A5059478665- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Yersinia bacterium, plague, ectoparasites research
- Long-Term Effects of COVID-19
- Transgenic Plants and Applications
- Animal Virus Infections Studies
- vaccines and immunoinformatics approaches
- Extracellular vesicles in disease
- PARP inhibition in cancer therapy
- Viral gastroenteritis research and epidemiology
- Insect and Pesticide Research
- Bee Products Chemical Analysis
- Bacillus and Francisella bacterial research
- SARS-CoV-2 detection and testing
- Cannabis and Cannabinoid Research
- Aviation Industry Analysis and Trends
- Vibrio bacteria research studies
University of Louisville
2020-2023
Targeting the main protease of SARS-CoV-2 Inside host cells, RNA genome severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is translated into two polyproteins that are cleaved to give individual viral proteins. The protease, known as Mpro or 3CLpro, plays a key role in these cleavages, making it an important drug target. Drayman et al . identified eight drugs target 3CLpro from library 1900 clinically safe drugs. Because challenge working with SARS-CoV-2, they started by screening...
The spread of SARS-CoV-2 and ongoing COVID-19 pandemic underscores the need for new treatments. Here we report that cannabidiol (CBD) inhibits infection in cells mice. CBD its metabolite 7-OH-CBD, but not THC or other congeneric cannabinoids tested, potently block replication lung epithelial cells. acts after viral entry, inhibiting gene expression reversing many effects on host transcription. part by up-regulating IRE1α RNase endoplasmic reticulum (ER) stress response interferon signaling...
Stable, effective, easy-to-manufacture vaccines are critical to stopping the COVID-19 pandemic resulting from coronavirus SARS-CoV-2. We constructed a vaccine candidate CoV-RBD121-NP, which is comprised of SARS-CoV-2 receptor-binding domain (RBD) spike glycoprotein (S) fused human IgG1 Fc (CoV-RBD121) and conjugated modified tobacco mosaic virus (TMV) nanoparticle. In vitro, CoV-RBD121 bound host receptor ACE2 monoclonal antibody CR3022, neutralizing that blocks S binding ACE2. The...
Significance Transition metals are required for proper cellular function, which renders them critical all life. To restrict bacterial infection, eukaryotic organisms actively sequester these transition metals, a concept referred to as nutritional immunity. Consequently, pathogens have evolved dedicated mechanisms acquire in order colonize the host. During human plague, Yersinia pestis overcomes iron limitation via production of secreted siderophore yersiniabactin. Here, we identify an...
Serological assays intended for diagnosis, sero-epidemiologic assessment, and measurement of protective antibody titers upon infection or vaccination are essential managing the SARS-CoV-2 pandemic. measuring responses against antigens readily available. However, some lack appropriate characteristics to accurately measure antibodies neutralization. We developed an Enzyme-linked Immunosorbent Assay (ELISA) methods IgG, IgA, IgM SARS-CoV-2, Spike (S), receptor binding domain (RBD), nucleocapsid...
We developed a SARS-CoV-2 vaccine candidate (CoV-RBD121-NP) comprised of tobacco mosaic virus-like nanoparticle conjugated to the receptor-binding domain spike glycoprotein fused human IgG1 Fc domain. CoV-RBD121-NP elicits strong antibody responses in C57BL/6 mice and is stable for up 12 months at 2-8 or 22-28 °C. Here, we showed that this induces neutralizing response K18-hACE2 mice. Furthermore, demonstrated immunization protects from virus-associated mortality symptomatic disease. Our...
Fleas transmit Yersinia pestis directly within the dermis of mammals to cause bubonic plague. Syringe-mediated inoculation is widely used recapitulate plague and study Y. pathogenesis. However, intradermal needle tedious, error prone, poses a significant safety risk for laboratorians. Microneedle arrays (MNAs) are micron-scale polymeric structures that deliver materials dermis, while minimizing sticks. We demonstrated MNA viable strategy bacterial virulence by defining parameters needed...
Abstract Since entering the world stage in December of 2019, SARS-CoV-2 has impacted every corner globe with over 1.48 million deaths and caused untold economic damage. Infections humans range from asymptomatic to severe disease associated dysregulation immune system leading development acute respiratory distress syndrome (ARDs). The distinct shift peripheral monocyte activation infiltration these cells into tract ARDs patients suggests COVID-19 may largely result damage epithelia by...
Abstract Background The distinct shift in peripheral monocyte activation and infiltration of these cells into the respiratory tract observed severe cases COVID-19 suggests that like SARS-CoV-1, acute distress syndrome (ARDs) SARS-CoV-2 infections may result from damage to epithelia by improperly activated macrophages (MPs). In this study, we examined ability non-neutralizing antibodies sensitize MPs killing SARS-CoV-2, as well impact on downstream epithelial cells. Methods Raw 264.7 were...