Although plasma corticosterone is considered the main glucocorticoid involved in regulation of stress responses rodents, presence cortisol and whether its level can be used as an indicator for rodent activation remain to determined. In this study, effects estrous cycle stage, circadian rhythm, acute chronic (repeated or unpredictable) stressors various severities on dynamics correlation serum were examined mice. A strong (r = 0.6–0.85) between was observed throughout cycle, all day long,...

Abstract Previous studies indicate that stress damages oocytes with increased secretion of glucorticoids. However, although injection female mice cortisol decreased oocyte competence, exposure mouse directly to physiological or stress-induced concentrations glucorticoids did not affect maturation and embryo development. This study has explored the mechanisms by which glucocorticoids impair competence. Female were injected effects cortisol-injection on ovarian cell apoptosis Fas/FasL...

This study examined the role of CRH-induced ovarian cell apoptosis in restraint stress (RS)-induced impairment oocyte competence. Oocyte percentages apoptotic cumulus cells (CCs) did not differ between stressed and control mice before vitro maturation (IVM) but became significantly higher after IVM without serum, growth factor, hormone. The level Bcl2 mRNA decreased mural granulosa (MGCs) homogenates RS. Whereas estradiol, testosterone, IGF1 decreased, cortisol progesterone increased...

Abstract The mechanisms for the transition from non-surrounded nucleolus (NSN) to surrounded (SN) chromatin configuration during oocyte growth/maturation are unclear. By manipulating enzyme activities and measuring important molecules using small-follicle pig oocytes with a high proportion of NSN an extended germinal vesicle stage in vitro , this study has first time up-to-date established essential role intra-oocyte mitogen-activated protein kinase (MAPK) NSN-to-SN transition. Within 1–2 mm...

In spite of great efforts, the mechanisms for postovulatory oocyte aging are not fully understood. Although our previous work showed that FasL/Fas signaling facilitated aging, intra-oocyte pathways unknown. Furthermore, by which oxidative stress facilitates and causal relationship between Ca2+ rises caspase-3 activation cell cycle apoptosis during need detailed investigations. Our aim was to address these issues studying Fas/FasL accelerate aging. The results indicated sFasL released cumulus...

The mechanisms by which restraint stress impairs oocyte developmental potential are unclear. Factors causing differences between the of oocytes with surrounded nucleolus (SN) and that nonsurrounded (NSN) not fully characterized. Furthermore, relationship increased histone acetylation methylation competence in SN is particularly worth exploring using a system where configuration can be uncoupled (dissociated) from modifications. In this study, female mice were subjected to for 24 or 48 h 23...

It is known that oocytes and cumulus cells (CCs) are more resistant to apoptosis than other compartments of the antral follicle. However, although oocyte-secreted factors (OSFs) have been found be involved in suppressing bovine CC apoptosis, little about intracellular mechanisms by which OSFs render CCs apoptosis. Here, we show coculture with mouse or pig cumulus-denuded oocytes, culture recombinant growth differentiation factor-9 (GDF-9), oocyte-conditioned medium (POCM) significantly...

Transcription factor AP-2-alpha (Tfap2a) is an important sequence-specific DNA-binding protein that can regulate the transcription of multiple genes by collaborating with inducible viral and cellular enhancer elements. In this experiment, expression, localization, functions Tfap2a were investigated in mouse oocytes during maturation. Overexpression via microinjection Myc-Tfap2a mRNA into ooplasm, immunofluorescence, immunoblotting used to study role oocyte meiosis. According our results,...

In recent years, there have been considerable research advances on the antileukemic mechanisms of antidiabetic drug metformin. Our current studies shown that metformin suppresses cell viability, induces apoptosis, and downregulates mTORC1 signaling pathway both in Ph+ALL line primary blasts from Ph+ ALL patients, as well CML lines K562 (imatinib-sensitive) K562R (imatinib-resistance). We also activates ERK cells, SUP-B15, a side effect can be overcome by U0126 (MEK1/2 inhibitor) or imatinib....

Cisplatin (CDDP) is a commonly used drug for gallbladder cancer (GBC) chemotherapy. However, resistance to CDDP treatment results in relapse. Therefore, there need the development of more effective strategies overcome chemoresistance. Dual‑specificity phosphatase 1 (DUSP1) was reported be involved number chemotherapeutic agents and revealed highly expressed CDDP‑resistant GBC cells CDDP‑treated tumor types compared with normal or tissues present study. DUSP1 inhibit cytotoxicity two cell...

Abstract The epigenetic factors causing competence differences between SN (surrounded nucleolus) and NSN (non-surrounded oocytes, the significance for increased histone acetylation methylation in whether chromatin configuration or modification determines oocyte competence, are unclear. This study has addressed these issues by using ovary-holding (OH) stress models where was uncoupled from modifications developmental potential. Prepubertal mouse ovaries containing high percentages of oocytes...

Reported classifications of germinal vesicle (GV) chromatin configurations in pig oocytes were not done by uniform standards and they well correlated with oocyte competence. In this study, GV was classified into nonsurrounded nucleolus (NSN), surrounded (SN), partly NSN (pNSN) SN (pSN), prematurely condensed (cNSN), pNSN (cpNSN) pSN (cpSN), early diakinesis (ED) patterns. During vitro maturation 199 medium, from 1 to 2 mm follicles went consecutively through pNSN, pSN, cpSN, ED before...

Phosphatidylinositol‑3,4,5‑trisphosphate Rac exchanger 2 (PREX2), a regulator of the small guanosine triphosphatase Rac, demonstrates an inhibitory effect on activity phosphatase and tensin homolog (PTEN). Previously, PREX2 was implicated in regulation cell invasion hepatocellular carcinoma (HCC). However, exact role HCC proliferation migration, as well underlying mechanisms, remains unclear. In present study, reverse transcription‑quantitative polymerase chain reaction revealed that...

The developmental capacity of in vitro-matured (IVM) oocytes is markedly lower than that their vivo-matured (IVO) counterparts, suggesting the need for optimization IVM protocols different species. There are few studies on rat oocytes, and there even fewer attempts to improve ooplasmic maturation compared those reported other Furthermore, well known undergo spontaneous activation (SA) after leaving oviduct; however, whether have SA rates IVO can potentially be used nuclear transfer unknown....

It has been reported that microRNAs (miRs) have key roles in tumorigenesis via inhibition of their target genes. Dysregulation miR‑27b detected numerous types human cancer, including hepatocellular carcinoma (HCC); however, the detailed role HCC yet to be elucidated. Reverse transcription‑quantitative polymerase chain reaction and western blotting were performed examine mRNA protein expression levels. Transwell assay wound healing used determine cell invasion migration. Luciferase reporter...

To study the relationship between chromatin condensation, gene transcription and developmental competence during oocyte maturation to explore mechanisms by which meiotic arrest maintenance (MAM) sexual maturity improve competence, we examined effects of MAM with roscovitine or db-cAMP on potential NSN SN oocytes from prepubertal adult mice. improved global (prep-NSN) adult-SN while having no effect those prep-SN oocytes. facilitated neither development nor in any type either promoted...

Aconitum carmichaelii Debeaux is used as a traditional Chinese medicine with antiarrhythmic, antiinflammatory and other pharmacological functions. It widely cultivated in China. According to our survey, about 60% of A. Qingchuan, Sichuan, suffered from root rot, reducing yields by 30% the past five years. Symptomatic plants exhibited stunted growth, dark brown roots, reduced biomass, fewer hairs. The disease caused rot plant death 50% infected plants. In October 2019, ten symptomatic...

To explore the molecular mechanism of resistance to imatinib in K562 cells(K562-R) and anti-proliferative effect oridonin (OR), as well its imatinib-sensitive imatinib-resistant cells (K562-S K562-R cells).The expression p-Lyn K562-S were detected by Western blot. The OR was assayed MTT, morphological changes examined with microscope, cell apoptosis flow cytometry, expressions BCL-2 Akt/mTOR signaling pathway blot.The over-expression detcected cells, inhibited proliferation value IC50...