Hemoglobin at 1 microM reduced and 10 abolished the endothelium-dependent relaxation induced by acetylcholine or A23187 in rabbit aortic rings. Similarly, methylene blue 50 A23187. Furthermore, hemoglobin (1-10 microM) (10-50 each a dose-dependent inhibition of endothelium-independent produced glyceryl trinitrate, but neither had any effect on isoproterenol. The inhibitory effects may be due to blockade guanylate cyclase, as rises cyclic GMP content which accompany acetylcholine, trinitrate...

Removal of endothelial cells on rings rat aorta increased the sensitivity to selective alpha-1 adrenoceptor agonist phenylephrine, nonselective alpha norepinephrine and alpha-2 clonidine. In case first two, which are strong agonists for adrenoceptor-mediating contraction, removal endothelium 4- 6-fold at EC30 level, but produced little or no increase in maximum. clonidine, a partial adrenoceptor, gave only about 15% maximum given by phenylephrine endothelium-containing rings, not shifted...

<h3>Objective</h3> To determine whether or not radiofrequency ablation (RFA) for persistent atrial fibrillation in patients with advanced heart failure leads to improvements cardiac function. <h3>Setting</h3> Patients were recruited from outpatient clinics Scotland. <h3>Design and intervention</h3> severe left ventricular dysfunction randomised RFA (rhythm control) continued medical treatment (rate control). followed up a minimum of 6 months. <h3>Main outcome measure</h3> Change ejection...

The minimal inhibitory concentrations of 601 clinical isolates anaerobic bacteria to 10 different antimicrobial agents were determined by an agar-dilution technique. Nearly all strains resistant kanamycin and gentamicin, although moderate activity both drugs was noted with Fusobacterium sp., cocci, some Bacteroides melaninogenicus , nonsporeforming gram-positive bacilli. Chloramphenicol at 12.5 μg/ml inhibited but three the tested. Tetracycline 6.25 had high against groups tested, exception...

The effect of the competitive inhibitor L-arginine, NG-monomethyl L-arginine (L-NMMA) on response rat anococcygeus muscle to non-adrenergic, non-cholinergic (NANC) inhibitory nerve stimulation has been examined. L-NMMA causes a rise in tone and inhibition stimulation. stereoisomer D-NMMA is without effect. reversed by L-arginine. Another analogue, L-canavanine, which effective against utilization macrophage, was anococcygeus. These results provide indirect evidence for nitric oxide (NO) or...

This study applied the structured observation technique developed by Mintzberg to high school principalship. Observation of activities performed five principals resulted in a characterization administration that paralleled private sector management many respects. Principals exercised building-level authority over such matters as organi zational maintainance, instructional program, pupil control, and extra-curricular activities. They spent comparatively little time on affairs external...

Neutrophil-mediated injury to lung parenchymal cells has been proposed as an important step in the pathogenesis of many acute and chronic disorders. As vitro model off neutrophil-mediated injury, this study used activated human neutrophils effector 18-h cytotoxicity assay with 51Cr-labeled bovine pulmonary artery endothelial serving target cells. Neutrophils effectively injured cells, expressed cytotoxic index (Cl), 63.8 ± 5.4, could be significantly reduced by several agents, including 1%...

Exposure to indoor air pollution from household burning and solid fuels affects nearly half of the world's population.

The selective cyclic GMP phosphodiesterase inhibitor M&B 22948 and the less inhibitors papaverine isobutylmethylxanthine (IBMX) each induced a component of relaxation rat aortic rings that was endothelium-dependent. most agent at inducing endothelium-dependent 22948, which caused little endothelium-denuded concentrations produced almost complete endothelium-containing rings. Although components by IBMX were clearly present, they well separated from endothelium-independent relaxation. In...

Bradykinin, adenosine triphosphate (ATP) and acetylcholine each relaxed histamine‐contracted strips of pig aorta in a dose‐dependent manner. These relaxations were abolished when the endothelium was removed. Relaxation induced by ATP mimicked diphosphate (ADP) but monophosphate (AMP) about 120 times less potent. antagonized atropine competitive manner, carbachol same degree relaxation as acetylcholine, 10 The calcium ionophore, A23187, also aortic provided present, suggesting that rise level...

We have reported previously that hemoglobin inhibits endothelium-dependent and glyceryl trinitrate-induced relaxation in the rabbit aorta. In this study we examined effects of other ferrous ferric hemoproteins on to determine whether they also share inhibitory properties hemoglobin. Of two tested, myoglobin (1-10 microM) abolished induced by acetylcholine produced a concentration-dependent reduction endothelium-independent trinitrate, manner similar for hemoglobin, but reduced cytochrome C...

Pneumocystis carinii is an extracellular organism which thought to require attachment alveolar epithelial cells for its growth and replication in humans. Fibronectin (Fn) binding P. essential optimal attachment. This study demonstrates that gp120, a 110-120-kD membrane glycoprotein on carinii, mediates of the cultured lung site Fn carinii. A 51Cr-labeled assay was used quantify cell line A549. Addition free purified from whole organisms, caused significant decrease A549 44.2 +/- 5.5% 22.4...

Two directly‐acting stimulants of soluble guanylate cyclase, glyceryl trinitrate (0.1 μ m ) and sodium azide (10 ), a receptor‐mediated stimulant particulate atriopeptin II n each elevated the cyclic GMP content primary cultures pig aortic endothelial cells without affecting AMP content. adenylate glucagon (1 isoprenaline had no effect on or these cells, but directly acting stimulant, forskolin (30 induced small increase in Three agents that release endothelium‐derived relaxing factor...

An inexpensive but practical and simple method is described for providing an environment suitable the maintenance survival of clinically significant anaerobic bacteria, consistent with needs a large-volume laboratory. This modification GasPak procedure based on two concepts. One involved storage “freshly prepared” plating medium under constant stream carbon dioxide. The other concept was to use anaerobe jar, continuously flushed oxygen-free dioxide, as “holding receptacle” inoculated media...

Pulmonary metastasis from hypernephroma usually consists of annular masses scattered throughout both lungs. These rarely give rise to pulmonary symptoms, and unless there is extension the Fig. 1.—Primary hypernephroma. pleura, with development pleural effusion, they seldom produce signs that can be detected by general examination. Extension a tumor this type into lumen bronchus, production bronchostenosis other symptoms simulating primary bronchial carcinoma, sufficiently rare warrant...

In this study we compared the ability of superoxide anion to destroy relaxant activity basal and acetylcholine (ACh)‐stimulated NO in isolated rings rat aorta. Superoxide dismutase (SOD, 1–300 u ml −1 ) induced a concentration‐dependent relaxation phenylephrine (PE)‐induced tone endothelium‐containing which was blocked by N G –nitro‐L‐arginine (l‐NOARG, 30 μ m ), but had no effect on endothelium‐denuded rings. It likely therefore that action SOD resulted from protection basally produced...