Plasmodium falciparum, the malaria-causing parasite, is a leading cause of infection-induced deaths worldwide. The preferred treatment approach artemisinin-based combination therapy, which couples fast-acting artemisinin derivatives with longer-acting drugs, such as lumefantrine, mefloquine, and amodiaquine. However, urgency for new treatments has risen due to parasite's growing resistance existing therapies. In this study, common characteristic P. falciparum proteome—stretches poly-lysine...

Enhancer RNAs (eRNAs) are transcribed by RNA polymerase II during enhancer activation but typically rapidly degraded in the nucleus. During states of reduced surveillance, however, eRNAs and other similar "noncoding" RNAs, including for example upstream antisense stabilized, some exported to cytoplasm can even be found on polysomes. Here, we report unexpectedly that ∼12% human intergenic contain long open reading frames (>300 nts), many which actively translated, as determined ribosome...

In this paper, chromatographic analysis of active substance olopatadine hydrochloride, which is used in eye drops as antihistaminic agent, and its impurity E isomer by hydrophilic interaction liquid chromatography (HILIC) application design experiments (DoE) methodology are presented. addition, benzalkonium chloride very often a preservative drops. Therefore, the evaluation behavior HILIC was carried out well. order to estimate set optimal conditions, DoE applied. After selection important...

Oligosaccharides represent complex class of analytes for mass spectrometric analysis due to the high variety structural isomers concerning glycosidic linkages and possible branching. A systematic study negative ion mode matrix‐assisted laser desorption/ionization (MALDI) spectrometry various neutral oligosaccharides under selection an appropriate matrix, like 2,5‐dihydroxyacetophenone (2,5‐DHAP) is reported here, without commonly used anion dopant strategies. Nevertheless, we were able...

This paper presents the chemometrically assisted optimization and validation of RP-HPLC method intended for quantitative analysis itraconazole its impurities in pharmaceutical dosage forms. To reach desired chromatographic resolution with a limited number experiments minimum amount time, Box- -Behnken design was used to simultaneously optimize some important parameters, such as acetonitrile content mobile phase, pH aqueous phase column temperature. Separation between impurity F identified...

In this paper, the retention prediction models for mixture of β‐lactam antibiotics analyzed by hydrophilic interaction chromatography ( HILIC ) are presented. The aim study was to investigate behavior some organic acids and amphoteric compounds including cephalosporins (cefotaxime, cefalexin, cefaclor, cefuroxime, cefuroxime axetil) penicillins (ampicillin amoxicillin). Retention substances with acidic functional group in is considered be interesting since majority publications literature...

Ribosomes are macromolecular RNA-protein complexes that constitute the central machinery responsible for protein synthesis and quality control in cell. also serve as a hub multiple non-ribosomal proteins RNAs synthesis. However, purification of ribosomes associated factors functional structural studies requires large amount starting biological material tedious workflow. Current methods challenging they combine ultracentrifugation, use sucrose cushions or gradients, expensive equipment, hours...

Human milk oligosaccharides (HMO) represent the bioactive components of human milk, influencing infant's gastrointestinal microflora and immune system. Structurally, they a highly complex class analyte, where main core oligosaccharide structures are built from galactose N-acetylglucosamine, linked by 1-3 or 1-4 glycosidic linkages potentially modified with fucose sialic acid residues. The can be linear branched. Additional structural complexity in samples induced endogenous exoglycosidase...

Hydrophilic interaction liquid chromatography (HILIC) has emerged as an alternative separation technique for small polar compounds. Design of Experiments (DoE) methodology in the analysis retention behavior and method development have been shown to be successful. This article addresses optimization chromatographic a model mixture consisting four sympathomimetic drugs (ephedrine, pseudoephedrine, phenylephrine, synephrine), on bare silica column by HILIC. The acetonitrile content, pH aqueous...

Rationale Investigation of non‐covalent complexes proteins using Affinity Mass Spectrometry (AMS) represents a major challenge in modern biomedical research. However, many experimental obstacles can make AMS data analysis complex. Additionally, sample purity and size the protein may still pose significant challenges. Methods Matrix‐assisted laser desorption/ionization‐time‐of‐flight (MALDI‐TOF) mass spectrometry (MS) was used for initial mapping samples. nanoESI (electrospray ionization)...

This paper presents the robust optimization of hydrophilic interaction liquid chromatographic method for analysis bisoprolol and its impurities A C. Chemometric strategy is applied detailed understanding system's behavior establishing mathematical relationship between investigated factors (acetonitrile content in mobile phase, pH water phase buffer concentration phase) responses. Grid point search methodology then performed with aim to identify satisfactory separation quality all analyzed...

The retention behavior of substances in hydrophilic interaction liquid chromatography (HILIC) is difficult to predict. Mixture investigated this paper consists acetylsalicylic acid, amlodipine, impurity A and atenolol, a very often used combination treatment some cardiovascular diseases.

Beeswax is one of the most important products for well-being bee colonies. The wax glands young worker bees produce beeswax, which serves as a building material honeycomb construction. Beekeepers using hives with mobile frames mainly utilize local beeswax to make foundations. Any paraffin addition represents adulteration, resulting in high degree contamination. During preparation re-used losses during process may instigate producers add cheaper, wax-like substances like and tallow. This...

Qualitative analysis of glycomic tandem mass spectrometry (MS/MS) data based on m/z values product ions alone is widely used, and often sufficient for single analytes. However, most complex mixtures contain multiple isobaric oligosaccharides, in which case this approach limited. Here we show how ion intensity information can be used order to enhance MS/MS analysis, extract both qualitative semiquantitative from datasets.A matrix-assisted laser desorption/ionization quadrupole time-of-flight...

, the malaria-causing parasite, is a leading cause of infection-induced deaths worldwide. The preferred treatment approach artemisinin-combination therapy, which couples fast-acting artemisinin derivatives with longer-acting drugs like lumefantrine, mefloquine, and amodiaquine. However, urgency for new treatments has risen due to parasite's growing resistance existing therapies. Our study shows that common characteristic

Abstract Metabolic regulation has been recognized as a powerful principle guiding immune responses. Inflammatory macrophages undergo extensive metabolic rewiring marked by the production of substantial amounts itaconate, which recently described an immunoregulatory metabolite. Itaconate and its membrane-permeable derivative dimethyl itaconate (DI) selectively inhibit subset cytokines, including IL-6 IL-12 but not TNF. The major effects on cellular metabolism during macrophage activation have...