A5058167476- Alzheimer's disease research and treatments
- Supramolecular Self-Assembly in Materials
- Lipid Membrane Structure and Behavior
- Acoustic Wave Resonator Technologies
- RNA Research and Splicing
- Biosensors and Analytical Detection
- Probiotics and Fermented Foods
- Advanced Biosensing Techniques and Applications
- Magnetic Properties and Applications
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Advanced biosensing and bioanalysis techniques
- Prion Diseases and Protein Misfolding
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Characterization and Applications of Magnetic Nanoparticles
- Phytoplasmas and Hemiptera pathogens
- RNA and protein synthesis mechanisms
- 3D Printing in Biomedical Research
- Extracellular vesicles in disease
- Proteins in Food Systems
Lund University
2023-2025
University of Guelph
2014
Shear forces affect self-assembly processes ranging from crystallization to fiber formation. Here, the effect of mild agitation on amyloid fibril formation was explored for four peptides and investigated in detail A <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline" overflow="scroll"> <mml:mrow> <mml:mi mathvariant="bold">β</mml:mi> </mml:mrow> </mml:math> 42, which is associated with Alzheimer’s disease. To gain mechanistic insights into agitation, nonseeded seeded...
Formation of amyloid-β (Aβ) fibrils is a central pathogenic feature Alzheimer's disease. Cell-secreted extracellular vesicles (EVs) have been suggested as disease modulators, although their exact roles and relations to Aβ pathology remain unclear. We combined kinetics assays biophysical analyses explore how small (<220 nm) EVs from neuronal non-neuronal human cell lines affected the aggregation disease-associated variant Aβ(1-42) into amyloid fibrils. Using thioflavin-T monitored seeding...
The nucleation of amyloid fibrils from monomeric protein, catalyzed by the surface existing fibrils, is an important driver many disorders such as Alzheimer's and Parkinson's diseases. structural basis this secondary process, however, poorly understood. Here, we ask whether sites are found predominantly at rare growth defects: defects in fibril core structure generated during their original assembly. We first demonstrate using specific inhibitor nucleation, Brichos, that on...
Amyloid β peptide (Aβ) is the crucial protein component of extracellular plaques in Alzheimer's disease. The also contain gangliosides lipids, which are abundant membranes neuronal cells and cell-derived vesicles exosomes. When present at concentrations above its critical micelle concentration (cmc), can occur as mixed micelles. Here, we study coassembly ganglioside GM1 Aβ peptides Aβ40 42 by means microfluidic diffusional sizing, confocal microscopy, cryogenic transmission electron...