A5035661249- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Protein Degradation and Inhibitors
- RNA Research and Splicing
- Acute Myeloid Leukemia Research
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Hippo pathway signaling and YAP/TAZ
- Mitochondrial Function and Pathology
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Cytokine Signaling Pathways and Interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Caveolin-1 and cellular processes
- Single-cell and spatial transcriptomics
University of Pennsylvania
2021-2024
National Research Council Canada
2018
Abstract Epigenetic programs are dysregulated in acute myeloid leukemia (AML) and help enforce an oncogenic state of differentiation arrest. To identify key epigenetic regulators AML cell fate, we performed a differentiation-focused CRISPR screen cells. This identified the histone acetyltransferase KAT6A as novel regulator that drives critical leukemogenic gene-expression programs. We show is initiator newly described transcriptional control module which KAT6A-catalyzed promoter H3K9ac bound...
Stem and progenitor cells have the capacity to balance self-renewal differentiation. Hematopoietic myeloid progenitors replenish more than 25 billion terminally differentiated neutrophils every day under homeostatic conditions can increase this output in response stress or infection. At what point along spectrum of maturation do lose for become irreversibly committed differentiation? Using a system conditional development that be toggled between differentiation, we interrogate determinants...
Triple-negative breast cancer (TNBC) contributes greatly to mortality of cancer, demanding new targetable options. We have shown that TNBC patients high
Background: Chinese hamster ovary (CHO) cells are extremely important host for recombinant DNA technology with their utility requiring optimization of growth. The ability to test conditions using in silico models growing CHO can help advance the bioreactor towards higher viable cell concentration and increased mAb production. Methods: A new kinetic model metabolism is presented, tested provided this publication. RNASeq data from was used guide selection major metabolic pathways that were...
Summary Cancer is the second leading cause of death globally, due primarily to metastatic dissemination and colonization distal sites. Recurrent genetic drivers metastasis are elusive, suggesting that, unlike stereotyped mutations promoting primary tumor development, may be variable. Here, we interrogate pathways governing through CRISPR/Cas9-based forward screening in a genetically defined colorectal adenocarcinoma organoid (tumoroid) model using e x vivo invasion screens orthotopic, for...
Abstract Colorectal cancer (CRC) is the 2nd-leading cause of cancer-related deaths worldwide and metastatic disease remains a major unmet clinical need. Nevertheless, molecular underpinnings spread colonization distant sites remain elusive. To systematically identify functional genes contributing to metastasis in physiologically relevant model CRC, we developed novel platform enabling robust, pooled CRISPR/Cas9 screening primary colonic organoids engineered with common CRC driver mutations...
<div>Abstract<p>Epigenetic programs are dysregulated in acute myeloid leukemia (AML) and help enforce an oncogenic state of differentiation arrest. To identify key epigenetic regulators AML cell fate, we performed a differentiation-focused CRISPR screen cells. This identified the histone acetyltransferase KAT6A as novel regulator that drives critical leukemogenic gene-expression programs. We show is initiator newly described transcriptional control module which KAT6A-catalyzed...
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
<div>Abstract<p>Epigenetic programs are dysregulated in acute myeloid leukemia (AML) and help enforce an oncogenic state of differentiation arrest. To identify key epigenetic regulators AML cell fate, we performed a differentiation-focused CRISPR screen cells. This identified the histone acetyltransferase KAT6A as novel regulator that drives critical leukemogenic gene-expression programs. We show is initiator newly described transcriptional control module which KAT6A-catalyzed...
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Summary Stem and progenitor cells have the capacity to balance self-renewal differentiation. Hematopoietic myeloid progenitors replenish more than 25 billion terminally differentiated neutrophils every day under homeostatic conditions can increase this output in response stress or infection. At what point along spectrum of maturation do lose for become irreversibly committed differentiation? Using a system conditional development that be toggled between differentiation, we interrogated...