A5025008613- Virology and Viral Diseases
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Microbial infections and disease research
- Herpesvirus Infections and Treatments
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Rabies epidemiology and control
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Toxoplasma gondii Research Studies
- Bacterial Identification and Susceptibility Testing
- Respiratory viral infections research
- Molecular Biology Techniques and Applications
- Mycobacterium research and diagnosis
- Antimicrobial Resistance in Staphylococcus
- Aquaculture disease management and microbiota
- Plant Virus Research Studies
- SARS-CoV-2 detection and testing
- Zoonotic diseases and public health
- Bacteriophages and microbial interactions
Purdue University West Lafayette
2019-2025
University of Georgia
2006-2024
Georgia College & State University
2017-2020
University of Sadat City
2014-2020
Mississippi State University
2020
University of Pennsylvania
2020
South University
2020
University of New Hampshire
2019-2020
Chicago Zoological Society
2020
University of Illinois Chicago
2020
Transmission is an essential process that contributes to the survival of pathogens. Ranaviruses are known infect different classes lower vertebrates including amphibians, fishes and reptiles. Differences in likelihood infection among ectothermic vertebrate hosts could explain successful yearlong persistence ranaviruses aquatic environments. The goal this study was determine if transmission a Frog Virus 3 (FV3)-like ranavirus possible three species from classes: Cope’s gray treefrog (Hyla...
Recent outbreaks of canine distemper have prompted examination strains from clinical samples submitted to the University Tennessee College Veterinary Medicine (UTCVM) Clinical Virology Lab. We previously described a new strain CDV that significantly diverged all genotypes reported date including America 2, genotype proposed be main lineage currently circulating in US. The aim this study was determine when appeared and how widespread it is animal populations, given has also been detected...
Abstract Objective —To determine the methicillin-resistant profile of staphylococcal isolates from skin dogs with pyoderma. Animals —90 Procedure —Staphylococci isolated pyoderma were tested for susceptibility to methicillin by use a standard disk diffusion test oxacillin disks. The DNA extracted was mecA gene that encodes penicillinbinding protein 2a (PBP2a) polymerase chain reaction (PCR) assay. expression PBP2a determined commercial latex agglutination Species identified morphologic,...
Abstract Objective —To determine frequency with which Staphylococcus schleiferi could be isolated from dogs pyoderma and antimicrobial susceptibility patterns of isolates that were obtained. Design —Prospective study. Animals —54 a first (n = 14) or recurrent (40) episode pyoderma. Procedure —Specimens obtained submitted for bacterial culture. Isolates identified as S on the basis growth biochemical characteristics. Two DNA sequencing to confirm identification. Methicillin was determined by...
Abstract Ranaviruses have been associated with mortality of lower vertebrates around the world. Frog virus 3 (FV3)-like ranaviruses isolated from different ectothermic vertebrate classes; however, few studies demonstrated whether this pathogen can be transmitted among classes. Using FV3-like American bullfrog Lithobates catesbeianus, eastern box turtle Terrapene carolina carolina, and Pallid Sturgeon Scaphirhynchus albus, we tested for occurrence interclass transmission (i.e., infection)...
There is no gold standard for detection of Rotavirus Group A (RVA), one the main causes diarrhea in neonatal animals. Sensitive and specific real-time RT-PCR (rtRT-PCR) assays are available RVA but require submission clinical samples to diagnostic laboratories. Patient-side immunoassays protein have shown variable results, particularly with from unintended species. sensitive test on farm would facilitate rapid management decisions. The insulated isothermal (RT-iiPCR) assay works a portable...
Canine parvovirus type 2 (CPV-2), including subtypes 2a, 2b and 2c, causes an acute enteric disease in both domestic wild animals. Rapid sensitive diagnosis aids effective management at points of need (PON). A commercially available, field-deployable user-friendly system, designed with insulated isothermal PCR (iiPCR) technology, displays excellent sensitivity specificity for nucleic acid detection. An iiPCR method was developed on-site detection all circulating CPV-2 strains. Limit...
Canine distemper virus (CDV) is a common cause of multisystemic disease in both domestic dogs and wildlife species, including raccoons foxes. Outbreaks CDV eastern Tennessee have occurred since 2012, it was determined that these outbreaks resulted from novel genotype CDV. We hypothesized this also infecting area may be source the for dogs. From 2013 to 2014, autopsies were performed tissues collected (Procyon lotor; n = 50) gray foxes (Urocyon cinereoargenteus; 8) testing. A real-time...
Canine distemper virus (CDV) has been associated with outbreaks of canine infectious respiratory disease in shelters and boarding kennel environments. POCKITTM Nucleic Acid Analyzer is a field-deployable device capable generating automatically interpreted insulated isothermal polymerase chain reaction (iiPCR) results from extracted nucleic acid within one hour. In this study, reverse transcription iiPCR (RT-iiPCR) was developed to facilitate point-of-need diagnosis CDV infection.Analytical...
Antimicrobial resistance (AMR) of bacterial pathogens is an emerging public health threat. This threat extends to pets as it also compromises our ability treat their infections. Surveillance programs in the United States have traditionally focused on collecting data from food animals, foods, and people. The Veterinary Laboratory Investigation Response Network (Vet-LIRN), a national network 45 veterinary diagnostic laboratories, tested antimicrobial susceptibility clinically relevant isolates...
The laboratory diagnosis of infectious diseases, especially those caused by mixed infections, is challenging. Routinely, it requires submission multiple samples to separate laboratories. Advances in next-generation sequencing (NGS) have provided the opportunity for development a comprehensive method identify agents. This study describes use target-specific primers PCR-mediated amplification with NGS technology which pathogen genomic regions interest are enriched and selectively sequenced...
Canine distemper (CD) is a highly contagious, systemic, viral disease of dogs seen worldwide. Despite intensive vaccination in developed countries, recent reports suggest both the re-emergence and increased activity virus (CDV) worldwide, including United States. CDV an RNA genus Morbillivirus within family Paramyxoviridae. Viral genomic encodes six structural proteins. Of proteins, hemagglutinin (H) gene has greatest genetic variation therefore suitable target for molecular epidemiological...
Canine distemper virus (CDV) has caused clinical disease and death in nondomestic felids both captive settings the wild. Outbreaks resulting high mortality rates tigers (Panthera tigris) have prompted some zoos to vaccinate for CDV. In this study, six received a recombinant canarypox-vectored CDV vaccine (1 ml s.c.) were revaccinated with 3 s.c. (mean) 39 days later. Blood collection antibody detection via serum neutralization was performed on 0, 26, 66 post–initial vaccination. No had...
Canine distemper virus (CDV) remains a common cause of infectious disease in dogs, particularly high-density housing situations such as shelters. Vaccination all dogs against CDV is recommended at the time admission to animal shelters and many use modified live (MLV) vaccine. From diagnostic standpoint for with suspected infection, this problematic because highly sensitive real-time reverse transcription polymerase chain reaction (RT-PCR) tests are able detect MLV clinical samples. Real-time...
Although hemoplasma infection has been widely described in animals, a few studies have conducted involving human populations, mostly as case reports. This was cross-sectional epidemiological study that accessed individuals and dogs from ten Indigenous communities of southern southeastern Brazil. A total 23/644 (3.6%) tested positive to hemoplasmas by quantitative polymerase chain reaction (qPCR) (cycle threshold; Ct ≤ 34.4), with 3/644 (0.5%) Mycoplasma haemocanis detection. In addition,...
Objectives The objective of this study was to retrospectively assess the pathogens associated with feline ocular and respiratory diseases in routine diagnostic samples submitted Georgia Veterinary Diagnostic Laboratories. Furthermore, detected by PCR panel upper vs lower tract were compared (specimen separation at pharynx). Methods Test records from panels collected 2012 2022. While targets herpesvirus-1 (FHV), calicivirus (FCV) Chlamydia felis , covers FHV, FCV, C Mycoplasma species,...
Hemoplasmas (hemotropic mycoplasmas) are obligatory red blood cell bacteria that may infect and cause anemia in several mammalian species. IAccordingly, the present study assessed four quilombola communities during six on-field expeditions between December 2021 March 2022 central-eastern Paraná State, southern Brazil. Overall, 12/208 (5.8 %) individuals were positive for hemoplasma infection by qPCR. Results of 16S ribosomal RNA gene sequencing confirmed Mycoplasma heamocanis two human...
Abstract Objective —To measure antibody titers against bovine coronavirus (BCV), determine frequency of BCV in nasal swab specimens, and compare calves treated for respiratory tract disease (BRD) between those given an intranasally administered vaccine control calves. Design —Randomized clinical trial. Animals —414 heifer Procedure —Intranasal antigen concentration titer were measured on entry to a feedlot. Calves randomly assigned receive 3.0 mL modified-live virus enteric rotavirus or...