A5045865002- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Genetic diversity and population structure
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- RNA modifications and cancer
- T-cell and B-cell Immunology
- Bacterial Identification and Susceptibility Testing
- RNA regulation and disease
- Streptococcal Infections and Treatments
- Primate Behavior and Ecology
- Plant and Fungal Species Descriptions
- Yeasts and Rust Fungi Studies
- Antimicrobial Resistance in Staphylococcus
- Infections and bacterial resistance
- Plant and fungal interactions
- Molecular Biology Techniques and Applications
- Safety Systems Engineering in Autonomy
- Forensic and Genetic Research
- MicroRNA in disease regulation
- Mycobacterium research and diagnosis
- Plant Pathogens and Fungal Diseases
- Cancer-related molecular mechanisms research
- Plant and animal studies
- Antibiotic Resistance in Bacteria
Max Planck Unit for the Science of Pathogens
2021-2024
Charité - Universitätsmedizin Berlin
2024
Max Planck Society
2007-2021
University Hospital Leipzig
2013-2017
Leipzig University
2013-2017
Adaptive Biotechnologies (United States)
2014-2015
Max Planck Institute for Evolutionary Anthropology
2007-2013
The chromosome 9p21 (Chr9p21) locus of coronary artery disease has been identified in the first surge genome-wide association and is strongest genetic factor atherosclerosis known today. Chr9p21 encodes long non-coding RNA (ncRNA) antisense INK4 (ANRIL). ANRIL expression associated with genotype correlated severity. Here, we report on molecular mechanisms through which regulates target-genes trans, leading to increased cell proliferation, adhesion decreased apoptosis, are all essential...
Primates, the mammalian order including our own species, comprise 480 species in 78 genera. Thus, they represent third largest of 18 orders eutherian mammals. Although recent phylogenetic studies on primates are increasingly built molecular datasets, most these have focused taxonomic subgroups within order. Complete mitochondrial (mt) genomes proven to be extremely useful deciphering within-order relationships even up deep nodes. Using 454 sequencing, we sequenced 32 new complete mt adding...
Guenons (tribe Cercopithecini) are one of the most diverse groups primates.They occupy all sub-Saharan Africa and show great variation in ecology, behavior, morphology.This led to description over 60 species subspecies.Here, using next-generation DNA sequencing (NGS) combination with targeted capture, we sequenced 92 mitochondrial genomes from museum-preserved specimens as old 117 years.We infer evolutionary relationships estimate divergence times almost guenon taxa based on genome...
Because of the time and cost associated with Sanger sequencing complete human mtDNA genomes, practically all evolutionary studies have screened samples first to define haplogroups then either selected a few from each haplogroup, or many particular haplogroup interest, for genome sequencing. Such biased sampling precludes analyses interest. Here, we used high-throughput platforms generate, rapidly inexpensively, 109 sequences random individuals three Filipino groups, including one Negrito...
Abstract Adenosine-to-inosine (A-to-I) RNA editing plays an important role in the post-transcriptional regulation of eukaryotic cell physiology. However, our understanding occurrence, function and A-to-I bacteria remains limited. Bacterial mRNA is catalysed by deaminase TadA, which was originally described to modify a single tRNA Escherichia coli. Intriguingly, several bacterial species appear perform on more than one tRNA. Here, we provide evidence that human pathogen Streptococcus...
Genetic tools form the basis for study of molecular mechanisms. Despite many recent advances in field genetic engineering bacteria, toolsets remain scarce non-model organisms, such as obligatory human pathogen Streptococcus pyogenes. To overcome this limitation and enable straightforward investigation gene functions S. pyogenes , we have developed a comprehensive toolset. By adapting combining different previously applied other Gram-positive created new replicative integrative plasmids...
Abstract Birds-of-paradise represent a textbook example for geographical speciation and sexual selection. Perhaps the most iconic genus is Paradisaea, which restricted to New Guinea few surrounding islands. Although several species concepts have been applied in past disentangle different entities within this genus, no attempt has made so far uncover phylogeographical patterns based on genetic dataset that includes multiple individuals per species. Here, we amplicon sequencing mitochondrial...
ABSTRACT Adenosine-to-inosine (A-to-I) RNA editing plays an important role in the post-transcriptional regulation of eukaryotic cell physiology. However, our understanding occurrence, function and A-to-I bacteria remains limited. Bacterial mRNA is catalysed by deaminase TadA, which was originally described to modify a single tRNA E. coli . Intriguingly, several bacterial species appear perform on more than one tRNA. Here, we provide evidence that human pathogen Streptococcus pyogenes , has...
Abstract Genetic tools form the basis for study of molecular mechanisms. Despite many recent advances in field genetic engineering bacteria, toolsets remain scarce non-model organisms, such as obligatory human pathogen Streptococcus pyogenes . In this study, we set out to develop a comprehensive plasmids, promoters and reporters S. We present an expansion current toolbox that comprises new replicative site-specific integrative plasmids. Moreover, established collection constitutive with wide...
Although CMV reactivation has long been implicated in post-transplant immune dysfunction, the molecular mechanisms by which even a single event can increase risk of transplant-related mortality remains mystery. To address this, we combined multiparameter flow cytometric analysis and T cell subpopulation sorting with high-throughput sequencing repertoire, to produce an exhaustive evaluation impact on reconstitution after BMT. These studies were performed initial cohort 17 transplant patients,...
Rationale: In BMT, CMV reactivation is linked to both an increase in immune-mediated complications and, paradoxically, a reduced risk of malignant relapse. The purpose this study was determine the mechanisms by which induces dichotomous effects on post-transplant immunity. Methods: Patients from two IRB-approved BMT studies (n=17) were studied with multiparameter flow cytometry, CMV-specific tetramers and TCR deep sequencing, immune reconstitution reactivating (+CMV) non-reactivating (-CMV)...