A5077655975- Algal biology and biofuel production
- Microbial Metabolic Engineering and Bioproduction
- Biodiesel Production and Applications
- Biofuel production and bioconversion
- Marine and coastal ecosystems
- Marine Bivalve and Aquaculture Studies
- Food Industry and Aquatic Biology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Nutrition, Genetics, and Disease
- Enzyme Catalysis and Immobilization
- Microbial metabolism and enzyme function
- Photosynthetic Processes and Mechanisms
- Aquaculture Nutrition and Growth
- Metabolomics and Mass Spectrometry Studies
- Marine and coastal plant biology
- Water Quality Monitoring and Analysis
- Mass Spectrometry Techniques and Applications
- Seaweed-derived Bioactive Compounds
- Spectroscopy and Chemometric Analyses
- Analytical Chemistry and Chromatography
- Aquaculture disease management and microbiota
- Microbial Community Ecology and Physiology
- Protein Hydrolysis and Bioactive Peptides
- Gut microbiota and health
- Anaerobic Digestion and Biogas Production
National Renewable Energy Laboratory
2016-2025
Office of Scientific and Technical Information
2014
John Innes Centre
2011
Pennsylvania State University
2011
Bangor University
2010
Following scrutiny of present biofuels, algae are seriously considered as feedstocks for next-generation biofuels production. Their high productivity and the associated lipid yields make them attractive options. In this review, we analyse a number aspects large-scale overall algal biomass production from biochemical energetic standpoint. We illustrate that maximum conversion efficiency total solar energy into primary photosynthetic organic products falls in region 10%. Biomass composition...
ABSTRACT The accumulation of bioenergy carriers was assessed in two starchless mutants Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7 - 10 [isoamylase] mutants), a control strain (CC124), complemented strains the mutant. results indicate that genetic blockage starch synthesis increases lipids on cellular basis during nitrogen deprivation relative to CC124 as determined by conversion fatty acid methyl esters. However, this increased level lipid is energetically...
Algal biomass compositional analysis data form the basis of a large number techno-economic process models that are used to investigate and compare different processes in algal biofuels production. However, analytical methods generate these far from standardized. This work investigated applicability common for rapid chemical samples with respect accuracy precision. study measured lipids, protein, carbohydrates, ash, moisture single sample at 3 institutions by 8 independent researchers over 12...
Biomass and bioproduct composition critical improvements are a priority for the nascent algae-based bioeconomy.
The development of an integrated biorefinery process capable producing multiple products is crucial for commercialization microalgal biofuel production. Dilute acid pretreatment has been demonstrated as efficient approach to utilize algal biomass more fully, by hydrolyzing carbohydrates into fermentable sugars, while making the lipids extractable, and a protein fraction available other products. Previously, we have shown that sugar-rich liquor could be separated from solid residue...
One of the major challenges associated with algal biofuels production in a biorefinery-type setting is improving biomass utilization its entirety, increasing process energetic yields and providing economically viable scalable co-product concepts.
In the context of algal biofuels, lipids, or better aliphatic chains fatty acids, are perhaps most important constituents biomass. Accurate quantification lipids and their respective fuel yield is crucial for comparison strains growth conditions process monitoring. As an alternative to traditional solvent-based lipid extraction procedures, we have developed a robust whole-biomass in situ transesterification procedure (as acid methyl esters, FAMEs) that (a) can be carried out on small scale...
Structural carbohydrates can constitute a large fraction of the dry weight algal biomass and thus accurate identification quantification is important for summative mass closure. Two limitations to characterization microalgal are lack robust analytical procedure hydrolyze polymeric their respective monomers subsequent those monosaccharides. We address second limitation, chromatographic separation monosaccharides, here by identifying optimum conditions resolution synthetic mixture 13...
A large number of algal biofuels projects rely on a lipid screening technique for selecting particular strain with which to work. We have developed multivariate calibration model predicting the levels spiked neutral and polar lipids in microalgae, based infrared (both near-infrared (NIR) Fourier transform (FTIR)) spectroscopy. The advantage an spectroscopic over traditional chemical methods is direct, fast, non-destructive nature method. This provides fast high-throughput method determining...
Methane-utilizing bacteria (methanotrophs) are capable of growth on methane and attractive systems for bio-catalysis. However, the application natural methanotrophic strains to large-scale production value-added chemicals/biofuels requires a number physiological genetic alterations. An accurate metabolic model coupled with flux balance analysis can provide solid interpretative framework experimental data analyses integration. A stoichiometric Methylomicrobium buryatense strain 5G(B1) was...
Methane is a feedstock of interest for the future, both from natural gas and renewable biogas sources. Methanotrophic bacteria have potential to enable commercial methane bioconversion value-added products such as fuels chemicals. A strain applications Methylomicrobium buryatense 5GB1, due its robust growth characteristics. However, take advantage this methanotroph, it important generate comprehensive bioreactor-based datasets different conditions compare bioprocess parameters. Datasets...
Green pathways for nonisocyanate polyurethane (NIPU) production have attracted increasing levels of interest. The reaction between 5-membered cyclic carbonate and polyamines is one the most promising to produce NIPU polymers. Though promising, major technical hurdles such as slow polymerization rate poor performance hinder commercialization NIPU. In this paper, we screened several commercially available triglyceride oil feedstocks products, focusing on kinetics product industrial...
Abstract Background Gut microorganisms aid in the digestion of food by providing exogenous metabolic pathways to break down organic compounds. An integration longitudinal microbial and chemical data is necessary illuminate how gut supplement energetic nutritional requirements animals. Although mammalian systems are well-studied this capacity, role microbes breakdown utilization recalcitrant marine macroalgae herbivorous fish relatively understudied an emerging priority for bioproduct...