A5012039249- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Antimicrobial Peptides and Activities
University of New Mexico
2022
Ludwig-Maximilians-Universität München
2012
University of Göttingen
2006-2010
Karlsruhe Institute of Technology
2009
ABSTRACT The regulatory network of the cell envelope stress response in Bacillus subtilis involves both extracytoplasmic function σ-factors and two-component signal transducing systems. One such system, LiaRS, responds to wall antibiotics that interfere with undecaprenol cycle perturbation cytoplasmic membrane. It is encoded by last two genes liaIHGFSR locus. Here, we analyzed expression LiaR-dependent operons, yhcYZ-yhdA , characterized a palindromic sequence required for activation. Since...
ABSTRACT The Lia system, a cell envelope stress response module of Bacillus subtilis , is comprised the LiaRS two-component system and membrane-anchored inhibitor protein, LiaF. It highly conserved in Firmicutes bacteria, all orthologs investigated so far are activated by wall antibiotics. In to stress, systems cocci induce expression number genes that involved conferring resistance against its inducers. contrast, complete picture LiaR regulon B. still missing no phenotypes could be...
The related lipo(depsi)peptide antibiotics daptomycin and friulimicin B show great potential in the treatment of multiply resistant gram-positive pathogens. Applying genome-wide in-depth expression profiling, we compared respective stress responses Bacillus subtilis. Both target envelope integrity, based on strong induction extracytoplasmic function sigma factor-dependent gene expression. cell stress-sensing two-component system LiaRS is exclusively strongly induced by daptomycin, indicative...
The response regulator/histidine kinase pair LiaRS of Bacillus subtilis, together with its membrane-bound inhibitor protein LiaF, constitutes an envelope stress-sensing module that is conserved in Firmicutes bacteria. LiaR positively autoregulates the expression liaIH-liaGFSR operon from a strictly LiaR-dependent promoter (P(liaI) ). A comprehensive perturbation analysis revealed functionality LiaFSR system very susceptible to alterations composition and amounts. genetic indicates...
Maintaining envelope integrity is crucial for the survival of any bacterial cell, especially those living in a complex and ever-changing habitat such as soil ecosystem. The LiaRS two-component system part regulatory network orchestrating cell-envelope stress response Bacillus subtilis. It responds to perturbations cell envelope, presence antibiotics that interfere with lipid II cycle, bacitracin or vancomycin. LiaRS-dependent regulation strictly repressed by membrane protein LiaF absence...
Staphylococcus aureus is an opportunistic, pathogenic bacteria that causes significant morbidity and mortality. As antibiotic resistance by S. continues to be a serious concern, developing novel drug therapies combat these infections vital. Quorum sensing inhibitors (QSI) dampen virulence facilitate clearance the host immune system blocking quorum signaling promotes upregulation of genes controlled accessory gene regulator (agr) operon. While QSIs have shown therapeutic promise in mouse...