A5032106074- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Angiogenesis and VEGF in Cancer
- Ocular Diseases and Behçet’s Syndrome
- Retinopathy of Prematurity Studies
- Retinal and Macular Surgery
- Cancer, Hypoxia, and Metabolism
- Complement system in diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Interference and Gene Delivery
- Advanced Glycation End Products research
- Barrier Structure and Function Studies
- Facial Trauma and Fracture Management
- Vascular Malformations Diagnosis and Treatment
- Neurological Complications and Syndromes
- Blood Coagulation and Thrombosis Mechanisms
- Virus-based gene therapy research
- Proteoglycans and glycosaminoglycans research
- Optical Coherence Tomography Applications
- Meningioma and schwannoma management
- Ginkgo biloba and Cashew Applications
- Biomarkers in Disease Mechanisms
University of Central Florida
2014-2025
Orlando College of Osteopathic Medicine
2023-2024
Florida College
2014-2023
Macula and Retina Institute
2018
Winter Haven Hospital
2006-2017
Weatherford College
1998-2014
Penn Presbyterian Medical Center
2002-2005
University of Pennsylvania
2002-2005
Acuity Technologies (United States)
2004
Massachusetts Eye and Ear Infirmary
1993-2000
Error in Figure in: A Randomized Trial Comparing the Efficacy and Safety of Intravitreal Triamcinolone With Observation to Treat Vision Loss Associated Macular Edema Secondary Central Retinal Vein Occlusion: Standard Care vs Corticosteroid for Occlusion (SCORE) Study Report 5
Elevated vascular endothelial growth factor (VEGF) levels are required for ocular and tumor angiogenesis in animal models. Ischemic hypoxia is strongly correlated with increased VEGF expression these systems considered a physiologically relevant stimulus. Because ischemic often followed by reperfusion reactive oxygen intermediate (ROI) generation, we examined the potential role of ROI control gene expression. Human retinal pigment epithelial cells exposed to superoxide or hydrogen peroxide...
Advanced glycation end products (AGEs) are linked with the development of diabetic retinopathy; however, pathogenic mechanisms poorly defined. Vascular endothelial growth factor (VEGF) levels increased in ischemic and nonischemic retina, VEGF is required for retinal iris neovascularization. Moreover, alone can induce much concomitant pathology retinopathy. In this study, we found that AGEs mRNA ganglion, inner nuclear, pigment epithelial (RPE) cell layers rat retina. vitro, secreted protein...
To determine whether the angiogenic peptide vascular endothelial growth factor (VEGF) is sufficient to produce iris neovascularization in a nonhuman primate (Macaca fascicularis).Eight eyes of 4 animals were studied. The 165-amino acid isoform human recombinant VEGF (VEGF165) was injected into vitreous 5 cynomolgus monkey (doses ranging from 0.25-2.5 micrograms per injection). Equal amounts inactivated (2 eyes) or vehicle (1 eye) contralateral control eyes. Eyes assessed by slitlamp...
Insulin-like growth factor I (IGF-I) and vascular endothelial (VEGF) levels are correlated with retinal ischemia-associated intraocular neovascu-larization in humans. Since VEGF is required for iris neovascularization animal models of ischemia, we tested whether IGF-I could act as an indirect angiogenic by increasing gene expression. increased pigment epithelial (RPE) cell mRNA a concentration-dependent manner EC50 7 nmol/l (53.6 ng/ml). RPE bovine smooth muscle cells exposed to 50 nmol/1...
In Brief Purpose To determine the safety and efficacy of small interfering RNA (siRNA) directed against vascular endothelial growth factor (VEGF) in a nonhuman primate model laser-induced choroidal neovascularization (CNV). Methods Each animal received laser rupture Bruch's membrane to induce CNV both eyes. was then randomized receive 0.05 mL either vehicle alone or VEGF siRNA at 70 μg, 150 350 μg eyes by intravitreal injection. Eyes were monitored weekly ophthalmic examination, color...
Neovascularization characterizes diabetic retinopathy and choroidal neovascularization associated with age-related macular degeneration, the most common causes of severe visual loss in developed world. Gene transfer to eye using adeno-associated viral (AAV) vectors is a promising new treatment for inherited acquired ocular diseases. We used an AAV vector rapid onset high levels gene expression retina deliver three anti-angiogenic factors (pigment epithelium-derived factor, tissue inhibitor...
AbstractPurpose. In tissues outside the brain, vascular endothelial growth factor-A (VEGF) causes hyper-permeability by opening of inter-endothelial junctions and induction fenestrations vesiculo-vacuolar organelles (VVOs). preliminary studies, we observed that in blood-retinal barrier endothelium, other cellular mechanisms may underlie increased permeability caused VEGF. This was further investigated material an vivo experimental model VEGF-induced retinopathy. Methods. Two monkeys received...
The alternative pathway of the complement system is implicated in etiology age-related macular degeneration (AMD). Complement depletion with pegcetacoplan and avacincaptad pegol are FDA-approved treatments for geographic atrophy AMD that, while effective, have clinically observed risks choroidal neovascular (CNV) conversion, optic neuritis, retinal vasculitis, leaving room other equally efficacious but safer therapeutics, including Poly Sialic acid (PSA) nanoparticle (PolySia-NP)-actuated...
The control, over time and space, of the levels therapeutic proteins is crucial for successful retinal gene therapy. We tested ability adeno-associated viral vectors (AAV) delivered intraocularly to release a secreted protein (erythropoietin (Epo) used as marker) in eye, either constitutively or pharmacologically regulated manner using dimerizer-inducible transcriptional regulatory system. Following delivery expressing vector intravitreal subretinal space nude rats, Epo was detected both...
<h3>Importance</h3> Macular edema (ME) is the leading cause of decreased visual acuity (VA) associated with retinal vein occlusion (RVO). Identifying factors better outcomes in RVO eyes treated anti–vascular endothelial growth factor (VEGF) therapy may provide information useful counseling patients. <h3>Objective</h3> To investigate baseline characteristics 6-month VA and central subfield thickness (CST) participants Study Comparative Treatments for Retinal Vein Occlusion 2 (SCORE2)....
Age-related macular degeneration (AMD) is a chronic, progressive retinal disease characterized by an inflammatory response mediated activated macrophages and microglia infiltrating the inner layer of retina. In this study, we demonstrate that inhibition through Siglec binding in AMD eye can generate therapeutically useful effects. We show Siglecs-7, -9 -11 are upregulated associated M0 M1 macrophages, these be selectively targeted using polysialic acid (PolySia)-nanoparticles (NPs) to...