A5020076769- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Cancer-related gene regulation
- Ferroptosis and cancer prognosis
- RNA Interference and Gene Delivery
- Signaling Pathways in Disease
- Heat shock proteins research
- Caveolin-1 and cellular processes
- Connexins and lens biology
- Machine Learning in Bioinformatics
- Prostate Cancer Treatment and Research
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Cholesterol and Lipid Metabolism
- Lipid Membrane Structure and Behavior
- Cancer Research and Treatments
- Hippo pathway signaling and YAP/TAZ
- Cellular transport and secretion
- Cancer, Hypoxia, and Metabolism
- Complement system in diseases
- Biomedical Research and Pathophysiology
- S100 Proteins and Annexins
- Immune cells in cancer
University of Nebraska Medical Center
2011-2023
Abstract Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature monocytes and granulocytes that potent inhibitors T cell activation. A role for MDSCs in bacterial infections has only recently emerged, nothing is known about MDSC function the context Staphylococcus aureus infection. Because S. biofilms capable subverting immune-mediated clearance, we examined whether could play this process. CD11b+Gr-1+ represented main cellular infiltrate during orthopedic...
Abstract Prostate cancer progression is associated with upregulation of sialyl-T antigen produced by β-galactoside α-2,3-sialyltransferase-1 (ST3Gal1) but not core 2-associated polylactosamine despite expression 2 N-acetylglucosaminyltransferase-L (C2GnT-L/GCNT1). This property allows androgen-refractory prostate cells to evade galectin-1 (LGALS1)–induced apoptosis, the mechanism known. We have recently reported that Golgi targeting glycosyltransferases mediated golgins: giantin (GOLGB1) for...
Eps15 Homology Domain-containing 3 (EHD3), a member of the EHD protein family that regulates endocytic recycling, is first reported to be specifically expressed in glomerular endothelium kidney; therefore we generated Ehd3–/– mice and assessed renal development pathology. animals showed no overt defects, exhibited proteinuria or However, as expression EHD4, related member, was elevated suggested functional compensation, analyzed Ehd3–/–; Ehd4–/– mice. These were smaller, possessed smaller...
Abstract Prostate cancer progression to the lethal metastatic castration-resistant phenotype (mCRPC) is driven by αv integrins and associated with Golgi disorganization activation of ATF6 branch unfolded protein response (UPR). Overexpression requires N-acetylglucosaminyltransferase-V (MGAT5)-mediated glycosylation subsequent cluster formation Galectin-3 (Gal-3). However, mechanism underlying this altered missing. For first time, using HALO analysis IHC, we found a strong association...
Microvillous inclusion disease (MVID) is a congenital condition presenting with intractable diarrhea. Biopsies demonstrate abnormal apical PAS and CD10 staining in surface enterocytes correlating the presence of characteristic cytoplasmic inclusions. MVID has been linked to mutations myosin Vb, important membrane recycling. Rab11 associates Vb vesicle membranes also integral recycling plasma components. The authors performed immunostaining on biopsies from 7 cases, 10 normal small...
Background It is known that ethanol (EtOH) and its metabolites have a negative effect on protein glycosylation. The fragmentation of the Golgi apparatus induced by alteration structure largest matrix protein, giantin, major consequence damaging effects Et OH ‐metabolism Golgi; however, link between this abnormal glycosylation remains unknown. Because previously we shown morphology dictates glycosylation, examined administration has function residential enzymes involved in N ‐glycosylation....
Previous work from our laboratories has identified multiple defects in endocytosis, protein trafficking, and secretion, along with altered Golgi function after alcohol administration. Manifestation of alcohol‐associated liver disease (ALD) is associated an aberrant several hepatic proteins, including asialoglycoprotein receptor (ASGP‐R), their atypical distribution at the plasma membrane (PM), secretion abnormally glycosylated forms into bloodstream, but trafficking mechanism unknown. Here...
<p>Supplemental Movie 1 PC-3 cell treated with water, stained for Golgi (GRASP65, green) and MGAT3 (red). Surfaces were reconstructed by Imaris. Supplemental 2 HCQ, 3 Integrin αv (red), Gal-3 (green), EEA1 (magenta). Areas of 3-color colocalization are highlighted white. 4 Imaris.</p>
<p>Supplementary Methods and Materials</p>
<p>Figure S1. (A) Representative images of triple IHC staining Integrin αvβ3 (green), Gal-3 (red), and E-cadherin (brown) in normal prostate tumor tissues from PCa patients with different grades. Deconvoluted are shown on the right; bars, 50 µm. (B) Quantification H-score at PM samples A. Dunn Test (1964) Kruskal-Wallis multiple comparisons, p-adjusted using Benjamini-Hochberg; ****p<0.0001 *p<0.05, median ± SD. (C) colocalization grades 2-5. test; mean SD.</p>
<p>PC-3 cell treated with HCQ, stained for Integrin αv (red), Gal-3 (green), EEA1 (magenta). Areas of 3-color colocalization are highlighted white. Surfaces were reconstructed by Imaris.</p>
<p>Figure S10. (A) Evaluation of MGAT5-mediated Integrin αv glycosylation in the orthotopic tumor samples. Tissue samples were co-stained with (green) and Cy3 labeled PHA-L lectin (red). Representative Z-stack images collected by SIM reconstructed using Imaris for clarity. (B) Mander's colocalization between red green from A. Kruskal-Wallis test; n indicates number foci counted. For all statistics: median ± SD, ****p<0.0001, ***p<0.001, **p<0.005, *p<0.05. (C)...
<p>Figure S5. (A, B) Representative EM images of PC-3 (A) and DU145 (B) cells. Note phagophore nucleation from Golgi (arrowheads) autophagosomes localizing close to the (arrow). Orange boxes indicate areas magnified at right. (C) WIPI2 W-B fractions isolated LNCaP, PC-3, Samples were normalized by GM130.</p>
<p>Figure S4. (A) Representative images of Golgi in DU145 cells treated with 60 µM HCQ for 72 h and stained by GM130 (green); bars, 20 µm. (B) Quantification spots per cell samples from A. Unpaired t test; **p<0.01, mean ± SD; n represents a number counted. (C) control or ATF6α siRNAs (D) C. ***p<0.001, (E) ATF6 W-B the lysates transfected siRNAs; β-actin is loading control. (F) PC-3 ATG5 (G) F. ****p<0.0001, (H) γ-tubulin (I) (J) I. (K) (L) Bafilomycin A1 (10 h) appropriate...
<p>Figure S8. AST (A) and ALT (B) serum activity from the indicated mice. were examined using Assay Kit (Abcam, ab241035) ab105135), respectively, per manufacturer's protocol. Kruskal-Wallis test, all p>0.05, mean ± SD; n represents number of mice tested.</p>
<p>PC-3 cell treated with water, stained for Golgi (GRASP65, green) and MGAT3 (red). Surfaces were reconstructed by Imaris.</p>
<p>PC-3 cell treated with water, stained for Integrin αv (red), Gal-3 (green), EEA1 (magenta). Areas of 3-color colocalization are highlighted white. Surfaces were reconstructed by Imaris.</p>
<p>Figure S6. (A) Immunostaining of Gal-3 (green) and Na+/K+-ATPase (red) in control HCQ-treated PC-3 cells; bars, 20 µm. (B) Quantification integrated intensity at PM cells from A. Mann Whitney test; *p<0.05, mean ± SD; n indicates number cells.</p>
<p>Figure S3. (A) Representative images of triple IHC staining Integrin αvβ6 (green), Gal-3 (brown), and Na+/K+-ATPase (red) in normal prostate tumor tissues from PCa patients with different grades. Deconvoluted are shown on the right; bars, 100 µm. (B) Quantification H-score at PM samples A. Dunn Test (1964) Kruskal-Wallis multiple comparisons, p-adjusted using Benjamini-Hochberg; ****p<0.0001 **p<0.01, median ± SD. (C) colocalization PCa, grades 2-5. Mann Whitney test;...
<p>Figure S2. (A) Representative images of triple IHC staining Integrin αvβ5 (green), Gal-3 (red), and E-cadherin (brown) in the normal prostate tumor tissues from PCa patients with different grades. Deconvoluted are shown on right; bars, 100 µm. (B) Quantification H-score at PM samples A. Dunn Test (1964) Kruskal-Wallis multiple comparisons, p-adjusted using Benjamini-Hochberg; ****p<0.0001 ***p<0.001, median ± SD. (C) colocalization PCa, grades 2-5. test; mean SD.</p>
<p>PC-3 cell treated with HCQ, stained for Golgi (GRASP65, green) and MGAT3 (red). Surfaces were reconstructed by Imaris.</p>
<p>Figure S9. (A) Left Panels: representative images of triple IHC staining Na+/K+-ATPase (green), Integrin αv (red), and Gal-3 (brown) in the orthotopic tumor samples from indicated group mice; bars, 100 µm. Middle panel: IF Golgi stained by GM130 prostate foci; 10 White boxes indicate areas enlarged at right. (B) Quantification fragments per cell foci A; median ± SD. (C) ATF6 20 Areas mice epithelium is highlighted dotted lines. (D) intensity C; For all graphs: pairwise comparisons...