA aptamer. With this method, in plasma sample was reacted 96-well plate immobilized with the aptamer further protein A–HRP conjugate. The resulting HRP activity colorimetrically detected using microplate reader. calibration curve of ranged from 0.05 to 5.0 μg/mL, showed good correlation coefficient (r2 = 1.000). limit detection 2.09 ng/mL. also demonstrated...
In silico evolution with an in vitro system can facilitate the development of functional aptamers high specificity and affinity. Although a general technique known as systematic ligand by exponential enrichment (SELEX) is efficient method for aptamer selection, it sometimes fails to identify sufficient binding properties. We have previously developed maturation (ISM) improve functions based on genetic algorithms. ISM represents intelligent exploitation random search within defined sequence...
By the binding of a G4 ligand to G4-forming aptamers, their conformations became suitable for target and ability increased.
Single-strand oligodeoxynucleotides (ODNs) containing unmethylated cytosine-phosphate-guanine (CpG) are recognized by the toll-like receptor 9, a component of innate immunity. Therefore, they could act as immunotherapeutic agents. Chemically modified CpG ODNs phosphorothioate backbone instead phosphodiester (PD) were developed agents resistant to nuclease degradation. However, cause adverse side effects, and so there is necessity generate novel ODNs. In present study, we designed...
Abstract Aptamers can control the biological functions of enzymes, thereby facilitating development novel biosensors. While aptamers that inhibit catalytic reactions enzymes were found and used as signal transducers to sense target molecules in biosensors, no amplify enzymatic activity have been identified. In this study, we report G-quadruplex (G4)-forming DNA upregulate peroxidase myoglobin specifically for luminol. Using vitro selection, one G4-forming aptamer enhanced chemiluminescence...
This study presents a simple, accurate, and selective bioanalytical method of bevacizumab detection from plasma samples based on aptamer affinity purification⁻high-temperature reversed-phased liquid chromatography (HT-RPLC) with fluorescence detection. Bevacizumab in was purified using magnetic beads immobilized an anti-idiotype DNA for bevacizumab. The separated HT-RPLC detected its native fluorescence. Using beads, selectively as single peak the chromatogram. achieved good separation sharp...
For realizing the early diagnosis of diseases, detection gases in exhaled breath parts per billion (ppb) using compact devices is necessary. Herein, single‐stranded‐DNA‐modified graphene field‐effect transistors (FETs) are fabricated and transfer characteristics measured. The results reveal that shifts obtained by introducing ethanol gas at ppb level, indicating FETs detect level. Moreover, modifying DNA sequence 1 or 2, positive negative voltage observed, respectively. In contrast, hardly...
We report on the development of a versatile and accurate bioanalytical method for bevacizumab using pretreatment combining affinity purification with anti-idiotypic DNA aptamers centrifugal ultrafiltration concentration, followed by liquid chromatography (LC)-fluorescence analysis. An Sepharose beads as an support removed immunoglobulin G large amount coexisting substances in serum sample. Purified was separated single peak conventional LC detected fluorometrically, showing good linearity...
Several neurodegenerative diseases have a common pathophysiology where selective damage to neurons results from the accumulation of amyloid oligomer proteins formed via fibrilization. Considering that formation oligomers leads cytotoxicity, development chemical compounds are able effectively cross blood-brain barrier (BBB) and inhibit this conversion and/or fibrils is essential for disease therapy. We previously reported pyrroloquinoline quinone (PQQ) prevented aggregation fibrillation...
Aptamer-based electrochemical sensors have gained attention in the context of developing a diagnostic biomarker detection method because their rapid response, miniaturization ability, stability, and design flexibility. In such systems, enzymes are often used as labels to amplify signal. We focused on glucose dehydrogenase (GDH) labeling enzyme for owing its high enzymatic activity, availability, well-established principle platform. However, it is difficult laborious obtain one GDH-aptamer...
Amyloidosis is characterized by the abnormal accumulation of amyloid proteins. The causative proteins aggregate from monomers to oligomers and fibrils, among which some intermediate are considered as major toxins. Cytotoxic generated not only aggregation but also via fibril disaggregation. However, little known about structural characteristics generation conditions cytotoxic produced during Herein, we summarized commonalities formed under various disaggregation conditions, including addition...