A5006187777- Retinal Development and Disorders
- Cancer, Hypoxia, and Metabolism
- interferon and immune responses
- Retinoids in leukemia and cellular processes
- Spaceflight effects on biology
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Cytokine Signaling Pathways and Interactions
- Systemic Lupus Erythematosus Research
- Connexins and lens biology
- Bipolar Disorder and Treatment
- Schizophrenia research and treatment
- Intraocular Surgery and Lenses
- Neurobiology and Insect Physiology Research
- Osteoarthritis Treatment and Mechanisms
- Photochromic and Fluorescence Chemistry
- Physiological and biochemical adaptations
- Knee injuries and reconstruction techniques
- Ophthalmology and Visual Impairment Studies
- Corporate Governance and Management
- Amino Acid Enzymes and Metabolism
- Phagocytosis and Immune Regulation
- High Altitude and Hypoxia
- Erythrocyte Function and Pathophysiology
- Genetic and Kidney Cyst Diseases
Lucerne University of Applied Sciences and Arts
2018-2025
RWTH Aachen University
2024
Asklepios Klinik Langen
2019
University of Zurich
2006-2016
National Institute for Health Research
2010
University Hospital of Zurich
2009
Swiss Integrative Center for Human Health
2009
University Medical Center Hamburg-Eppendorf
2005
Institut für Informationsverarbeitung
2002
Survival and death of photoreceptors in degenerative diseases the retina is controlled by a multitude genes endogenous factors. Some may be involved process itself whereas others part an defense system. We show two models retinal degeneration that photoreceptor strongly induces expression leukemia inhibitory factor (LIF) subset Muller glia cells inner nuclear layer retina. LIF essential to induce extensive intraretinal signaling system which includes characterized upregulation Edn2, STAT3,...
Abstract Exposure to acute and prolonged microgravity triggers numerous physiological adaptations. To date, the underlying molecular mechanisms are not well understood, several pathways have been proposed. Among other candidates, specific ion channels hypothesized be gravity dependent, but it has possible conclusively demonstrate dependency of protein entities. Therefore, we developed a miniaturized two-electrode voltage clamp (TEVC) that allowed electrophysiological experiments on Xenopus...
HIF1A is one of the major transcription factors that regulate tissue response to low oxygen tension. It controls expression a large number genes involved in cell survival, proliferation, angiogenesis, and other cellular processes. present at increased levels early postnatal retina. In this study its potential function during development mouse retina retinal vasculature was analyzed.A line generated with Cre-mediated Hif1a knockdown peripheral Retinal morphology were analyzed sections flat...
The peroxisome proliferator-activated receptor γ coactivator 1 (PGC-1) proteins are key regulators of cellular bioenergetics and accordingly expressed in tissues with a high energetic demand. For example, PGC-1α PGC-1β control organ function brown adipose tissue, heart, brain, liver skeletal muscle. Surprisingly, despite their prominent role the mitochondrial biogenesis oxidative metabolism, expression PGC-1 coactivators retina, an one highest energy demands per tissue weight, completely...
Due to the limited self-repair capacity of articular cartilage, surgical restoration defective cartilage remains a major clinical challenge. The cell-based approach, which is known as autologous chondrocyte transplantation (ACT), has success, presumably because chondrocytes acquire fibroblast-like phenotype in monolayer culture. This unwanted dedifferentiation process typically addressed by using three-dimensional scaffolds, pellet culture, and/or application exogenous factors. Alternative...
PURPOSE: To detect free radicals during phacoemulsification in different use cases and with emulsification of lens tissue. SETTING: Institute for Interfacial Engineering Biotechnology, Fraunhofer Institute, Stuttgart, Germany. DESIGN: Experimental study. METHODS: Three experimental setups were used: 1. Setup without running fluidics to produce air bubbles inside the system. 2. Circulation fluidic setup compare measurements cataractous material. 3. A surgery-comparable system that closely...
Purpose.: Hypoxic preconditioning activates hypoxia-inducible transcription factors (HIFs) in the retina and protects photoreceptors from light-induced retinal degeneration. The authors tested whether photoreceptor-specific activation of HIFs normoxia is sufficient for protection. Methods.: Rod-specific Vhl knockdown mice were generated using Cre-lox system with rod opsin promoter controlling expression CRE recombinase to stabilize HIF normoxic rods. Cell death was induced by light exposure...
Abstract Hypoxic preconditioning stabilizes hypoxia‐inducible factor (HIF) 1α in the retina and protects photoreceptors against light‐induced cell death. HIF‐1α is one of major transcription factors responding to low oxygen tension can differentially regulate a large number target genes. To analyse whether photoreceptor‐specific expression essential protect by hypoxic preconditioning, we knocked down specifically photoreceptor cells, using cyclization recombinase (Cre)–lox system. The...
We introduce a new benchtop microgravity simulator (MGS) that is scalable and easy to use. Its working principle similar of random positioning machines (RPM), commonly used in research laboratories regarded as one the gold standards for simulating microgravity. The improvement MGS concerns mainly algorithms controlling movements samples design that, first time, guarantees equal treatment all culture flasks undergoing simulated Qualification validation tests device were conducted with human...
J. Neurochem. (2010) 113 , 1210–1220. Abstract Retinal degeneration causes the induction of a leukemia inhibitory factor (LIF)‐controlled survival pathway which includes Janus kinase/signal transducer and activator transcription signaling. Lack LIF prevents activation this signaling cascade accelerates disease progression leading to fast loss photoreceptor cells. In study, we show that expression kinase 3 ( Jak 3), but not other members family kinases, is induced in four different models...
Abstract Adequate mechanical stimulation is essential for cellular health and tissue maintenance, including articular cartilage, which lines the articulating bones in joints. Chondrocytes, are sole cells found responsible matrix synthesis, maintenance degradation. It generally believed that chondrocytes require stimuli through daily physical activity adequate cartilage homeostasis. However, to date, molecular mechanisms of force sensing (mechanotransduction) not fully understood. Among other...
To validate an improved wet-lab model for systematic and objective efficiency testing of instruments phacoemulsification.