A5011961568- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Marine Sponges and Natural Products
- Bioactive Compounds and Antitumor Agents
- Synthetic Organic Chemistry Methods
- Cytokine Signaling Pathways and Interactions
- Natural product bioactivities and synthesis
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Inflammatory mediators and NSAID effects
- Microbial Metabolism and Applications
- Cancer Treatment and Pharmacology
- Phytochemical compounds biological activities
- Phytochemistry and Bioactivity Studies
- Cancer therapeutics and mechanisms
- Synthesis and biological activity
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Response and Inflammation
- Genomics, phytochemicals, and oxidative stress
- TGF-β signaling in diseases
- Cancer-related gene regulation
- Steroid Chemistry and Biochemistry
- NF-κB Signaling Pathways
- Atherosclerosis and Cardiovascular Diseases
- Phytochemistry and Bioactive Compounds
University of Kaiserslautern
2014-2024
Rheinland-Pfälzische Technische Universität Kaiserslautern-Landau
2023-2024
University of Koblenz and Landau
2023
Johannes Gutenberg University Mainz
2008-2015
Institut für Biotechnologie und Wirkstoff-Forschung
2000-2012
Heidelberg University
2012
University Hospital Heidelberg
2012
University Medical Center of the Johannes Gutenberg University Mainz
2012
University Medical Centre Mannheim
2012
Schrodinger (United States)
2000-2007
Apples (Malus spp., Rosaceae) and products thereof contain high amounts of polyphenols which show diverse biological activities may contribute to beneficial health effects, like protecting the intestine against inflammation initiated by chronic inflammatory bowel diseases (IBD). IBD are characterized an excessive release several proinflammatory cytokines chemokines different cell types results consequently in increased response. In present study we investigated preventive effectiveness...
The spike protein is the major on surface of coronaviruses. It therefore prominent target neutralizing antibodies and consequently antigen all currently admitted vaccines against SARS-CoV-2. Since it a 1,273-amino acids glycoprotein with 22 N-linked glycans, production functional, full-length was limited to higher eukaryotes. Here we report SARS-CoV-2 - lacking C-terminal membrane anchor as secreted in prefusion-stabilized conformation unicellular green alga Chlamydomonas reinhardtii. We...
A search for inhibitors of the IL‐6‐mediated signal transduction in HepG2 cells using secreted alkaline phosphatase (SEAP) as reporter gene resulted isolation galiellalactone (1) from fermentations ascomycete strain A111‐95. Galiellalactone inhibits IL‐6‐induced SEAP expression with IC 50 values 250–500 nM by blocking binding activated Stat3 dimers to their DNA sites without inhibiting tyrosine and serine phosphorylation transcription factor. Due its selective activity, may serve a lead...
(S)-Curvularin and its 13-, 14-, 16-membered lactone homologues were synthesized through a uniform strategy in which Kochi oxidative decarboxylation ring-closing metathesis reactions constitute the key processes. In evaluation of anti-inflammatory effects compounds assays using cells stably transfected with human iNOS promoter-luciferase reporter gene construct, 14- showed slightly higher inhibitory effect towards promoter activity than curvularin itself. However, larger ring also exhibited...
Signal transducer and activator of transcription (STAT)-3 inhibitors play an important role in regulating immune responses. Galiellalactone (GL) is a fungal secondary metabolite known to interfere with the binding phosphorylated signal (pSTAT)-3 as well pSTAT-6 dimers their target DNA vitro. Intra nasal delivery 50 μg GL into lung naive Balb/c mice induced FoxP3 expression locally IL-10 production IL-12p40 RNA airways vivo. In murine model allergic asthma, significantly suppressed cardinal...
In previous studies, we identified the fungal macrocyclic lactone (<i>S</i>)-curvularin (SC) as an anti-inflammatory agent using a screening system detecting inhibitors of Janus kinase/signal transducer and activator transcription pathway. The objective present study was to investigate whether SC is able decrease proinflammatory gene expression in vivo model chronic inflammatory disease. Therefore, effects dexamethasone were compared collagen-induced arthritis (CIA) mice. Total genomic...
In a screening program of natural compounds from fungi, the known cyclopentanoid sesquiterpene (-)-cyclonerodiol was identified as specific inhibitor IL-4 induced STAT6 signaling pathway (IC50 = 9.7 μM) which is required for differentiation naive CD4 T cells to helper type 2 (Th2) lymphocytes. As many allergic conditions, including asthma and atopic diseases, are driven by an excessive Th2 response, promising target development new therapeutics. The compound synthesized in six steps...
The induction of human inducible nitric-oxide synthase (iNOS) expression depends (among other factors) on activation the signal transducer and activator transcription 1 (STAT1) pathway. Therefore, STAT1 pathway may be an appropriate target for development inhibitors iNOS expression. HeLa S3 cells transiently transfected with a γ-activated site (GAS)/interferon-stimulated response element-driven reporter gene construct were used as primary screening system. Using this system, three novel...
Survivin, a member of the IAP (inhibitor apoptosis) gene family, is overexpressed in virtually all human cancers and functionally involved inhibition apoptosis, regulation cell proliferation, metastasis resistance to therapy. Because its upregulation malignancy, survivin has currently attracting considerable interest as new target for anticancer In screening approximately 200 strains imperfect fungi production inhibitors promoter activity, drimane sesquiterpene lactone, SF002-96-1, was...
Herein, the first total synthesis of natural 13-hydroxy-14-deoxyoxacyclododecindione along with revision proposed stereoconfiguration is reported. This product, initially discovered in 2018, belongs to oxacyclododecindione family, renowned for their remarkable anti-inflammatory and antifibrotic activities. The synthetic route involves an esterification/Friedel-Crafts-acylation approach uses various triol fragments. It allows preparation different stereoisomers, including (revised) two...
Abstract Various synthetic approaches to the oxacyclododecindione‐type macrolactones, known for their potent anti‐inflammatory activity, are presented. These include an attempted carbonylative ring closure, a hydroacylation route, and approach by ring‐closing metathesis double bond isomerization, as well strategy including metathesis/unsaturation. The last route allowed preparation of bioactive analogue recently described 14‐deoxyoxacyclododecindione.