James M. Pflug

ORCID: 0000-0003-4890-0270
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About
Contact & Profiles
Research Areas
  • Forest Insect Ecology and Management
  • Coleoptera Taxonomy and Distribution
  • Forest Ecology and Biodiversity Studies
  • Coleoptera: Cerambycidae studies
  • Lepidoptera: Biology and Taxonomy
  • Genomics and Phylogenetic Studies
  • Plant Disease Resistance and Genetics
  • Advanced Surface Polishing Techniques
  • Semiconductor materials and interfaces
  • Insect symbiosis and bacterial influences
  • Environmental DNA in Biodiversity Studies
  • Scarabaeidae Beetle Taxonomy and Biogeography
  • Silk-based biomaterials and applications
  • Chromosomal and Genetic Variations

Oregon State University
2015-2024

Measuring genome size across different species can yield important insights into evolution of the and allow for more informed decisions when designing next-generation genomic sequencing projects. New techniques estimating using shallow sequence data have emerged which potential to augment our knowledge sizes, yet these methods only been used in a limited number empirical studies. In this project, we compare estimation (k-mer average read depth single-copy genes) measurements from flow...

10.1534/g3.120.401028 article EN cc-by G3 Genes Genomes Genetics 2020-06-29

In this paper we explore high-throughput Illumina sequencing of nuclear protein-coding, ribosomal, and mitochondrial genes in small, dried insects stored natural history collections. We sequenced one tenebrionid beetle 12 carabid beetles ranging size from 3.7 to 9.7 mm length that have been various museums for 4 84 years. Although chose a number old, small specimens which expected low sequence recovery, successfully recovered at least some low-copy protein-coding all specimens. For example,...

10.1371/journal.pone.0143929 article EN cc-by PLoS ONE 2015-12-30

Abstract Adephaga is the second largest suborder of Coleoptera and contains aquatic terrestrial groups that are sometimes classified as Hydradephaga Geadephaga, respectively. The phylogenetic relationships have been studied intensively, but major subgroups Geadephaga placement Hygrobiidae within Dytiscoidea remain obscure. Here, we infer new DNA‐hybridization baits for exon‐capture phylogenomics combine hybrid‐capture sequence data with transcriptomes to generate phylogenomic taxon sampling...

10.1111/syen.12508 article EN cc-by Systematic Entomology 2021-07-26

Targeted capture and enrichment approaches have proven effective for phylogenetic study. Ultraconserved elements (UCEs) in particular exhibited great utility phylogenomic analyses, with the software package phyluce being among most utilized pipelines UCE phylogenomics, including probe design. Despite success of UCEs, it is becoming increasing apparent that diverse lineages require sets tailored to focal taxa order improve locus recovery. However, factors affecting design methods optimizing...

10.1002/ece3.5260 article EN cc-by Ecology and Evolution 2019-06-01

The phylogeny of the carabid beetle supertribe Nebriitae is inferred from analyses DNA sequence data eight gene fragments including one nuclear ribosomal (28S), four nuclear-protein coding genes (CAD, topoisomerase 1, PEPCK, and wingless), three mitochondrial (16S + tRNA-Leu ND1, COI ("barcode" region) ("Pat/Jer" region)). Our taxon sample included 264 exemplars representing 241 species subspecies (25% known nebriite fauna), 39 41 currently accepted genera subgenera (all except Notiokasis...

10.3897/zookeys.1044.62245 article EN cc-by ZooKeys 2021-06-16

ABSTRACT Measuring genome size across different species can yield important insights into evolution of the and allow for more informed decisions when designing next-generation genomic sequencing projects. New techniques estimating using shallow sequence data have emerged which potential to augment our knowledge sizes, yet these methods only been used in a limited number empirical studies. In this project, we compare estimation (k-mer average read depth single-copy genes) measurements from...

10.1101/761304 preprint EN cc-by bioRxiv (Cold Spring Harbor Laboratory) 2019-09-08

Abstract Bembidion Latreille (Coleoptera: Carabidae) is a genus of small ground beetles containing about 1,380 species. To test previous phylogenetic hypotheses deeper lineages and near relatives, we recover examine over 1,800 nuclear protein-coding loci from 33 species representing the main Bembidion, 10 other bembidiine genera, 7 outgroups. We find that exclusive subgenus Phyla Motschulsky monophyletic, reclassify as separate genus. Within 2 dominant clades, superseries (containing 490 in...

10.1093/isd/ixae025 article EN Insect Systematics and Diversity 2024-09-01

I will present results from our phylogenetic study of the family Carabidae, based on DNA sequences six gene fragments for about 550 carabid species representing 80 tribes, as well transcriptomic data and hybrid capture genomic a representative sampling lineages. Many morphologically delimited groups are confirmed monophyletic, relationships discovered in previous sequence-based studies (e.g., Brachinini is sister to Harpalinae (sensu Erwin 1985), which combined Moriomorphini; Pseudomorphini...

10.3897/aca.2.e37366 article EN ARPHA Conference Abstracts 2019-06-18
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