A5092434324- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Advanced Electron Microscopy Techniques and Applications
ETH Zurich
2023
Fragment-based drug discovery has emerged as a powerful approach for developing therapeutics against challenging targets, including the GTPase KRAS. Here, we report an NMR-based screening campaign employing state-of-the-art techniques to evaluate library of 890 fragments oncogenic KRAS G12V mutant bound GMP-PNP. Further HSQC titration experiments identified hits with low millimolar affinities binding within SI/SII switch region, which forms interface effector proteins. To elucidate modes,...
This study provides proof of charge complementarity-based inclusion complex formation between an engineered protein nanocage and otherwise degradation-prone cargo in live bacterial cells.