A5001780088- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Toxoplasma gondii Research Studies
- CRISPR and Genetic Engineering
MRC Protein Phosphorylation and Ubiquitylation Unit
2019-2025
University of Dundee
2019-2025
University of Aberdeen
2014-2020
Nutrition Sciences (Belgium)
2013
Abstract Branched ubiquitin (Ub) chains constitute a sizable fraction of Ub polymers in human cells. Despite their abundance, our understanding branched function cell signaling has been stunted by the absence accessible methods and tools. Here we identify cellular branched-chain-specific binding proteins devise approaches to probe K48–K63-branched function. We establish method monitor cleavage linkages within complex unveil ATXN3 MINDY as debranching enzymes. engineer K48–K63 branch-specific...
Branched ubiquitin (Ub) chains make up a significant proportion of Ub polymers in human cells and are formed when two or more sites on single molecule modified with creating bifurcated architectures. Despite their abundance, we have poor understanding the cellular functions branched signals that stems from lack facile tools methods to study them. Here develop comprehensive pipeline define function, using K48-K63-branched as case study. We discover branch-specific binders and, by developing...
Branched ubiquitin chains are complex molecular structures in which two or more moieties attached to distinct lysine residues of a single molecule within polyubiquitin chain. These bifurcated architectures significantly expand the signalling capacity system. Although branched constitute substantial fraction cellular polyubiquitin, their biological functions largely remain enigmatic due nature and associated technical challenges studying them. Recent technological innovations have enabled...
tRNA gene copy number is a primary determinant of abundance and therefore the rate at which each delivers amino acids to ribosome during translation. Low-abundance tRNAs decode rare codons slowly, but it unclear genes might be subject tRNA-mediated regulation expression. Here, those mRNA targets were identified via global simulation In-silico translation rates compared for in both wild-type |${\rm{tRNA}}_{{\rm{CUG}}}^{{\rm{Gln}}}$|sup70-65 mutant, exhibits pseudohyphal growth phenotype 75%...
During protein synthesis, charged tRNAs deliver amino acids to translating ribosomes, and are then re-charged by tRNA synthetases (aaRS). In humans, mutant aaRS cause a diversity of neurological disorders, but their molecular aetiologies incompletely characterised. To understand system responses depletion, the yeast glutamine gene (GLN4) was transcriptionally regulated using doxycycline tet-off control. Depletion Gln4p inhibited growth, induced GCN4 acid starvation response, indicative...
ABSTRACT During protein synthesis, charged tRNAs deliver amino acids to translating ribosomes, and are then re-charged by tRNA synthetases (aaRS). In humans, mutant aaRS cause a diversity of neurological disorders, but their molecular aetiologies incompletely characterised. To understand system responses depletion, the yeast glutamine gene ( GLN4 ) was transcriptionally regulated using doxycycline tet-off control. Depletion Gln4p inhibited growth, induced GCN4 acid starvation response,...