A5069645784- Skin and Cellular Biology Research
- Cellular Mechanics and Interactions
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Plant Surface Properties and Treatments
- Wound Healing and Treatments
- Hippo pathway signaling and YAP/TAZ
- Renal and related cancers
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Glioma Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Developmental Biology and Gene Regulation
- Cell Adhesion Molecules Research
- Hedgehog Signaling Pathway Studies
Massachusetts General Hospital
2024-2025
King's College London
2019-2022
Guy's Hospital
2019-2021
Cell Therapy Catapult
2019-2021
In human skin the junction between epidermis and dermis undulates, width depth of undulations varying with age disease. When primary epidermal keratinocytes are seeded on collagen-coated polydimethylsiloxane (PDMS) elastomer substrates that mimic epidermal-dermal interface, stem cells become patterned by 24 h, resembling their organisation in living skin. We found cell density nuclear height were higher at base than tips PDMS features. Cells not only expressed levels marker β1 integrin but...
We have examined the developmental origins of Ng2+ perivascular cell populations that adhere to basement membrane blood vessels, and their contribution wound healing. Neural/glial antigen 2 (Ng2) labeled most cells (70–80%) in developing adult mouse back skin, a higher proportion than expressed by other pericyte markers Tbx18, Nestin Pdgfrβ. In skin could be categorized into 4 based on whether they Pdgfrα Pdgfrβ individually or combination were Pdgfr-negative. Lineage tracing demonstrated...
Abstract Group 3 Medulloblastoma (G3MB) is a pediatric brain cancer with poor prognosis, and median 5-year overall survival of 45-58% in infants young children, respectively. Moreover, the current standard-of-care therapy – which includes spinal radiation causes tremendous morbidity. While immunotherapy immune checkpoint blockade has transformed treatment many malignancies, it yet to show benefit most common form tumors. Here we that B cell depletion using Rituximab; anti-CD20: -- an...
Inactivating mutations in the EGF-like ligand binding domain of NOTCH1 are a prominent feature mutational landscape oral squamous cell carcinoma (OSCC). In this study, we investigated keratinocyte lines derived from OSCC biopsies that had been subjected to whole exome sequencing. One line, SJG6, was found have truncating both alleles, resulting loss expression. Overexpression intracellular (NICD) SJG6 cells promoted adhesion and differentiation, while suppressing proliferation, migration...
Quantitative analysis of human induced pluripotent stem cell (iPSC) lines from healthy donors is a powerful tool for uncovering the relationship between genetic variants and cellular behavior. We previously identified rare, deleterious non-synonymous single nucleotide (nsSNVs) in adhesion genes that are associated with outlier iPSC phenotypes state. Here, we generated micropatterned colonies iPSCs to test whether nsSNVs influence patterning radially ordered germ layers. Using custom-built...
Abstract Introduction: Chimeric antigen receptor (CAR)-T cell therapy has revolutionized the treatment of hematological malignancies, but its efficacy in patients with glioblastoma (GBM) and other solid tumors is limited due to poor tumor infiltration an immunosuppressive microenvironment (TME). We have previously shown that blocking vascular endothelial growth factor (VEGF) signaling can normalize vessels, convert TME into immunostimulatory environment, enhance immunotherapy. This study...