A5063004071- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Computational Drug Discovery Methods
- Microbial Natural Products and Biosynthesis
- Near-Field Optical Microscopy
- Weed Control and Herbicide Applications
- Synthesis and biological activity
- Synthesis of Organic Compounds
- Fungal Plant Pathogen Control
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- vaccines and immunoinformatics approaches
- Pesticide and Herbicide Environmental Studies
- Microbial Metabolic Engineering and Bioproduction
Fundación Ciencias Exactas y Naturales
2022-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2022-2025
University of Buenos Aires
2022-2025
Universidad de Extremadura
2023
Bacterial P450 cytochromes (BacCYPs) are versatile heme-containing proteins responsible for oxidation reactions on a wide range of substrates, contributing to the production valuable natural products with limitless biotechnological potential. While sequencing microbial genomes has provided wealth BacCYP sequences, functional characterization lags behind, hindering our understanding their roles. This study employs comprehensive approach predict substrate specificity, bridging gap between...
Abstract Fluorescence nanoscopy and single‐molecule methods are entering the realm of structural biology, breaking new ground for dynamic measurements at room temperature liquid environments. Here, localization microscopy, polarization‐dependent excitation, protein engineering combined to determine orientation a fluorophore forming hydrogen bonds inside cavity. The observed conformations in good agreement with molecular dynamics simulations, enabling new, more realistic interplay between...
Phenotypic screening is a powerful technique that allowed the discovery of antimicrobials to fight infectious diseases considered deadly less than century ago. In high throughput phenotypic assays, thousands compounds are tested for their capacity inhibit microbial growth in-vitro . After an active compound found, identifying molecular target next step. Knowing specific key understanding its mechanism action, and essential future drug development. Moreover, this knowledge allows developers...
This paper reports the preparation and herbicidal evaluation of a small library acylhydrazones based on synthetic herbicide metribuzin. The hydrazone linkage easily obtained by reaction metribuzin with aliphatic aromatic aldehydes, masks efficiently exocyclic amino group, thereby altering significantly H-bonding receptor increasing lipophilicity relative to parent herbicide. structures all compounds, including key stereochemical issues conformation E/Z configuration around CN bond were...
This paper reports the preparation and herbicidal evaluation of a small library acylhydrazones based on synthetic herbicide metribuzin. The hydrazone linkage easily obtained by reaction metribuzin with aliphatic aromatic aldehydes, masks efficiently exocyclic amino group, thereby altering significantly H-bonding receptor increasing lipophilicity relative to parent herbicide. structures all compounds, including key stereochemical issues conformation E/Z configuration around C=N bond were...