A5041363133- Adenosine and Purinergic Signaling
- Immune Cell Function and Interaction
- Neuroscience of respiration and sleep
- Adolescent and Pediatric Healthcare
- Hematopoietic Stem Cell Transplantation
- Mast cells and histamine
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- T-cell and B-cell Immunology
- Neonatal Respiratory Health Research
- Microfluidic and Capillary Electrophoresis Applications
- Bipolar Disorder and Treatment
- Microfluidic and Bio-sensing Technologies
- Pancreatic function and diabetes
- Immunotherapy and Immune Responses
- Calcium signaling and nucleotide metabolism
- Advanced Nanomaterials in Catalysis
- Nanoparticles: synthesis and applications
- Streptococcal Infections and Treatments
- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- Neurological Complications and Syndromes
- Advanced biosensing and bioanalysis techniques
- Neonatal and Maternal Infections
- Urinary Tract Infections Management
University of Wollongong
2016-2025
Sustainable Horizons Institute
2020-2025
Illawarra Health and Medical Research Institute
2015-2024
Creative Commons
2022
RMIT University
2014
The University of Sydney
2000-2010
Nepean Hospital
2001-2010
Royal Prince Alfred Hospital
1996-2001
This paper presents an inertial microfluidic device with a simple serpentine micro-channel to continuously separate particles high performance. Separation of micro/nano-particles has variety potential applications in biomedicine and industry. Among the existing separation technologies, label-free technique without use antibody affinity, filter or centrifugation is highly desired ensure minimal damage alteration cells. Inertial microfluidics utilising hydrodynamic forces one most suitable...
Abstract Since the beginning of human history, demand for effective healthcare systems diagnosis and treatment health problems has grown steadily. However, traditional centralized requires hospital visits, making in‐time long‐term challenging. Bioelectronics shown potential in patient‐friendly owing to rapid advances diverse fields biology electronics. In particular, wearable implantable bioelectronics have emerged as an alternative or adjunct conventional healthcare. To develop into...
The P2X7 receptor is a ligand-gated cation-selective channel that mediates ATP-induced apoptosis of cells the immune system. We and others have shown nonfunctional both in lymphocytes monocytes from some subjects. To study possible genetic basis we sequenced DNA coding for carboxyl-terminal tail P2X7. In 9 45 normal subjects heterozygous nucleotide substitution (1513A→C) was found, whereas 1 subject carried homozygous codes glutamic acid to alanine at amino position 496. Surface expression...
Genetic variation influences susceptibility to clinical tuberculosis (TB). Activation of the P2X(7) receptor on human macrophages induces killing mycobacteria. We have identified polymorphisms in gene that markedly reduce this killing.To determine if P2X7 are associated with increased risk TB, prevalence four was assessed individuals from Southeast Asia, where majority patients TB our study originate. The association these subsequently investigated two separate case-control cohorts and...
The P2X7 receptor is an ATP-gated cation channel expressed in immune cells and plays a role proinflammatory cytokine release from monocytes macrophages. This study investigated the coinheritance of 12 functionally relevant single nucleotide polymorphisms (SNPs) human gene (P2RX7), functional effect each singly combination was assessed by measurements ATP-induced currents ethidium+ uptake. Genotyping 3430 Caucasian subjects identified 4 common haplotypes addition to (wild-type) P2X7-1. Two...
The P2X7 receptor is a ligand-gated cation channel that highly expressed on mononuclear leukocytes and mediates ATP-induced apoptosis killing of intracellular pathogens. There wide variation in function between subjects, explained part by four loss-of-function polymorphisms (R307Q, E496A, I568N, 5′-intronic splice site polymorphism), as well rare mutations. In this study, we report the allele frequencies 11 non-synonymous describe fifth polymorphism gene (1096C → G), which changes Thr357 to...
The P2X7 receptor is a ligand-gated channel that highly expressed on mononuclear cells and mediates ATP-induced apoptosis of these cells. Wide variations in the function have been observed, part because loss-of-function polymorphism changes Glu-496 to Ala without affecting surface expression lymphocytes. In this study second (Ile-568 Asn) has found heterozygous dosage three 85 normal subjects 45 patients with chronic lymphocytic leukemia. was measured by fluxes Rb+, Ba2+, ethidium+ into...
Abstract Priming of monocytes with LPS produces large quantities intracellular, biologically inactive IL-1β that can be processed and released by subsequent activation the P2X7 receptor extracellular ATP. We examined whether a loss-of-function polymorphism human (Glu496Ala) impairs this process. Both ATP-induced ethidium+ uptake shedding L-selectin (CD62L) were nearly absent in from four subjects homozygous for Glu496Ala confirming function. The level 2 h LPS-activated whole blood was 50%...
The P2X7 receptor is a ligand-gated channel that highly expressed on mononuclear cells of the immune system and mediates ATP-induced apoptosis. Wide variations in function P2X have been observed, explained part by 7loss-of-function polymorphisms change Glu496 to Ala (E496A) Ile568 Asn (I568N). In this study, third polymorphism, which substitutes an uncharged glutamine for positively charged Arg307 (R307Q), has found heterozygous dosage 12 420 subjects studied. was measured fluxes Rb+, Ba2+,...
The P2X7 purinergic receptor is a ligand-gated cation channel expressed on leukocytes including microglia. This study aimed to determine if activation induces the uptake of organic cations, reactive oxygen species (ROS) formation, and death in murine microglial EOC13 cell line. Using macrophage J774 line as positive control, RT-PCR, immunoblotting, immunolabelling established presence cells. A cytofluorometric assay demonstrated that agonists adenosine-5′-triphosphate (ATP)...
P2X7 is a ligand-gated ion channel which activated by ATP and displays secondary permeability characteristics. The mechanism of development the pathway currently unclear, although role for hemichannel protein pannexin-1 has been suggested. In this study we investigated in P2X7-induced dye uptake ATP-induced IL-1β secretion from human monocytes. We found no pharmacological evidence involvement P2X7-mediated transfected HEK-293 cells with inhibition seen carbenoxolone mimetic inhibitory...
By exploiting the Dean-flow-coupled elasto-inertial effects, continuous, sheathless, and high purity plasma extraction under viscoelastic fluid in a straight channel with asymmetrical expansion–contraction cavity arrays (ECCA channel) is demonstrated.
Abstract Protective immunity to mycobacterial infections requires activation of the antibacterial mechanisms infected macrophages. It has previously been reported that ATP treatment mycobacteria-infected macrophages induces apoptosis mediated via P2X7 pathway and this leads death both host cell internalized bacilli. We have recently identified a single nucleotide polymorphism in gene (1513A→C), with 1–2% prevalence homozygous state, which codes for nonfunctional receptor. IFN-γ-primed, from...
White blood cells (WBCs) constitute only about 0.1% of human cells, yet contain rich information the immune status body; thus, separation WBCs from whole is an indispensable and critical sample preparation step in many scientific, clinical, diagnostic applications. In this paper, we developed a continuous high-throughput microfluidic WBC platform utilizing differential inertial focusing particles serpentine microchannels. First, performance proposed method characterized evaluated using...
This paper proposes a novel concept of dielectrophoresis (DEP)-active hydrophoretic focusing micro-particles and murine erythroleukemia (MEL) cells. The DEP-active platform consists crescent shaped grooves interdigitated electrodes that generate lateral pressure gradients. These embedded exert negative DEP force onto the particles by pushing them into narrow space in channel where particle to groove interaction is intensive ordering occurs. Particles passing through microfluidic device are...
Theranostic platforms have emerged as advanced systems integrating diagnostic and therapeutic agents to enable personalized medicine tailored the specific characteristics of each patient's disease. However, conventional theranostic strategies face challenges in achieving high specificity sensitivity for diagnosis therapy. This necessitates development novel improve accuracy efficacy. Peptide-nanoparticle conjugates (PNCs) recently gained attention promising cancer platforms, offering...
This article reviews the integration of multidisciplinary approaches, including protein engineering, computational biology, and nanoarchitectonics, to advance pharmaceutical enzyme biocatalysis.