A5020544186- Sarcoma Diagnosis and Treatment
- RNA modifications and cancer
- Neuroblastoma Research and Treatments
- Cancer-related molecular mechanisms research
- Tumors and Oncological Cases
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Prostate Cancer Treatment and Research
- Bone Tumor Diagnosis and Treatments
- Virus-based gene therapy research
- Lymphoma Diagnosis and Treatment
- Nanoparticle-Based Drug Delivery
- Protein Degradation and Inhibitors
- RNA Research and Splicing
- Cancer, Lipids, and Metabolism
- Mitochondrial Function and Pathology
- Cancer-related Molecular Pathways
- Cancer therapeutics and mechanisms
- CAR-T cell therapy research
- Nanoplatforms for cancer theranostics
Children's Hospital of Los Angeles
2016-2025
Van Andel Institute
2018-2024
University of Southern California
2015-2024
Michigan State University
2020-2024
Texas Tech University
2010-2024
Texas Tech University Health Sciences Center
2024
Keck Graduate Institute
2023
USC Norris Comprehensive Cancer Center
2018-2021
APLA Health
2005-2020
Duke University
2019
Precise diagnosis of small-round-cell tumors is often a challenge to the pathologist and clinical oncologist. In Ewing's sarcomas related peripheral primitive neuroectodermal tumors, t(11;22) translocation or (21,22) rearrangement associated with hybrid transcripts EWS gene FLI1 ERG gene. To investigate diagnostic implication this observation, we searched for these in from patients radiologic features sarcoma tumors.Samples RNA 114 were reverse transcribed subjected polymerase chain reaction...
The minfi package is widely used for analyzing Illumina DNA methylation array data. Here we describe modifications to the required support HumanMethylationEPIC ('EPIC') from Illumina. We discuss methods joint analysis and normalization of data HumanMethylation450 ('450k') EPIC platforms. introduce single-sample Noob ( ssNoob ) method, a procedure suitable incremental preprocessing individual arrays conclude that this method should be when integrating multiple generations Infinium arrays....
PURPOSE: Alveolar rhabdomyosarcoma (ARMS) is an aggressive soft tissue malignancy of children and adolescents. Most ARMS patients express PAX3-FKHR or PAX7-FKHR gene fusions resulting from t(2;13) t(1;13) translocations, respectively. We wished to confirm the diagnostic specificity fusion detection in a large cohort RMS evaluate whether these alterations influence clinical outcome ARMS. PATIENTS AND METHODS: determined status 171 childhood (RMS) entered onto Intergroup Rhabdomyosarcoma Study...
Purpose Clinicopathologic features and biochemical recurrence are sensitive, but not specific, predictors of metastatic disease lethal prostate cancer. We hypothesize that a genomic expression signature detected in the primary tumor represents true biological potential aggressive provides improved prediction early cancer metastasis. Methods A nested case-control design was used to select 639 patients from Mayo Clinic registry who underwent radical prostatectomy between 1987 2001. classifier...
Abstract The development of effective, systemic therapies for metastatic cancer is highly desired. We show here that the delivery sequence-specific small interfering RNA (siRNA) against EWS-FLI1 gene product by a targeted, nonviral system dramatically inhibits tumor growth in murine model Ewing's sarcoma. uses cyclodextrin-containing polycation to bind and protect siRNA transferrin as targeting ligand receptor–expressing cells. Removal or use control sequence eliminates antitumor effects....
Background. There is a need to develop single prognostically significant classification of rhabdomyosarcomas (RMS) and other related tumors children, adolescents, young adults which would be current guide for their diagnosis, allow valid comparison outcomes between protocols carried out anywhere in the world, should enhance recognition prognostic subsets. Method. Sixteen pathologists from eight pathology groups, representing six countries several cooperative classified by four...
We report a new class of artifacts in DNA methylation measurements from Illumina HumanMethylation450 and MethylationEPIC arrays. These reflect failed hybridization to target DNA, often due germline or somatic deletions manifest as incorrectly reported intermediate methylation. The survive existing preprocessing pipelines, masquerade epigenetic alterations can confound discoveries epigenome-wide association studies methylation-quantitative trait loci. implement solution, P-value with...
PERIPHERAL neuroepithelioma (peripheral neuroblastoma) is an uncommon malignant tumor of the peripheral nervous system with a histologic appearance similar to that classical childhood neuroblastoma.1 2 3 In contrast neuroblastoma, it typically presents in older children and young adults, can be associated nerves, spares adrenal glands sympathetic ganglia.We have studied both specimens tumor-cell lines from two patients neuroepithelioma; each case, we found these karyotypes include...
The Ewing sarcoma family of tumors (EFT) is a group highly malignant small round blue cell occurring in children and young adults. We report here the largest genomic survey to date 101 EFT (65 36 lines). Using combination whole genome sequencing targeted approaches, we discover that has very low mutational burden (0.15 mutations/Mb) but frequent deleterious mutations cohesin complex subunit STAG2 (21.5% tumors, 44.4% lines), homozygous deletion CDKN2A (13.8% 50%) TP53 (6.2% 71.9%)....
One hundred twenty-four children and young adults with recurrent tumors, predominantly sarcomas, were treated the combination of ifosfamide, etoposide, uroprotector, mesna (2-mercaptoethane sulphonate), in a phase II trial. The treatment regimen consisted 12 cycles therapy administered every 3 weeks. After evaluation tumor response to chemotherapy alone, radiation or surgery was used eradicate residual sites metastatic disease where possible. At present time, 77 patients are evaluable for...
No AccessJournal of UrologyAdult Urology1 Dec 2013Validation a Genomic Classifier that Predicts Metastasis Following Radical Prostatectomy in an At Risk Patient Population R. Jeffrey Karnes, Eric J. Bergstralh, Elai Davicioni, Mercedeh Ghadessi, Christine Buerki, Anirban P. Mitra, Anamaria Crisan, Nicholas Erho, Ismael A. Vergara, Lucia L. Lam, Rachel Carlson, Darby J.S. Thompson, Zaid Haddad, Benedikt Zimmermann, Thomas Sierocinski, Timothy Triche, Kollmeyer, Karla V. Ballman, Peter C....
Rhabdomyosarcoma (RMS) in children occurs as two major histological subtypes, embryonal (ERMS) and alveolar (ARMS). ERMS is associated with an 11p15.5 loss of heterozygosity (LOH) may be confused nonmyogenic, non-RMS soft tissue sarcomas. ARMS expresses the product a genomic translocation that fuses FOXO1 (FKHR) either PAX3 or PAX7 (P-F); however, at least 25% cases lack these translocations. Here, we describe genomic-based classification scheme derived from combined gene expression...
Discovery that the transcriptional output of human genome is far more complex than predicted by current set protein-coding annotations and most RNAs produced do not appear to encode proteins has transformed our understanding complexity suggests new paradigms regulation. However, fraction all cellular RNA whose function we understand utilized produce remain controversial. This simply a bookkeeping issue because degree which this un-annotated transcription present important implications with...
Background Rhabdomyosarcoma (RMS) is divided into two major histological subtypes: alveolar (ARMS) and embryonal (ERMS), with most ARMS expressing one of oncogenic genes fusing PAX3 or PAX7 FOXO1 (P3F P7F, respectively). The Children's Oncology Group (COG) carried out a multi-institutional clinical trial to evaluate the prognostic value PAX-FOXO1 fusion status. Methods Study participants were treated on COG protocol D9803 for intermediate risk ERMS using multi-agent chemotherapy,...
The recent COVID-19 pandemic is a treatment challenge in the acute infection stage but recognition of chronic symptoms termed post-acute sequelae SARS-CoV-2 (PASC) may affect up to 30% all infected individuals. underlying mechanism and source this distinct immunologic condition three months or more after initial remains elusive. Here, we investigated presence S1 protein 46 We analyzed T-cell, B-cell, monocytic subsets both severe patients with (PASC). levels intermediate (CD14+, CD16+)...
Alveolar rhabdomyosarcomas (ARMS) are aggressive soft-tissue sarcomas affecting children and young adults. Most ARMS tumors express the PAX3-FKHR or PAX7-FKHR (PAX-FKHR) fusion genes resulting from t(2;13) t(1;13) chromosomal translocations, respectively. However, up to 25% of negative, making it unclear whether represent a single disease multiple clinical biological entities with common phenotype. To test what extent PAX-FKHR determine class behavior ARMS, we used oligonucleotide microarray...
Pulmonary metastasis continues to be the most common cause of death in osteosarcoma. Indeed, 5-year survival for newly diagnosed osteosarcoma patients has not significantly changed over 20 years. Further understanding mechanisms and resistance this aggressive pediatric cancer is necessary. Pet dogs naturally develop providing a novel opportunity model development progression. Given accelerated biology canine osteosarcoma, we hypothesized that direct comparison expression profiles may help...