Abstract Mechanistic details of DNA compaction is essential blue print for gene regulation in living organisms. Many vitro studies have been implemented using several agents. However, these compacting agents may some kinds cytotoxic effects to the cells. To minimize this aspect, research works had performed, but people never focused green solvent, i.e. room temperature ionic liquid as agent. best our knowledge, first ever report where we shown that guanidinium...

Host–guest interactions between cucurbit[7]uril (CB7) and a cardiotonic drug, milrinone, have been explored using steady state pico-second time-resolved techniques. A novel fluorescence switch from ultraviolet (UV) to visible (cyan) is observed as consequence of upward pKa shift the drug inside nano-cavity cucurbit[7]uril.

Graphene oxide based molecular switching of ellipticine (E) has been utilized to probe its efficient loading onto graphene (GO) and subsequent release intra-cellular biomolecules like DNA/RNA. The green fluorescence E switches blue in GO back with polynucleotides. intensified emission the ellipticine-GO (E-GO) complex human serum albumin (HSA), a bluish upon addition dsDNA. Electron microscopy reveals formation distinctive 3D assemblies involving biomolecule(s) probably through non-covalent...

Herein, we have reported a new strategy for the synthesis of highly stable fluorescent copper nanoclusters (CuNCs) with l-cysteine (Cys) as protecting ligand within water nanopool reverse micelles (RMs). In present work, efforts are also given to address origin excitation-dependent fluorescence spectral shift CuNCs. From our experiments, elucidated that broad from CuNCs in RMs consists two spectrally overlapped bands corresponding metal-core and surface states The intrinsic emission...

The G-quadruplex (GQ-DNA), an alternative structure motif of DNA, has emerged as a novel and exciting target for anticancer drug discovery. GQ-DNA formed in the presence monovalent cations (Na(+)/K(+)) by human telomeric DNA is point interest due to their direct relevance cellular aging abnormal cell growths. Small molecules that selectively stabilize structures are considered be potential therapeutic agents. Herein, we probe proflavine (a well-known intercalator, hence acting...

Abstract The effect of cucurbit[7]uril (CB[7]) nano‐caging on the photophysical properties, particularly excited‐state proton transfer (ESPT) reaction, an eminent anti‐cancer drug, topotecan (TPT), is demonstrated through steady‐state and time‐resolved fluorescence measurements. TPT in water (pH 6) exists exclusively as cationic form (C) ground state. However, drug emission mainly comes from zwitterionic (Z*) TPT, attributed to water‐assisted ESPT between 10‐hydroxyl group water, which leads...

Mesoporous silica nano-channel (MCM-41) based molecular switching of a biologically important anticancer drug, namely, ellipticine (EPT) has been utilized to probe its efficient loading onto MCM-41, and subsequent release intra-cellular biomolecules, like DNA. By exploiting various spectroscopic techniques (like, steady state fluorescence, time-resolved fluorescence circular dichroism), it shown that EPT can be easily translocated from MCM-41 DNA without using any external stimulant. Blue...

Host–guest interactions between an anticancer drug, ellipticine (EPT), and molecular containers (cucurbitruils (CBn) cyclodextrins (CD)) are investigated with the help of steady state time-resolved fluorescence measurements. Our experimental results confirm formation 1:1 inclusion complexes CB7 CB8. The protonated form EPT predominantly prevails in due to stabilization achieved through ion–dipole interaction host positively charged drug. Drug does not complex CB6, which is smaller cavity...

Prototropical and photophysical properties of an anticancer drug, ellipticine, are explored inside the octyl-β-<sc>d</sc>-glucoside micelles using steady state time-resolved fluorescence spectroscopic techniques.

Here, we investigate the effect of urea in unfolding dynamics flavin adenine dinucleotide (FAD), an important enzymatic cofactor, through steady state, time-resolved fluorescence spectroscopic and molecular (MD) simulation studies. Steady state results indicate possibility induced FAD, inferred from increasing emission intensity FAD with urea. The TCSPC up-conversion suggest that stack–unstack severely gets affected presence leads to increase unstack conformation population 15% pure water...

Femtosecond fluorescence upconversion measurements are employed to elucidate the mechanism of ultrafast double proton transfer dynamics BP(OH)2 inside molecular containers (cucurbit[7]uril (CB7) and β-cyclodextrin (β-CD)). up-converted signals in water consist growth followed by a long decay component (~650 ps). The appearance (~35 ps) signal indicates presence two-step sequential process water. Surprisingly, CB7 nano-cavity does not exhibit any characteristic process. Interestingly, exists...

A visible fluorescence switch of an eminent anti-carcinogen, ellipticine has been used to probe non-specific protein-DNA interaction. The unique pattern complexation is depicted for the first time through field emission scanning electron microscopy (FE-SEM) images and spectroscopic techniques.

Herein, we explored the photophysical properties of antimalarial, anticancer drug cryptolepine (CRYP) in presence macrocyclic host cucurbit[7]uril (CB7) and DNA with help steady-state time-resolved fluorescence techniques. Ground-state excited-state calculations based on density functional theory were also performed to obtain insight into shape, electron distribution, energetics molecular orbitals CRYP. CRYP exists two forms depending pH medium, namely, a cationic (charge transfer) form...

Reverse hexagonal (HII) liquid crystalline material based on glycerol monooleate (GMO) is considered as a potential carrier for drugs and other important biomolecules due to its thermotropic phase change excellent morphology. In this work, the dynamics of encapsulated water, which plays role in stabilization formation reverse mesophase, has been investigated by time dependent Stokes shift method using Coumarin-343 solvation probe. The mesophase transformation L2 have monitored small-angle...

Controlling luminescence behaviors of organic chromophores is essential for their applications. As emissions from are strongly modulated by structural fluctuations and external environments, comprehensive understandings remain elusive. Here, we demonstrate the modulation photophysics benzophenone (Bzp), a prototypical triplet sensor molecule, encapsulating it into mesoporous silica nanomaterials (MSNs). We systematically probed photoluminescence property using time-resolved (TR-PL)...

The excited state proton transfer (ESPT) dynamics of a potentially important anticancer drug, Topotecan (TPT), has been explored in aqueous reverse micelle (RM) using steady-state and time-resolved fluorescence measurements. Both the emission spectrum area normalized infer generation zwitterionic form TPT from cationic TPT, as result ESPT process −OH group to nearby water molecule. were found be severely retarded inside nanocavities RMs, yielding time constants 250 ps 1.0 ns, which is...

The modulation of kinetics and pathways in the ESIPT process proton transfer probes holds significant potential for advancing applications bio-imaging, drug delivery, OLEDs. One effective approach achieving this is altering H-bonding donating capability surrounding medium. To investigate this, we conducted a comprehensive study on excited state intramolecular double [2,2′-bipyridyl]-3,3′-diol (BP(OH)2) within confined spaces silica nanochannels, namely, MCM-41. MCM-41, known its versatile...

Excited-state intramolecular proton transfer (ESIPT) reactions are crucial in photoresponsive materials and fluorescent markers. The compound 4-aminophthalimide (4-AP) has been reported to exhibit solvent-assisted ESIPT protic solvents, such as methanol, wherein the solvent interacts with 4-AP form a six-membered hydrogen-bonded ring that is strengthened upon excitation. Although controversial observation of extensively studied, molecular mechanism yet be fully explored. In this study,...