A5014911603- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Advanced biosensing and bioanalysis techniques
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Genetics, Bioinformatics, and Biomedical Research
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
John Brown University
2025
Target (United States)
2024
Brown University
2019-2024
University of Connecticut
2018
The intuitive manipulation of specific amino acids to alter the activity or specificity CRISPR-Cas9 has been a topic great interest. As large multi-domain RNA-guided endonuclease, intricate molecular crosstalk within Cas9 protein hinges on its conformational dynamics, but comprehensive understanding extent and timescale motions that drive allosteric function association with nucleic remains elusive. Here, we investigated structure multi-timescale recognition (Rec) lobe Geo Cas9, thermophilic...
The intuitive manipulation of specific amino acids to alter the activity or specificity CRISPR-Cas9 has been a topic great interest. As large multi-domain RNA-guided endonuclease, intricate molecular crosstalk within Cas9 protein hinges on its conformational dynamics, but comprehensive understanding extent and timescale motions that drive allosteric function association with nucleic remains elusive. Here, we investigated structure multi-timescale recognition (Rec) lobe Geo Cas9, thermophilic...
The intuitive manipulation of specific amino acids to alter the activity or specificity CRISPR-Cas9 has been a topic great interest. As large multi-domain RNA-guided endonuclease, intricate molecular crosstalk within Cas9 protein hinges on its conformational dynamics, but comprehensive understanding extent and timescale motions that drive allosteric function association with nucleic remains elusive. Here, we investigated structure multi-timescale recognition (Rec) lobe Geo Cas9, thermophilic...
Allosteric signaling within multidomain proteins is a driver of communication between spatially distant functional sites. Understanding the mechanism allosteric coupling in large most promising route to achieving spatial and temporal control system. The recent explosion CRISPR-Cas9 applications molecular biology medicine has created need understand how atomic level protein dynamics Cas9, which are driving force its crosstalk, influence biophysical characteristics. In this study, we used...
The intuitive manipulation of specific amino acids to alter the activity or specificity CRISPR-Cas9 has been a topic great interest. As large multi-domain RNA-guided endonuclease, intricate molecular crosstalk within Cas9 protein hinges on its conformational dynamics, but comprehensive understanding extent and timescale motions that drive allosteric function association with nucleic remains elusive. Here, we investigated structure multi-timescale recognition (Rec) lobe GeoCas9, thermophilic...
The intuitive manipulation of specific amino acids to alter the activity or specificity CRISPR-Cas9 has been a topic great interest. As large multi-domain RNA-guided endonuclease, intricate molecular crosstalk within Cas9 protein hinges on its conformational dynamics, but comprehensive understanding extent and timescale motions that drive allosteric function association with nucleic remains elusive. Here, we investigated structure multi-timescale recognition (Rec) lobe Geo Cas9, thermophilic...
The intuitive manipulation of specific amino acids to alter the activity or specificity CRISPR-Cas9 has been a topic great interest. As large multi-domain RNA-guided endonuclease, intricate molecular crosstalk within Cas9 protein hinges on its conformational dynamics, but comprehensive understanding extent and timescale motions that drive allosteric function association with nucleic remains elusive. Here, we investigated structure multi-timescale recognition (Rec) lobe GeoCas9, thermophilic...
CRISPR‐Cas9 is a widely utilized biochemical tool with applications in biotechnology and medicine. Target DNA recognition nuclease sites of Cas9 are spatially separated yet functionally coupled, indicative an allosteric crosstalk throughout the enzyme. There structural dynamic changes occuring that critical for this signal propagation. However, understanding molecular motions guiding how influences its mechanism unknown. In study we report on properties HNH domain between extensively studied...