A5020626893- Bladder and Urothelial Cancer Treatments
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Brain Metastases and Treatment
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- Management of metastatic bone disease
- Spine and Intervertebral Disc Pathology
- DNA Repair Mechanisms
- Medical Imaging Techniques and Applications
- Spinal Fractures and Fixation Techniques
- Lung Cancer Treatments and Mutations
- Peptidase Inhibition and Analysis
- Cancer Genomics and Diagnostics
- Health Systems, Economic Evaluations, Quality of Life
- Advanced Radiotherapy Techniques
- Radiomics and Machine Learning in Medical Imaging
- Radiopharmaceutical Chemistry and Applications
- Head and Neck Cancer Studies
- Frailty in Older Adults
- Effects of Radiation Exposure
- Chronic Myeloid Leukemia Treatments
- Cancer-related Molecular Pathways
- Gastrointestinal Tumor Research and Treatment
Institute of Oncology Ljubljana
2018-2024
University of Ljubljana
2007-2024
CRUK/MRC Oxford Institute for Radiation Oncology
2013-2017
University of Oxford
2012-2017
Ontario Institute for Cancer Research
2013
Princess Margaret Cancer Centre
2013
University of Toronto
2013
University of Bradford
2013
The MRE11/RAD50/NBS1 (MRN) complex mediates DNA repair pathways, including double-strand breaks induced by radiotherapy. Meiotic recombination 11 homolog (MRE11) is downregulated histone deacetylase inhibition (HDACi), resulting in reduced levels of bladder cancer cells and radiosensitization. In this study, we show that the mechanism downregulation posttranslational identify a C-terminally truncated MRE11, which formed after HDAC as full-length MRE11 downregulated. Truncated was stabilized...
As the population ages, more elderly patients require radiotherapy-based treatment for their pelvic malignancies, including muscle-invasive bladder cancer, as they are unfit major surgery. Therefore, there is an urgent need to find radiosensitizing agents minimally toxic normal tissues, bowel and bladder, such patients. We developed methods determine tissue toxicity severity in intestine
In muscle-invasive bladder cancer there is an urgent need to identify relatively non-toxic radiosensitising agents for use in elderly patients. Histone deacetylase inhibitors radiosensitise tumour cells but not normal vitro and variously downregulate DNA damage signalling, homologous recombination (HR) non-homologous end-joining (NHEJ) repair proteins. We investigated panobinostat (PAN) as a potential radiosensitiser cells.
Radiotherapy is a major treatment modality used to treat muscle-invasive bladder cancer, with patient outcomes similar surgery. However, radioresistance significant factor in failure. Cell-free extracts of tumors are defective nonhomologous end-joining (NHEJ), and this phenotype may be clinically by combining radiotherapy radiosensitizing drug that targets homologous recombination, thereby sparing normal tissues intact NHEJ. The response the recombination protein RAD51 radiation inhibited...
Treatment options for recurrent head and neck tumours in the previously irradiated area are limited, including re-irradiation due to radioresistance of tumour previous dose received by surrounding normal tissues. As an vitro model study mechanisms, isogenic cells with different radiosensitivity can be used. However, they not readily available. Therefore, our objective was establish characterize radioresistant human pharyngeal squamous carcinoma evaluate early radiation response parental,...
Patients with metastatic epidural spinal cord compression (MESCC) and favorable survival prognoses may benefit from radiation doses exceeding 10 × 3.0 Gy. In a multi-center phase 2 trial, patients receiving 15 2.633 Gy (41.6 Gy10) or 18 2.333 (43.2 were evaluated for local progression-free (LPFS), motor/sensory functions, ambulatory status, pain, distress, toxicity, overall (OS). They compared (propensity score-adjusted Cox regression) to historical control group (n = 266) (32.5 Gy10). the...
In a recent phase II clinical trial, low-dose (100 mg/m(2)) gemcitabine showed promise as radiosensitizer in bladder cancer, but underlying mechanisms lack elucidation. Here, we investigated the mechanism of radiosensitization by cancer cell lines.
Electrochemotherapy is an established local ablative method used for the treatment of different tumor types, including tumors head and neck area. Clinical studies have demonstrated a lower response rate that recur in pre‑irradiated The aim present study was to explore experimentally induced radioresistant cells electrochemotherapy with cisplatin or bleomycin. (FaDu‑RR) were by fractionated irradiation parental human squamous cell carcinoma line, FaDu. We compared 2 lines chemotherapy...
Patients with metastatic epidural spinal cord compression (MESCC) and favorable survival prognoses may benefit from radiation doses exceeding 10 x 3.0 Gy. In a multi-center phase 2 trial, patients receiving 15 2.633 Gy (41.6 Gy10) or 18 2.333 (43.2 were evaluated for local progression-free (LPFS), motor/sensory functions, ambulatory status, pain, distress, toxicity, overall (OS). They compared (propensity score adjusted Cox regression) to historical control group (N=266) (32.5 Gy10). the...
Human papillomavirus (HPV) is an important etiological factor in oropharyngeal squamous cell carcinoma (SCC). Compared to HPV-negative tumors, HPV-positive SCC has shown a better response nonsurgical treatments. In this study, we determined the dose-modifying factors for tumors with single-dose irradiation, or without low radiosensitizing doses of cisplatin.In vitro, increased radiosensitivity SCC, which might be consequence HPV-induced changes cycle regulation and DNA damage response,...
Abstract Background Management of locoregionally recurrent head and neck squamous cell carcinomas (HNSCC) is challenging due to potential radioresistance. Pulsed low-dose rate (PLDR) irradiation exploits phenomena increased radiosensitivity, hyperradiosensitivity (LDHRS), inverse dose-rate effect. The purpose this study was evaluate LDHRS the effect PLDR in isogenic HNSCC cells with different radiosensitivity. Materials methods Cell survival after regimens parental FaDu radioresistant...
Estimating post-treatment ambulatory status can improve treatment personalization of patients irradiated for malignant spinal cord compression (MSCC). A new clinical score was developed from data 283 treated with radiotherapy alone in prospective trials. Radiotherapy regimen, age, gender, tumor type, interval diagnosis to MSCC, number affected vertebrae, other bone metastases, visceral time developing motor deficits, status, performance score, sensory and sphincter dysfunction were...
Many patients with malignant spinal cord compression (MSCC) who are not candidates for neurosurgery receive radiotherapy alone. This study compared 15 × 2.633 Gy over three weeks to 20 2.0 four in favorable survival prognoses. The outcomes of 34 treated (equivalent dose 41.6
Increased radioresistance due to previous irradiation or radiosensitivity human papilloma virus (HPV) infection can be observed in head and neck squamous cell carcinoma (HNSCC). The DNA-damage response of cells after exposure DNA-damaging agents plays a crucial role determining the fate exposed cells. Tightly regulated interconnected signaling networks are activated detect, signal presence repair DNA damage. Novel therapies targeting emerging; however, an improved understanding complex...
For optimal personalization of treatment for metastatic spinal cord compression (MSCC), the patient’s survival prognosis should be considered. Estimation can facilitated by prognostic factors. This study investigated value pre-treatment preclinical markers, namely hemoglobin, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR), lactate dehydrogenase (LDH), and c-reactive protein (CRP), in 190 patients from two prospective trials who had poor or intermediate prognoses were...
Blood phenylalanine-to-tyrosine (Phe/Tyr) ratio is an important indicator of metabolic control in phenylketonuria patients. We present the data that highlights role Phe/Tyr-ratio evaluation tetrahydrobiopterin (BH4)-responsiveness patients with hyperphenylalaninemia. Our complements results from original research article by Tansek et al., 2012 [1]. performed a BH4-loading test 32 after four days increased protein intake (2000 mg/kg body weight). sampling was 96, 72, 48, 24, 16 h, and moments...
<p>Supplementary Figure S4. Clonogenic plating efficiency of HUVECs in the presence gemcitabine.</p>