A5062771455- Protein Structure and Dynamics
- Enzyme Structure and Function
- Mass Spectrometry Techniques and Applications
- Human Mobility and Location-Based Analysis
- Impact of Technology on Adolescents
- Ion channel regulation and function
- Urban Transport and Accessibility
- Protein Interaction Studies and Fluorescence Analysis
- RNA and protein synthesis mechanisms
- Work-Family Balance Challenges
- Alzheimer's disease research and treatments
- Social Media and Politics
- Protein Kinase Regulation and GTPase Signaling
- Photosynthetic Processes and Mechanisms
- Transportation and Mobility Innovations
- Social and Educational Sciences
- Computational Drug Discovery Methods
- Trace Elements in Health
- Spectroscopy and Quantum Chemical Studies
- S100 Proteins and Annexins
- Technology Use by Older Adults
- Migration and Labor Dynamics
- Health disparities and outcomes
- Signaling Pathways in Disease
- Force Microscopy Techniques and Applications
University of Gothenburg
2013-2025
Lund University
2008-2020
University College Dublin
2007-2008
University of Helsinki
2005
Haverford College
2004
Aarhus University
2002
National Institute of Chemical Physics and Biophysics
2001
DuPont (United States)
1996
Rutgers, The State University of New Jersey
1996
Wilmington University
1996
Due to their small size, nanoparticles have distinct properties compared with the bulk form of same materials. These are rapidly revolutionizing many areas medicine and technology. Despite remarkable speed development nanoscience, relatively little is known about interaction nanoscale objects living systems. In a biological fluid, proteins associate nanoparticles, amount presentation on surface particles leads an in vivo response. Proteins compete for nanoparticle "surface," leading protein...
Nanoparticles present enormous surface areas and are found to enhance the rate of protein fibrillation by decreasing lag time for nucleation. Protein is involved in many human diseases, including Alzheimer's, Creutzfeld-Jacob disease, dialysis-related amyloidosis. Fibril formation occurs nucleation-dependent kinetics, wherein a critical nucleus key rate-determining step, after which proceeds rapidly. We show that nanoparticles (copolymer particles, cerium oxide quantum dots, carbon...
Copolymeric NiPAM:BAM nanoparticles of varying hydrophobicity were found to retard fibrillation the Alzheimer's disease-associated amyloid β protein (Aβ). We that these affect mainly nucleation step Aβ fibrillation. The elongation is largely unaffected by particles, and once nucleated, process occurs with same rate as in absence nanoparticles. extension lag phase for strongly dependent on both amount surface character Surface plasmon resonance studies show binds provide equilibrium constants...
Nanoparticles in biological fluids almost invariably become coated with proteins that may confer nanomedical and nanotoxicological effects. Understanding these effects requires quantitative measurements using simple systems. Adsorption of HSA to copolymer nanoparticles varying hydrophobicity curvature was studied ITC, yielding stoichiometry, affinity, enthalpy changes upon binding. The controlled via the co-monomer ratio, N-iso-propylacrylamide/N-tert-butylacrylamide. most hydrophobic...
This study investigates the increased adoption of telework in Sweden between 2005 and 2012. It uses microlevel data from national surveys order to ask where is being adopted by whom. Results indicate that has become routine for over 20 per cent all gainfully employed. Expansion explained a working life transition: besides enabling information communication technologies, factors associate with managers’ trust control; character jobs, work tasks contracts knowledge‐based industries; individual...
This study explores how changing conditions for home-based telework affect the quality of life and social sustainability workers in terms time pressure use control everyday life. Changing concern spread to new types jobs a more routine character, involving practices unregulated work anytime smartphone access. Empirically, we draw on survey data from sample 456 teleworkers employed by six governmental agencies Sweden. Results indicate that subjective is not associated with job type...
We report the development of a high-level bacterial expression system for Alzheimer's disease-associated amyloid beta-peptide (Abeta), together with scaleable and inexpensive purification procedure. Abeta(1-40) Abeta(1-42) coding sequences added ATG codons were cloned directly into Pet vector to facilitate production Met-Abeta(1-40) Met-Abeta(1-42), referred as Abeta(M1-40) Abeta(M1-42), respectively. The designed using preferred by Escherichia coli, two peptides expressed in this host...
The aggregation of the amyloid-β (Aβ) peptide is linked to pathogenesis Alzheimer's disease (AD). In particular, some point mutations within Aβ are associated with early-onset familial disease. Here we set out explore how physical properties altered side chains, including their sizes and charges, affect molecular mechanisms aggregation. We focus on Aβ42 mutations-A21G (Flemish), E22K (Italian), E22G (Arctic), E22Q (Dutch), D23N (Iowa)-which lead similar or identical pathology sporadic AD...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure-function relationships in EF-hand calcium-binding proteins. Protein engineering and biophysical studies of calbindin D9kSara Linse, Peter Brodin, Torbjoern Drakenberg, Eva Thulin, Sellers, Karin Elmden, Thomas Grundstroem, Sture ForsenCite this: Biochemistry 1987, 26, 21, 6723–6735Publication Date (Print):October 1, 1987Publication History Published online1 May 2002Published inissue 1 October 1987https://doi.org/10.1021/bi00395a023RIGHTS...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMetal ion and drug binding to proteolytic fragments of calmodulin: cadmium-113 proton nuclear magnetic resonance studiesEva Thulin, Anita Andersson, Torbjoern Drakenberg, Sture Forsen, Hans J. VogelCite this: Biochemistry 1984, 23, 8, 1862–1870Publication Date (Print):April 10, 1984Publication History Published online1 May 2002Published inissue 10 April...
A complete analysis of calbindin D9k by two-dimensional 1H nuclear magnetic resonance spectroscopy has established the existence two conformations for folded protein in solution. Well-resolved major and minor resonances a ratio 3:1 are observed throughout NMR spectrum. Two-dimensional exchange experiments show that species related an equilibrium process. Analysis short proton-proton distances along peptide backbone, identified Overhauser effect spectroscopy, provides unambiguous evidence...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCadmium-113 nuclear magnetic resonance studies of proteolytic fragments calmodulin: assignment strong and weak cation binding sitesAnita Andersson, Sture Forsen, Eva Thulin, Jans J. VogelCite this: Biochemistry 1983, 22, 10, 2309–2313Publication Date (Print):May 1983Publication History Published online1 May 2002Published inissue 10 1983https://pubs.acs.org/doi/10.1021/bi00279a001https://doi.org/10.1021/bi00279a001research-articleACS...
Aggregation of the amyloid β-protein (Aβ) is believed to be involved in Alzheimer's disease pathogenesis. Here we have investigated importance aromatic rings at positions 19 and 20 for aggregation rate mechanism by substituting phenylalanine with leucine. kinetics were monitored as a function time peptide concentration thioflavin T (ThT) fluorescence, equilibrium sedimentation assay, structural changes using circular dichroism spectroscopy presence fibrillar material was detected...
1 Monomeric -chains were conjugated with CNBr-activated Sepharose 4B. The C-terminal cysteine of the -chain was converted to a mixed disulfide 3-carboxy-4-nitro-benzenethiol (Nbs) and used separate plasma proteins reactive thiol groups. protiens, α1-antitrypsin prealbumin have greatest affinity for interchange reaction disulfides. link between is sensitive excess Nbs, selectively cleaved in presence 5,5-dithiobis(2-nitrobenzoate) (Nbs2) which accepts sulfhydryl group α1-antitrypsin. 2 A...
We explore from a time-use perspective how private use of computers and the Internet [information communications technologies (ICTs)] is transforming everyday life. Data Swedish 2010–2011 Time-Use Survey reflect situation in which has spread widely become routine for many. Using covariate analysis, we analyse differences general time between four groups ICT users ranging non-users to heavy users. The theoretical departure point nuanced discussion time-displacement concept. Results indicate...
This article contributes to the geographical understanding of how mobile online presence enabled by smartphones transforms human spatial practices; that is, people’s everyday routines and experiences in time space. Contrasting a mainstream discourse concentrating on autonomy flexibility ubiquitous (anywhere, anytime) use social media, we examine new mounting constraints user agency. Building time-geographic theory, advance novel insights into virtualities young lives they are materialized...
Mortality among people with human immunodeficiency virus (HIV) declined the introduction of combination antiretroviral therapy. We investigated trends in mortality HIV from 1999 through 2020. Data were collected Collection on Adverse events Anti-HIV Drugs (D:A:D) cohort between January 2015 and International Cohort Consortium Infectious Disease (RESPOND) October 2017 December Age-standardized all-cause cause-specific rates, classified using Coding Causes Death HIV, calculated. Poisson models...
Calbindin D<sub>28k</sub> is a member of the calmodulin superfamily Ca<sup>2+</sup>-binding proteins and contains six EF-hands. The protein generally believed to function as Ca<sup>2+</sup> buffer, but studies presented in this work indicate that it may also act sensor. results show Mg<sup>2+</sup> binds same sites Ca<sup>2+</sup>with an association constant ∼1.4·10<sup>3</sup>m<sup>−1</sup> 0.15 m KCl. four high affinity calbindin bind non-sequential, parallel manner. In presence...
Abstract EF‐hand Ca 2+ ‐binding proteins participate in both modulation of signals and direct transduction the ionic signal into downstream biochemical events. The range functions these is correlated with differences way which they respond to binding . domains calbindin D 9k calmodulin are homologous, yet calcium ions a drastically different manner. A series comparative analyses their structures enabled development hypotheses about residues control calcium‐induced changes conformation. To...