A5058148743- Bacillus and Francisella bacterial research
- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
- Insect Resistance and Genetics
- SARS-CoV-2 and COVID-19 Research
- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Protein purification and stability
- Plant Pathogenic Bacteria Studies
- Yersinia bacterium, plague, ectoparasites research
- Metal-Catalyzed Oxygenation Mechanisms
- Studies on Chitinases and Chitosanases
- Protein Hydrolysis and Bioactive Peptides
- Legume Nitrogen Fixing Symbiosis
- Viral Infections and Outbreaks Research
- Bat Biology and Ecology Studies
- SARS-CoV-2 detection and testing
- Microbial bioremediation and biosurfactants
- Glycosylation and Glycoproteins Research
- Diatoms and Algae Research
- Transgenic Plants and Applications
- Plant Parasitism and Resistance
- Bacterial Genetics and Biotechnology
- Inhalation and Respiratory Drug Delivery
- vaccines and immunoinformatics approaches
University of California, Davis
2022-2024
Cornell University
2024
Israel Institute for Biological Research
1997-2024
Scion
2018
Museum of New Zealand Te Papa Tongarewa
2018
Center for Biologics Evaluation and Research
2006-2008
United States Food and Drug Administration
2007
Agricultural Research Organization
1988-1997
Weizmann Institute of Science
1990
Respiratory anthrax, in the absence of early antibiotic treatment, is a fatal disease. This study aimed to test efficiency therapy curing infected animals and those sick with anthrax. Postexposure prophylaxis (24 h postinfection [p.i.]) guinea pigs intranasally Bacillus anthracis Vollum spores doxycycline, ofloxacin, imipenem, gentamicin conferred protection. However, upon termination died from respiratory Combined treatment antibiotics active vaccination protective antigen-based vaccine...
The COVID-19 pandemic demonstrated a pressing need for rapid, adaptive, and scalable manufacturing of vaccines reagents. With the transition into an endemic disease rising threats other emerging pandemics, production these biologicals requires stable sustainable supply chain accessible distribution methods. In this study, we demonstrate strength engineered filamentous fungal platform, Thermothelomyces heterothallica C1, high volumetric productivity full-length spike glycoprotein. Spike...
Human alpha1-proteinase inhibitor (alpha1-PI), also known as antitrypsin, is the most abundant serine protease (serpin) in plasma. Its deficiency associated with development of progressive, ultimately fatal emphysema. Currently United States, alpha1-PI available for replacement therapy an FDA licensed plasma-derived (pd) product. However, plasma source itself limited; moreover, even efficient viral inactivation steps used manufacture products, risk contamination from emerging viruses may...
Coronavirus disease 2019 (COVID-19) has caused the ongoing COVID-19 pandemic and there is a growing demand for safe effective vaccines. The thermophilic Thermothelomyces heterothallica filamentous fungal host, C1-cell, can be utilized as an expression platform rapid production of large quantities antigens developing aim this study was to evaluate local tolerance systemic toxicity C1-cell expressed receptor-binding domain (C1-RBD) vaccine, following repeated weekly intramuscular injections...
Human alpha one proteinase inhibitor (alpha1-PI) was cloned and expressed in Aspergillus niger, filamentious fungus that can grow defined media perform glycosylation. Submerged culture conditions were established using starch as carbon source, 30% dissolved oxygen concentration, pH 7.0 28 degrees C. Eight milligrams per liter of active alpha1-PI secreted to the growth about 40 h. Controlling protein proteolysis found be an important factor production. The effects various sources, temperature...
SARS-CoV-2 is evolving with increased transmission, host range, pathogenicity, and virulence. The original mutant viruses escape innate (Interferon) immunity adaptive (Antibody) immunity, emphasizing unmet needs for high-yield, commercial-scale manufacturing to produce inexpensive vaccines/boosters global/equitable distribution. We developed DYAI-100A85, a spike receptor binding domain (RBD) subunit antigen vaccine expressed in genetically modified thermophilic filamentous fungus,...
We present the DNA sequence of some 3590 base pairs from virulence plasmid crown-gall-forming bacterium Erwinia herbicola pv. gypsophilae strain PD713. This region includes entire transcription unit an operon encompassing IAA synthesis genes. These genes, designated iaaM and iaaH , reside on a 78-MDa native plasmid. The analysis indicates that contains two open reading frames 562 ( ) 460 amino acids ), corresponding to proteins with molecular weight 62,473 49,300 daltons, respectively. When...
A novel COVID-19 vaccine (BriLife®) has been developed by the Israel Institute for Biological Research (IIBR) to prevent spread of SARS-CoV-2 virus throughout population in Israel. One components formulation is tris(hydroxymethyl)aminomethane (tromethamine, TRIS), a buffering agent. TRIS commonly used excipient various approved parenteral medicinal products, including mRNA vaccines produced Pfizer/BioNtech and Moderna. hydrophilic basic compound that does not contain any...
Biomanufacturing enables novel sources of compounds with constant demand, such as food coloring and preservatives, well new peak diagnostics vaccines. The COVID-19 pandemic has highlighted the need for alternative research materials, thrusting on diversification biomanufacturing platforms. Here, we show initial results exploring walnut somatic embryogenic system expressing recombinant receptor binding domain (RBD) ectodomain spike protein (Spike) from SARS-CoV-2 virus. Stably transformed...
The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has resulted in more than seven million deaths worldwide. To reduce viral spread, the Israel Institute for Biological Research (IIBR) developed and produced a new rVSV-SARS-CoV-2-S vaccine candidate (BriLife®) based on platform of genetically engineered vesicular stomatitis virus (VSV) vector that expresses spike protein SARS-CoV-2 instead VSV-G surface. Quantifying titer to evaluate potency...