A5055765751- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Muscle activation and electromyography studies
- Planarian Biology and Electrostimulation
- Genetics and Physical Performance
- Erythrocyte Function and Pathophysiology
- Pain Mechanisms and Treatments
- Transcranial Magnetic Stimulation Studies
- Molecular Communication and Nanonetworks
- Sports Performance and Training
- Photoreceptor and optogenetics research
- Adipose Tissue and Metabolism
San Jose State University
2020-2025
Animals requiring purposeful movement for survival are endowed with mechanoreceptors, called proprioceptors, that provide essential sensory feedback from muscles and joints to spinal cord circuits, which modulates motor output. Despite the nature of proprioceptive signaling in daily life, mechanisms governing proprioceptor activity poorly understood. Here, we identified nonredundant roles two voltage-gated sodium channels (Na V s), Na 1.1 1.6, mammalian proprioception. Deletion 1.6...
The voltage-gated sodium channel (Na V ), Na 1.1, is well-studied in the central nervous system; conversely, its contribution to peripheral sensory neuron function more enigmatic. Here, we identify a new role for 1.1 mammalian proprioception. RNAscope analysis and vitro patch-clamp recordings genetically identified mouse proprioceptors show ubiquitous expression significant contributions intrinsic excitability. Notably, genetic deletion of neurons caused profound visible motor coordination...
Muscle spindle afferents are slowly adapting low threshold mechanoreceptors that report muscle length and movement information critical for motor control proprioception. The rapidly cation channel PIEZO2 has been identified as necessary afferent stretch sensitivity, although the properties of this suggest additional molecular elements mediating complex response afferents. We glutamate increases static sensitivity in an ex vivo mouse nerve preparation, blocking packaging into vesicles by sole...
It is challenging to stimulate gamma motor neurons, which are important regulators of muscle spindle afferent function, without also recruiting alpha neurons. Here, we test the feasibility stimulating neuron axons using optogenetics in two transgenic mouse lines. We used an ex vivo muscle-nerve preparation adult mice monitor firing, should increase response neuron-induced lengthening sensory region spindle. A force transducer measured neuron-mediated twitch contractions. Blue LED light (470...
Voltage gated sodium channels (Na V s) are crucial for the generation and propagation of action potentials. There 9 different isoforms found in mammals 1.1-1.9), Na 1.1, 1.6, 1.7 muscle proprioceptors that sense stretch, spindle afferents. This sensory information is critical motor reflexes body limb position space. We previously mice lacking one or both copies 1.1 peripheral neurons displayed deficits, including intention tremors. Muscle afferents these also had unstable static stretch...
Abstract Animals that require purposeful movement for survival are endowed with mechanosensory neurons called proprioceptors provide essential sensory feedback from muscles and joints to spinal cord circuits, which modulates motor output. Despite the nature of proprioceptive signaling in daily life, mechanisms governing proprioceptor activity poorly understood. Here, we have identified distinct nonredundant roles two voltage-gated sodium channels (NaVs), NaV1.1 NaV1.6, mammalian...
Group Ia and II muscle spindle afferents are sensory neurons critical to detecting movement length, which is important in proprioception motor control. Glutamate released by synaptic‐like vesicles (SLVs) at afferent nerve endings exogenous glutamate increases neuron firing rate during stretch (Bewick, et al., 2005). However, the activity of individual was not measured, allowing for possibility that this increased also included other known have receptors, like nociceptors. Here we test...
The primary sensory input for proprioception, or the sense of body position in space, comes from muscle spindle afferents, which are mechanoreceptors that relay information about changes length. is also innervated by sympathetic neurons, but role this innervation on function not well understood. Here we test hypothesis afferents will exhibit decreased firing response to stretch following exposure neurotransmitters norepinephrine and epinephrine. To used an ex vivo mouse muscle-nerve...
A bstract The voltage-gated sodium channel (Na V ), Na 1.1, is well-studied in the central nervous system; conversely, its contribution to peripheral sensory neuron function more enigmatic. Here, we identify a new role for 1.1 mammalian proprioception. RNAscope analysis and vitro patch clamp recordings genetically identified mouse proprioceptors show ubiquitously expression significant contributions intrinsic excitability. Notably, genetic deletion of neurons caused profound visible motor...
The molecular mechanisms by which Group Ia and II muscle spindle (MS) afferents transduce movement into action potentials is not fully understood, although the rapidly adapting PIEZO2 mechanically sensitive cation channel essential. Mechanosensation in many types of sensory neurons relies on PIEZO2, suggesting that unique response properties are mediated complement mediators found different neurons. Synaptic-like vesicles containing glutamate released from MS afferent receptor ending a...