A5058687214- Extracellular vesicles in disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Circular RNAs in diseases
- Pancreatic function and diabetes
- Bacterial Infections and Vaccines
- Mitochondrial Function and Pathology
- Adenosine and Purinergic Signaling
- Calcium signaling and nucleotide metabolism
- Parkinson's Disease Mechanisms and Treatments
- Long-Term Effects of COVID-19
National Institute on Aging
2022-2024
Johns Hopkins University
2024
Institute on Aging
2022-2024
National Institutes of Health
2024
Florida State University
2021
Alzheimer's disease (AD) is an irreversible neurodegenerative disorder that affects more than 44 million people worldwide. Despite the high burden, there no effective treatment for suffering from AD. Mesenchymal stem cells (MSCs) are multipotent stromal have been widely studied due to their therapeutic potential. However, administration of has found a multitude limitations. Recently, extracellular vesicles (EVs) derived MSCs as candidate, they exhibit similar immunoprotective and...
Besides motor symptoms, many individuals with Parkinson's disease develop cognitive impairment perhaps due to coexisting α-synuclein and Alzheimer's pathologies impaired brain insulin signalling. Discovering biomarkers for in could help clarify the underlying pathogenic processes improve diagnosis prognosis. This study used plasma samples from 273 participants: 103 normal cognition, 121 (81 mild impairment, 40 dementia) 49 age- sex-matched controls. Plasma extracellular vesicles enriched...
Abstract Declining nicotinamide adenine dinucleotide (NAD + ) concentration in the brain during aging contributes to metabolic and cellular dysfunction is implicated pathogenesis of aging‐associated neurological disorders. Experimental therapies aimed at boosting NAD levels normalize several neurodegenerative phenotypes animal models, motivating their clinical translation. Dietary intake precursors, such as riboside (NR), a safe effective avenue for augmenting peripheral tissues humans, yet...
Abstract Isolation of neuron‐derived extracellular vesicles (NDEVs) with L1 Cell Adhesion Molecule (L1CAM)‐specific antibodies has been widely used to identify blood biomarkers CNS disorders. However, full methodological validation requires demonstration L1CAM in individual NDEVs and lower levels or absence EVs from other cells. Here, we multiple single‐EV techniques establish the neuronal origin determine abundance L1CAM‐positive human blood. epitopes ectodomain are shown be co‐expressed on...
Sodium-glucose cotransporter-2 inhibitors (SGLT2is), such as empagliflozin, lower blood glucose in type 2 diabetes mellitus and improve cardiorenal outcomes regardless of presence. Whether SGLT2is exert any effects on the brain’s metabolism has not been studied. We conducted a single-arm clinical trial to investigate once daily administration oral empagliflozin (25 mg) for 14 days systemic brain 21 non-diabetics aged 55 years old or older. Empagliflozin lowered circulating insulin elevated...
To assess whether the rate of change in synaptic proteins isolated from neuronally enriched extracellular vesicles (NEVs) is associated with brain and retinal atrophy people multiple sclerosis (MS).
Mitochondrial dysfunction plays an important role in multiple sclerosis (MS) disease progression. Plasma extracellular vesicles are a potential source of novel biomarkers MS, and some these derived from mitochondria contain functional mitochondrial components.To evaluate the relationship between levels complex IV V activity neuronally enriched (NEVs) brain retinal atrophy as assessed using serial magnetic resonance imaging (MRI) optical coherence tomography (OCT).Our cohort consisted 48...
Abstract The immunoaffinity isolation of neuron-derived extracellular vesicles (NDEVs) from blood by targeting the neuronal protein L1CAM has been widely adopted to provide peripheral biomarkers for brain disorders. However, compelling evidence presence on NDEVs at single-EV level missing. Here, we demonstrate that co-localizes with EV-characteristic and markers in plasma EVs, quantify enrichment achieved after immunoprecipitation, validating NDEV methodology.