A5023727914- Bone Metabolism and Diseases
- Heat shock proteins research
- Retinoids in leukemia and cellular processes
- NF-κB Signaling Pathways
- Inflammatory mediators and NSAID effects
- Endoplasmic Reticulum Stress and Disease
- Cytokine Signaling Pathways and Interactions
- Metabolism, Diabetes, and Cancer
- Protein Kinase Regulation and GTPase Signaling
- Tea Polyphenols and Effects
- Sirtuins and Resveratrol in Medicine
- Platelet Disorders and Treatments
- Estrogen and related hormone effects
- Bone health and treatments
- TGF-β signaling in diseases
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- Calpain Protease Function and Regulation
- Fibroblast Growth Factor Research
- Antioxidant Activity and Oxidative Stress
- Spaceflight effects on biology
- interferon and immune responses
- Bone health and osteoporosis research
- Antiplatelet Therapy and Cardiovascular Diseases
- Cancer, Hypoxia, and Metabolism
Gifu University
2016-2025
National Center for Geriatrics and Gerontology
2008-2025
Nagoya City University
2008-2022
Gifu University Hospital
2011
Interleukin (IL)-6 plays a pivotal role in variety of CNS functions such as the induction and modulation reactive astrogliosis, pathological inflammatory responses neuroprotection. Tumor necrosis factor (TNF)-α induces IL-6 release from rat C6 glioma cells through inhibitory kappa B (IκB)-nuclear (NFκB) pathway, p38 mitogen-activated protein (MAP) kinase stress-activated (SAPK)/c-Jun N-terminal (JNK). The present study investigated mechanism TNF-α-induced more detail than has previously been...
<h3>Introduction</h3> Endovascular embolization for tumors and vascular malformations has emerged as an important preoperative adjunct prior to resection. We describe the advantages of utilizing a recently released dual lumen balloon catheter ethylene vinyl alcohol copolymer, also known Onyx (ev3, Irvine, California, USA), variety head neck pathologies. <h3>Methods</h3> A retrospective review all cases Scepter C (MicroVention Inc, Tustin, USA) use in augmented was performed over 4 month...
We previously reported that (−)-epigallocatechin gallate (EGCG) in green tea alters plasma membrane organization and causes internalization of epidermal growth factor receptor (EGFR), resulting the suppression colon cancer cell growth. In present study, we investigated detailed mechanism underlying EGCG-induced downregulation EGFR SW480 cells. Prolonged exposure to EGCG caused degradation. However, required neither an ubiquitin ligase (c-Cbl) binding nor a phosphorylation at tyrosine...
We recently found that the inhibitory effect of (−)-epigallocatechin gallate (EGCG) on epidermal growth factor (EGF) binding to receptor (EGFR) is associated with alterations in lipid organization plasma membrane colon cancer cells. Since changes organizations are thought play a role trafficking several proteins, this study we examined effects EGCG cellular localization EGFR SW480 Treatment cells for 30 min as little 1 μg/ml caused decrease cell surface-associated EGFRs and was...
Abstract Microcystin‐LR is a liver tumor promoter in the okadaic acid class, group of potent inhibitors protein phosphatases 1 and 2A. Because inhibition phosphatases, microcystin‐LR induces hyperphosphorylation cellular proteins, including cytoskeletal proteins—cytokeratins 8 18—and causes morphological changes mouse hepatocytes primary culture. We studied effects carotenoids to antagonize microcystin‐LR‐induced hepatocytes. β‐Carotene (100 nM 100 μM), suppressed induced by dose‐dependent...
Retinoid X receptor alpha (RXRalpha) has emerged as an important nuclear involved in hepatocarcinogenesis, because its ligand suppresses the development of hepatocellular carcinoma (HCC) both experimental and clinical studies. We have demonstrated that phosphorylation RXRalpha at serine 260 interferes with function delays degradation cultured human HCC, leading to enhanced cellular proliferation. Here, we show normal liver nonproliferating hepatocyte cultures, is unphosphorylated highly...
Oral administration with acyclic retinoid, a synthetic vitamin A analog, for limited period of 12 months (48 weeks) prevented the development second primary hepatocellular carcinoma (HCC) and also improved survival patients who underwent curative treatments initial tumor. Following that randomized controlled study reported in 1996 1999, we have continued to follow up by medical imaging blood chemical analyses, found preventive effect retinoid lasted 199 weeks after randomization (or 151...
Acid sphingomyelinase (ASM) regulates the homeostasis of sphingolipids, including ceramides and sphingosine-1-phosphate (S1P). These sphingolipids regulate carcinogenesis proliferation, survival, apoptosis cancer cells. However, role ASM in host defense against liver metastasis remains unclear. In this study, involvement colon was examined using Asm-/- Asm+/+ mice that were inoculated with SL4 cells to produce metastatic tumors. demonstrated enhanced tumor growth reduced macrophage...
Dexmedetomidine, an α2-adrenoceptor agonist, is used as a sedative medication for criticalyl ill patients and known to exert neuroprotective effects by direct action on neurons indirect through astrocytes. Interleukin (IL)-6 plays key role in neuroinflammation, which accompanies infection, traumatic brain injury, ischemia, neurodegenerative disorders, both pro-inflammatory cytokine anti-inflammatory cytokine. Dexmedetomidine suppresses immune function. However, the of dexmedetomidine...
We have reported previously that acyclic retinoid, a synthetic retinoid X receptor α (RXRα)-ligand, suppresses the development of hepatocellular carcinoma (HCC) in patients with chronic liver disease. On other hand, HCCs become refractory to physiological concentrations natural RXRα-ligand, 9- cis retinoic acid (9cRA), due extracellular signal-regulated kinase (Erk) 1/2-mediated phosphorylation and inactivation RXRα. Here, we show restores function RXRα human HCC-derived HuH7 cells by...
Hepatocellular carcinoma (HCC) is one of the most prevalent cancers worldwide. However, effective chemopreventive and chemotherapeutic agents for this cancer have not yet been developed. In clinical trials acyclic retinoid (ACR) vitamin K 2 (VK ) decreased recurrence rate HCC. present study we examined possible combined effects ACR or another 9‐ cis retinoic acid (9cRA) plus VK in HuH7 human HCC cell line. We found that combination 1.0 µM 9cRA 10 synergistically inhibited growth cells...
Heat shock protein 27, one of the low molecular weight stress proteins, is recognized as a chaperone; however, other functions have not yet been well established. Phosphorylated heat 27 levels inversely correlate with progression human hepatocellular carcinoma. This study shows that phosphorylated interferes cell growth carcinoma-derived HuH7 cells in presence proinflammatory cytokine, tumor necrosis factor-α, via inhibition sustained activation extracellular signal-regulated kinase signal...
Human hepatocellular carcinoma (HCC) is one of the major malignancies in world. Small heat shock proteins (HSPs) are reported to play an important role regulation a variety cancer cell functions, and functions small HSPs regulated by post-translational modifications such as phosphorylation. We previously that protein levels HSP, HSP20 (HSPB6), decrease vascular invasion positive HCC compared with those negative invasion. Therefore, present study, we investigated whether implicated migration...
We investigated the relationship between HSP27 phosphorylation and collagen-stimulated activation of platelets in patients with diabetes mellitus (DM). Platelet-rich plasma was prepared from blood type 2 DM patients. The platelet aggregation analyzed size aggregates by an aggregometer using a laser scattering method. protein Western blotting. Phosphorylated-HSP27 PDGF-AB released were measured ELISA. phosphorylated-HSP27 levels at Ser-78 Ser-82 induced collagen directly proportional to...
Amyloid β (Aβ) (1‒40) is the major form in amyloid plaques, while Aβ (1‒42) predominant neuronal plaques. Anti-Aβ antibodies are clinically accepted for Alzheimer's disease treatment to remove from plaques; however, increase of intracranial hemorrhagic risk a concern. We reported that inhibits thrombin receptor-activating protein (TRAP)-induced platelet activation healthy volunteers, and responsiveness related brain atrophy diabetes mellitus (DM) patients. investigated difference between...
Acyclic retinoid, a synthetic retinoid analog, as well interferon alfa (IFN-alpha) and IFN-beta induce apoptosis in hepatocellular carcinoma (HCC) cells are used clinically the prevention of HCC. Here, we show that acyclic acts synergistically with IFNs suppressing growth inducing (as characterized by DNA fragmentation chromatin condensation) 5 human HCC cell lines (JHH7, HuH7, PLC/PRF/5, HLE, HLF). This synergism was only observed when were pretreated whereas natural retinoic acids...
We previously showed that endothelin‐1 (ET‐1) stimulates the synthesis of interleukin‐6 (IL‐6), a potent bone resorptive agent, in osteoblast‐like MC3T3‐E1 cells, and protein kinase C (PKC)‐dependent p44/p42 mitogen‐activated (MAP) plays part IL‐6 synthesis. In present study, we investigated effect (−)‐epigallocatechin gallate (EGCG), one major flavonoids containing green tea, on ET‐1‐induced osteoblasts underlying mechanism. EGCG significantly reduced stimulated by ET‐1 cells as well...