A5031311051- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
Max Planck Institute for Terrestrial Microbiology
2024
Heidelberg University
2021-2023
Abstract The mechanisms by which viruses hijack the genetic machinery of cells they infect are current interest. When bacteriophage T4 infects Escherichia coli , it uses three different adenosine diphosphate (ADP)-ribosyltransferases (ARTs) to reprogram transcriptional and translational apparatus host ADP-ribosylation using nicotinamide adenine dinucleotide (NAD) as a substrate 1,2 . NAD has previously been identified 5′ modification cellular RNAs 3–5 Here we report that ART ModB accepts not...
Abstract Lytic bacteriophages hold substantial promise in medical and biotechnological applications. CRISPR-Cas systems offer a way to explore these mechanisms via site-specific phage mutagenesis. However, phages can resist Cas-mediated cleavage through extensive DNA modifications like cytosine glycosylation, hindering mutagenesis efficiency. Our study utilizes the eukaryotic enzyme NgTET temporarily reduce modifications, facilitating Cas nuclease enhancing This approach enables precise...
Lytic bacteriophages hold substantial promise in medical and biotechnological applications. Therefore a comprehensive understanding of phage infection mechanisms is crucial. CRISPR-Cas systems offer way to explore these via site-specific mutagenesis. However, phages can resist Cas-mediated cleavage through extensive DNA modifications like cytosine glycosylation, hindering mutagenesis efficiency. Our study utilizes the eukaryotic enzyme NgTET temporarily reduce modifications, facilitating Cas...
The mechanisms by which viruses hijack their host’s genetic machinery are of current interest. When bacteriophage T4 infects Escherichia coli , three different ARTs (ADP-ribosyltransferases) reprogram the transcriptional and translational apparatus through ADP-ribosylation using nicotinamide adenine dinucleotide (NAD) as substrate 1,2 . Recently, NAD was identified a 5’-modification cellular RNAs 3–5 Here, we report that ART ModB accepts not only but also NAD-capped RNA (NAD-RNA) attaches...
Abstract The mechanisms by which viruses hijack their host’s genetic machinery are of enormous current interest. One mechanism is adenosine diphosphate (ADP) ribosylation, where ADP-ribosyltransferases (ARTs) transfer an ADP-ribose fragment from the ubiquitous coenzyme nicotinamide adenine dinucleotide (NAD) to acceptor proteins. When bacteriophage T4 infects Escherichia coli, three different ARTs reprogram transcriptional and translational apparatus. Recently, NAD was identified as a...