A5052707256- Pituitary Gland Disorders and Treatments
- Ovarian function and disorders
- Growth Hormone and Insulin-like Growth Factors
- Hormonal and reproductive studies
- Electrochemical sensors and biosensors
- Adrenal Hormones and Disorders
- Thyroid Disorders and Treatments
- Lipoproteins and Cardiovascular Health
- Hypothalamic control of reproductive hormones
- Neonatal Health and Biochemistry
- Adrenal and Paraganglionic Tumors
- Glycosylation and Glycoproteins Research
- Hormonal Regulation and Hypertension
- Amoebic Infections and Treatments
- High Altitude and Hypoxia
- Monoclonal and Polyclonal Antibodies Research
- Stress Responses and Cortisol
- Bacterial Infections and Vaccines
- Respiratory Support and Mechanisms
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Pharmacological Effects and Assays
- Sexual Differentiation and Disorders
- Malaria Research and Control
- Sperm and Testicular Function
- Computational Drug Discovery Methods
University of Notre Dame
2023-2024
University of Wisconsin–Madison
2020-2022
Para La Tierra
2020-2022
Philadelphia University
2019
Salford Royal Hospital
1994-2016
University of California, San Francisco
1995-2016
Lexington City Schools
2015
Institute of Molecular Biology and Biophysics
2014
University of Salford
1994-2009
University of Manchester
1994-2000
The relation between end-tidal carbon dioxide tension (PETCO2) measured by infrared analysis and arterial (PaCO2) during exercise was systematically examined in five healthy adults at two power outputs (25 50% VO2max) three frequencies of breathing (15, 30, 45 breaths/min). PETCO2-PaCO2 varied -2.5 +9.1 Torr, inversely related to the frequency (r = 0.475), directly tidal volume (VT; r 0.791) CO2 output 0.627). An equation obtained multiple regression analysis, predict PaCO2 from PETCO2: 5.5...
To determine the frequency of polycystic ovaries (PCO) on ultrasound and incidence clearcut endocrine disorders leading to virilization in patients complaining hirsutism or androgenic alopecia. The major purpose was a coherent policy for routine biochemical assessment such women.A prospective study women attending joint skin/endocrine clinic these problems.Three hundred fifty consecutive with and/or alopecia were assessed.Baseline screens conducted two occasions included measurement serum...
Background Less invasive methods are replacing traditional means of measuring coronary flow reserve (CFR). Transesophageal echocardiography (TEE) is becoming a useful tool for evaluation artery disease and has recently been used to measure CFR. This always done using dipyridamole, but adenosine greater vasodilator potency more favorable kinetics than dipyridamole. study was evaluate the hypothesis that safe, rapid, accurate in blood by TEE Doppler. Methods Results Forty-nine patients who had...
A patient with Graves' disease and progressive infiltrative exophthalmos of the right eye failed to respond orbital decompression. The process improved following total surgical hypophysectomy. Interim evaluations suggested that hypophysectomy was complete. Two one-half years after developed severe left eye. Additional study supported conclusion there no demonstrable functioning pituitary tissue. implications recurrent ablation are discussed.
ABSTRACT Piperaquine (PPQ) is widely used in combination with dihydroartemisinin as a first-line treatment against malaria. Multiple genetic drivers of PPQ resistance have been reported, including mutations the Plasmodium falciparum chloroquine transporter ( pfcrt ) and increased copies plasmepsin II/III pm2/3 ). We generated cross between Cambodia-derived multidrug-resistant KEL1/PLA1 lineage isolate (KH004) drug-susceptible Malawian parasite (Mal31). Mal31 harbors wild-type (3D7-like)...
All immunoassays for female serum testosterone give falsely high results in some samples. The effect is variable and cannot be predicted any given sample. Inaccurate calibration or interference by cross-reacting substances almost certainly the cause of problem, but many immunoassays, exact nature interferent not known. Some can removed employing an extraction step prior to immunoassay. advent fast simple sensitive liquid chromatography tandem mass spectrometry methods offers exciting...
There are known difficulties in measuring testosterone women using 'direct' (non-extraction) immunoassays. The positive bias encountered many of these assays has been attributed to an unidentified interfering substance. We have evaluated the potential for dehydroepiandrosterone sulphate (DHEA-S) cross-react Abbott Architect assay.Samples (n = 81) from female patients who had DHEA-S, direct and extracted measured were examined retrospectively. Possible cross-reactivity was by spiking both...
Background: Direct (non-extracted) testosterone immunoassays may give spuriously high results in women. The presumed interferents be removed if is extracted into an organic phase before being measured. We aimed to clarify possible causes and effects of interference measurement Methods: Women who had a blood sample referred Hope Hospital Clinical Biochemistry laboratory for serum concentration over six-month period were studied. treatment data recorded. A difference (direct-minus-extracted...
A method is described for the identification of apolipoprotein E phenotypes. Lipoproteins were precipitated by magnesium chloride/phosphotungstate reagent and isoelectric focusing performed on flat-bed agarose. Sensitivity was enhanced blotting with nitrocellulose prior to staining. The system requires no ultracentrifugation step allows up 20 samples be processed in each run. Agarose has some advantages over more commonly used polyacrylamide gels focusing.
This report describes investigations into the use of sodium salicylate as a cortisol-binding-globulin blocking agent and subsequent development radioimmunoassay for cortisol on unextracted plasma. Cortisol antiserum was raised against 3–0-(carboxymethyl) oxime-bovine serum albumin conjugate. A 125 I-labelled cortisol-tyrosine methyl ester conjugate also prepared in assay. The developed involved no pre-treatment or extraction samples before analysis extremely simple to perform. Comparison...
Determination of urinary free catecholamine and total (i. e., plus conjugated) metanephrine excretion is considered the most clinically sensitive biochemical test for pheochromocytoma. In this study, we evaluated new immunoassay methods measurement these analytes potential drug-based interference.Urine samples collected from patients on a variety medications were grouped by specific drug type. The significance any difference in or concentrations different groups was assessed one-way ANOVA. A...
A woman with hirsutism but otherwise symptom-free was found to have a raised serum prolactin and pituitary microadenoma. The hyperprolactinaemia persisted despite bromocriptine therapy subsequent surgery, which yielded non-functioning adenoma. After further 15 years persistent no symptoms, macroprolactinaemia diagnosed. Such cases might account for part of the failure rate microsurgery prolactinoma. Testing is advisable in hyperprolactinaemia, especially if her ovulatory cycle normal. Two...
Piperaquine (PPQ) is widely used in combination with dihydroartemisinin (DHA) as a first-line treatment against malaria parasites. Multiple genetic drivers of PPQ resistance have been reported, including mutations the
Polycarbonate (PC) membranes have been treated with dimethylamine (DMA), n-pentylamine (PA) or n-heptylamine (HA) in a glow-discharge apparatus. Amino group concentrations on plasma-polymerized PC were then assayed by binding radiolabelled [1-14C]acetic anhydride to the membrane, followed scintillation counting. For different degrees of (125I) glucose oxidase rennet observed and this was found be directly related surface amino-group for appropriate membrane.
Abstract Bertiella sp., a cestode known to infect variety of hosts, including nonhuman primates and humans, was identified in Paraguay as early 1895, but no systematic analysis wild primates’ gastrointestinal parasites has ever been carried out Paraguay. Increased urbanization southwest pushed the Paraguayan howler monkey ( Alouatta caraya) into anthropogenic habitats, particularly city Pilar (Ñeembucú department), giving rise greater potential for zoonotic transmission between humans. From...
Many clinical chemistry laboratories offer thyroid-stimulating hormone (TSH) alone as a first-line test of thyroid function, and only reflex free thyroxine (fT4) if the TSH result is abnormal (i. e., outside laboratory reference range). In secondary hypothyroidism, low fT4 may be accompanied by or normal level. A testing strategy that measures baseline risks missing cases hypothyroidism in which level normal.The current authors examined 26,106 consecutive function (TFT) results our initial...
Abstract Kinetic measurement of an alkaline phosphatase enzyme label was performed with amperometric detection using conversion substrate 4‐aminophenyl phosphate to the electroactive phenolic, measured at +125 +200 mV tvs. Ag/AgCl. Calibration plots for thyroxine‐binding globulin (TBG) and cortisol were obtained by excess reagent limited immunoassays, respectively, which comparable those colorimetric detection. These provided a range 31–1000 μg/L TBG 100–2000 nM cortisol. An antibody‐bound...
Prolactin circulates predominantly as a 23-kDa monomer, and high-molecular-weight form largely consisting of complex prolactin an anti-prolactin IgG autoantibody, called macroprolactin. This cross-reacts with conventional laboratory assays for prolactin. We here describe how quantitative adjustment this may assist patient management.In consecutive series 218 patients elevated to 400 mu/L or more in men (normal range ≤ 180) (n=79, 36.2% sample) 1 000 women 500) (n=139, 63.8%) macroprolactin...