Previous research has suggested that human herpesvirus-6 (HHV-6) may integrate into host cell chromosomes and be vertically transmitted in the germ line, but evidence—primarily fluorescence situ hybridization (FISH)—is indirect. We sought, first, to definitively test these two hypotheses. Peripheral blood mononuclear cells (PBMCs) were isolated from families which several members, including at least one parent child, had unusually high copy numbers of HHV-6 DNA per milliliter blood. FISH...

Rheumatoid arthritis is associated with the HLA antigen HLA-DR4. Disease susceptibility maps to amino acid sequence QKRAA located in third hypervariable region of DR beta-1 chain. This thought be a site recognition for T-cell receptor. We searched an human environment that could induce this sequence. An analysis protein and DNA databases revealed Epstein-Barr virus glycoprotein gp110, which encoded by BALF4 open reading frame, contains QKRAAQRAA, highly homologous rheumatoid determinant....

When the latent Epstein-Barr virus (EBV) genome in B95-8 cells is induced into a replicative phase, two abundant early RNAs are transcribed rightward from EBV BamHI H DNA fragment F. Analysis of cDNA clones prepared RNA replicating revealed that both contain BHRF1 open reading frame. Part BHRF1, cloned prokaryotic fusion protein expression vector, expressed Escherichia coli and purified was used to generate monoclonal antibody against BHRF1. This then employed characterize encoded by EBV....

Sodium butyrate induces the Epstein-Barr virus cycle in latently infected P3HR-1 cells with a high efficiency. This fact was utilized for metabolic labeling of antigens. Nonproducer Raji cells, lacking both early antigen and viral capsid antigen, were used as controls. Immunoprecipitation patterns compared 13 anti-Epstein-Barr (viral antigen) - positive 3 negative sera. Sixteen polypeptides identified being associated lytic cycle. Their molecular weights ranged from 31,000 (31K) to 275K on...

The Epstein-Barr virus (EBV) causes infectious mononucleosis and is linked to several disparate malignancies. Prior studies on patients with multiple sclerosis (MS) showed that 100% are EBV-seropositive their blood contains higher antibody titers than those of controls both transformation lytic cycle antigens. We performed three different assays for antibodies in CSF major EBV antigens from MS controls. Among 93 MS, 79 (85%) had reacted a 70 kD protein, shown be the nuclear antigen, EBNA-1,...

A monoclonal antibody, selected for reactivity with the Epstein-Barr virus (EBV)-encoded antigen EBNA-1, exhibited strong synovial lining cells in joint biopsies from 10 of 12 patients rheumatoid arthritis (RA) and adherent eluted these tissues. No staining RA membrane frozen tissue sections or synovial-lining was obtained antibodies directed against other EBV-encoded antigens (anti-p160, anti-gp200/350) encoded by cytomegalovirus, herpes simplex viruses, human T cell leukemia type I. Among...

The Epstein-Barr virus-determined nuclear antigen (EBNA) was purified 700-fold to apparent homogeneity from Raji and Namalwa cell extracts by a three-step procedure involving heat treatment, DNA-cellulose chromatography, hydroxyapatite chromatography. Acid-fixed binding complement fixation were used monitor antigenic specificity. Purified EBNA also capable of specifically inhibiting the regular anticomplement immunofluorescence reaction for against target cells. had molecular weight 170,000...

A soluble complement-fixing antigen carried by Epstein-Barr virus (EBV)-transformed human cells has been previously extracted from cell nuclei and purified DNA-cellulose chromatography [Luka, J., Siegert, W. & Klein, G. (1977) J. Virol., in press]. On addition of this to methanol/acetic acid-fixed metaphase chrmosomes, followed exposure sera containing antibodies against the EBV-determined nuclear (EBNA), brilliant positive staining was obtained anti-complement immunofluorescence. There...

The Eptstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolate nuclei of two EBV-transformed cell lines- Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied DNA-cellulose chromatography a 51Cr release complement fixation assay. EBNA binds to both double-stranded single-stranded calf thymus DNA, showing higher affinity DNA. There no detectable difference in the DNA binding prepared AW-Ramos cells.

The Epstein-Barr virus-determined nuclear antigen (EBNA) was purified from extracts of the human lymphoid cell lines Raji, Namalwa, and B95-8/MLD by two different methods. In first approach, apparently native 1,200-fold a four-step procedure involving DNA-cellulose chromatography, blue dexptran-agarose hydroxyapatite gel filtration, employing complement fixation as assay procedure. Such EBNA preparations specifically inhibited anticomplement immunofluorescence test for bound to...

Fungal infections are a global problem imposing considerable disease burden. One of the unmet needs in addressing these is rapid, sensitive diagnostics. A promising molecular diagnostic approach high-resolution melt analysis (HRM). However, there has been little effort leveraging HRM data for automated, objective identification fungal species. The purpose studies was to assess utility distance methods developed comparison time series classify curves as means species identification. Dynamic...

Abstract Background: Human herpesvirus-7 (HHV-7) is a ubiquitous, CD4+ T cell lymphotropic virus. It establishes lifelong latency and may reactivate, particularly in immunocompromised individuals. We had previously detected the presence of HHV-7 two-thirds angiosarcoma clinical samples. HHV-7-positive (HHV-7+) tumors were shown to be relatively enriched for B- mast cells high tumor inflammation signature scores compared HHV-7-negative tumors. Objective: To investigate pathogenesis host its...

BACKGROUND: CMV DNA screening may be a useful adjunct to serologic tests in distinguishing potentially infectious blood donations from those that are “CMV‐safe.” However, there is currently no consensus on the optimal assay method for accurate detection of donors. STUDY DESIGN AND METHODS: A blinded multicenter evaluation seven PCR assays was performed by five laboratories using coded sets analytical controls and donor samples. RESULTS: Five displayed sufficient sensitivity screening, as...

We examined the expression of Epstein-Barr virus (EBV)-induced proteins (LMP [latent membrane protein], EBNA-2, and CD23) a lytic protein, viral capsid antigen (VCA), in five acquired immune deficiency syndrome (AIDS)-related primary CNS lymphomas (PCNSLs). compared that with same PCNSL from six immunocompetent patients severe combined (SCID) mouse brains injected EBV-infected lymphoblastoid cell lines (LCLs). Brain biopsy tissue an AIDS patient progressive multifocal leukoencephalopathy...

A monoclonal antibody designated V3 was produced against a late protein associated with the Epstein-Barr virus-induced viral capsid antigen complex. The reacted discrete patches in nuclei of infected cells as well virus particles, shown by immunofluorescence and ultrastructural immunoperoxidase staining. molecular weight precipitated this ca. 160,000. All anti-viral antibody-positive sera tested an enzyme-linked immunosorbent assay purified protein. synthesis inhibited phosphonoacetic acid...

A 62,000-dalton (62K) cell protein reacts with antisera to the 72K polypeptide of Epstein-Barr virus nuclear antigen (EBNA) in immunoblots. This was initially detected EBNA-negative as well EBNA-positive lines anti-EBNA-positive human sera. monoclonal antibody raised against EBNA and an antiserum from a rabbit immunized glycine-alanine domain also reacted cellular protein. The partially purified genome-positive -negative lines. Absorption experiments identified shared antigenic determinant...

The Epstein-Barr virus DNA polymerase was purified from extracts of P3HR-1 cells treated with n-butyrate for induction the viral cycle. Sequential chromatography on cellulose, phosphocellulose, and blue Sepharose yielded an enzyme preparation more than 1,300-fold. distinct cellular enzymes but resembled in infected herpes simplex type 1 or 2. active had apparent molecular weight 185,000 as estimated by gel filtration Sephacryl S-300. Sodium dodecyl sulfate-polyacrylamide electrophoresis...

Abstract We assessed 33 lymphoid tissues from 15 patients, including 7 with X‐linked lymphoproliferative disease (XLP) and 8 pa tients sporadic fatal infectious mononucleosis (IM), to determine whether the cellular infiltrate had immu nophenotype expressed Epstein‐Barr virus (EBV)‐encoded proteins characteristic of either EBV‐immortalized lymphoblastoid cell lines (LCL) or EBV‐carrying Burkitt lymphoma (BL) cells. The results these studies revealed that in 13 cases proliferating B cells were...

Abstract In agreement with the findings of previous authors, we could not detect a virally determined nuclear antigen in Herpesvirus papio (HVP)‐transformed baboon lymphoid lines by anticomplementary staining situ , as for EBNA. However, means our recently developed acid‐fixed binding technique an EBNA‐like be readily demonstrated, after extraction from both producer and non‐producer lines. We propose to designate HUPNA. It can detected human anti‐EBNA antibody, suggesting cross‐reactivity,...

Abstract Human herpesvirus‐6 (HHV‐6)A and 6B are ubiquitous betaherpesviruses viruses with lymphotropic neurotropic potential. As reported earlier, these establish latency by integration into the telomeres of host chromosomes. Chromosomally integrated HHV‐6 (CIHHV‐6) can be transmitted vertically from parent to child. Some CIHHV‐6 patients suffering neurological symptoms, while others remain asymptomatic. Four CNS dysfunction were treated valganciclovir or foscarnet. replication was detected...