Samuel D’Souza
A5045673956- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Genetics, Aging, and Longevity in Model Organisms
- Congenital heart defects research
- Zebrafish Biomedical Research Applications
- Invertebrate Immune Response Mechanisms
- Advanced Fluorescence Microscopy Techniques
Chan Zuckerberg Initiative (United States)
2022-2024
Aging is characterized by a decline in tissue function, but the underlying changes at cellular resolution across organism remain unclear. Here, we present Fly Cell Atlas, single-nucleus transcriptomic map of whole aging
ABSTRACT Elucidating the developmental processes of organisms requires a comprehensive understanding cellular lineages in spatial, temporal, and molecular domains. In this study, we introduce Zebrahub, dynamic atlas zebrafish embryonic development that integrates single-cell sequencing time course data with lineage reconstructions facilitated by light-sheet microscopy. This offers high-resolution in-depth insights into development, achieved through individual embryos across ten stages,...
Abstract Aging is characterized by a decline in tissue function, but the underlying changes at cellular resolution across organism remain unclear. Here, we present Fly Cell Atlas, single-nucleus transcriptomic map of whole aging Drosophila . We characterize 163 distinct cell types and perform an in-depth analysis composition, gene expression, identities. further develop clock models to predict fly age show that ribosomal expression conserved predictive factor for age. Combining all features,...