A5005037429- Malaria Research and Control
- Eosinophilic Esophagitis
- Complement system in diseases
- HIV Research and Treatment
- IL-33, ST2, and ILC Pathways
- Food Allergy and Anaphylaxis Research
- Biochemical and Molecular Research
- ATP Synthase and ATPases Research
- RNA regulation and disease
- Signaling Pathways in Disease
- Trypanosoma species research and implications
- Parasites and Host Interactions
- Spectroscopy and Laser Applications
- Spectroscopy and Quantum Chemical Studies
- Enzyme Structure and Function
- Calpain Protease Function and Regulation
- Enzyme function and inhibition
- Proteoglycans and glycosaminoglycans research
- Blood Coagulation and Thrombosis Mechanisms
- RNA modifications and cancer
- vaccines and immunoinformatics approaches
- Pneumocystis jirovecii pneumonia detection and treatment
- Advanced Steganography and Watermarking Techniques
- Porphyrin and Phthalocyanine Chemistry
- Viral gastroenteritis research and epidemiology
University of Oxford
2019-2024
National Institute of Technology Hamirpur
2020
International Centre for Genetic Engineering and Biotechnology
2011-2018
All India Institute of Medical Sciences
2015
Department of Biotechnology
2015
Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya
2013
Highlights•Human PfRH5 vaccination induces cross-reactive neutralizing antimalarial antibodies•Neutralizing human antibodies bind epitopes close to the basigin binding site•Some non-neutralizing potentiate those several malaria proteins•Potentiating slow erythrocyte invasion by a new epitope on PfRH5SummaryThe Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) is leading target for next-generation vaccines against disease-causing blood-stage of malaria. However, little...
Abstract Understanding mechanisms of antibody synergy is important for vaccine design and cocktail development. Examples between antibodies are well-documented, but the underlying these relationships often remain poorly understood. The leading blood-stage malaria candidate, CyRPA, essential invasion Plasmodium falciparum into human erythrocytes. Here we present a panel anti-CyRPA monoclonal that strongly inhibit parasite growth in vitro assays. Structural studies show growth-inhibitory bind...
ABSTRACT HpARI is an immunomodulatory protein secreted by the intestinal nematode Heligmosomoides polygyrus bakeri , which binds and blocks IL-33. Here, we find that H. genome contains three family members these have different effects on IL-33-dependent responses in vitro vivo with HpARI1+2 suppressing HpARI3 amplifying responses. All HpARIs sub-nanomolar affinity for mouse IL-33; however, does not block IL-33-ST2 interactions. Instead, stabilizes IL-33, increasing half-life of cytokine to...
The IL-33-ST2 pathway is an important initiator of type 2 immune responses. We previously characterised the HpARI protein secreted by model intestinal nematode Heligmosomoides polygyrus, which binds and blocks IL-33. Here, we identify H. polygyrus Binds Alarmin Receptor Inhibits (HpBARI) HpBARI_Hom2, both consist complement control (CCP) domains, similarly to immunomodulatory Hp-TGM proteins. HpBARI murine ST2, inhibiting cell surface detection preventing interactions, IL-33 responses in...
Abstract The symptoms of malaria occur during the blood stage infection, when parasites invade and replicate within human erythrocytes. PfPCRCR complex 1 , containing PfRH5 (refs. 2,3 ), PfCyRPA, PfRIPR, PfCSS PfPTRAMP, is essential for erythrocyte invasion by deadliest parasite, Plasmodium falciparum . Invasion can be prevented antibodies 3–6 or nanobodies against each these conserved proteins, making them leading blood-stage vaccine candidates. However, little known about how functions...
Basigin is an essential host receptor for invasion of Plasmodium falciparum into human erythrocytes, interacting with parasite surface protein PfRH5. PfRH5 a leading blood-stage malaria vaccine candidate and target growth-inhibitory antibodies. Here, we show that erythrocyte basigin exclusively found in one two macromolecular complexes, bound either to plasma membrane Ca 2+ -ATPase 1/4 (PMCA1/4) or monocarboxylate transporter 1 (MCT1). binds each these complexes higher affinity than isolated...
IL-33 plays a significant role in inflammation, allergy, and host defence against parasitic helminths. The model gastrointestinal nematode Heligmosomoides polygyrus bakeri secretes the Alarmin Release Inhibitor HpARI2, an effector protein that suppresses protective immune responses asthma its by inhibiting signalling. Here we reveal structure of HpARI2 bound to mouse IL-33. contains three CCP-like domains, show it contacts primarily through second third these. A large loop which emerges from...
Accuracy of aminoacylation is dependent on maintaining fidelity during attachment amino acids to cognate tRNAs. Cis- and trans-editing protein factors impose quality control translation 8 36 Plasmodium falciparum aminoacyl-tRNA synthetase (aaRS) assemblies contain canonical putative editing modules. Based expression localization profiles these aaRSs, we propose an asymmetric distribution between the parasite cytoplasm its apicoplast editing-domain containing aaRSs. We also show that single...
India is world's largest democracy and the essence of any lies in fact that people choose their own representatives. But present era, fair election process facing a lot problems like booth capturing, rigging, fake voting, tampering with Electronic Voting Machines (EVMs) etc. Being responsible engineers, it's our duty to do something curb this menace. In commonly used EVMs, voting takes place electronically eliminates use ballot paper cast votes elections as it very time consuming errors...
Abstract Interleukin 33 (IL-33) plays a significant role in inflammation, allergy, and host defence against parasitic helminths. The model gastrointestinal nematode Heligmosomoides polygyrus bakeri (Hp) secretes the Alarmin Release Inhibitor (HpARI), potent effector protein which suppresses protective immune responses asthma its by inhibiting IL-33 signalling. Here we reveal structure of HpARI bound to mouse IL-33. contains three CCP-like domains, show that it contacts primarily through...
Background The monkey malaria parasite Plasmodium knowlesi also infect humans. There is a lack of information on the molecular mechanisms that take place between this simian and its heterologous human host erythrocytes leading to zoonotic disease. Therefore, we investigated here binding ability P. tryptophan-rich antigens (PkTRAgs) sharing erythrocyte receptors them as well with other commonly occurring parasites. Methods Six PkTRAgs were cloned expressed in E.coli mammalian CHO-K1 cell...
The symptoms of malaria occur during the blood stage infection, when parasites invade and replicate within human erythrocytes. five-component PfPCRCR complex, containing PfRH5, PfCyRPA, PfRIPR, PfCSS PfPTRAMP, is essential for erythrocyte invasion by deadliest parasite, Plasmodium falciparum. Invasion can be prevented antibodies or nanobodies against each these five conserved proteins, making them leading vaccine candidates. However, little known about molecular mechanism which functions...
Abstract The parasitic nematode Heligmosomoides polygyrus bakeri secretes the HpARI family, which bind to IL-33, either suppressing (HpARI1 and HpARI2) or enhancing (HpARI3) responses cytokine. We previously showed that HpARI2 also bound DNA via its first Complement Control Protein (CCP1) domain. Here, we find HpARI1 can DNA, while HpARI3 cannot. Through production of HpARI2/HpARI3 CCP1 domain-swapped chimeras, DNA-binding ability be transferred, correlates with in vivo half-life...
The parasitic nematode Heligmosomoides polygyrus bakeri secretes the HpARI family, which bind to IL-33, either suppressing (HpARI1 and HpARI2) or enhancing (HpARI3) responses cytokine. We previously showed that HpARI2 also bound DNA via its first Complement Control Protein (CCP1) domain. Here, we find HpARI1 can DNA, while HpARI3 cannot. Through production of HpARI2/HpARI3 CCP1 domain-swapped chimeras, DNA-binding ability be transferred, correlates with in vivo half-life administered...
The parasitic nematode Heligmosomoides polygyrus bakeri secretes the HpARI family, which bind to IL-33, either suppressing (HpARI1 and HpARI2) or enhancing (HpARI3) responses cytokine. We previously showed that HpARI2 also bound DNA via its first complement control protein (CCP1) domain. Here, we find HpARI1 can DNA, while HpARI3 cannot. Through production of HpARI2/HpARI3 CCP1 domain-swapped chimeras, DNA-binding ability be transferred, correlates with in vivo half-life administered...
Abstract Basigin is an essential host receptor for invasion of Plasmodium falciparum into human erythrocytes, interacting with parasite surface protein PfRH5. PfRH5 a leading blood-stage malaria vaccine candidate and target growth-inhibitory antibodies. However, basigin not alone on the erythrocyte surface. Instead, we show that it exclusively found in one two macromolecular complexes, bound predominantly to either plasma membrane Ca 2+ -ATPase 1/4, PMCA1/4, or monocarboxylate transporter 1,...
Inorganic pyrophosphatases (PPase) participate in energy cycling and they are essential for growth survival of organisms. Here we report extensive structural functional characterization soluble PPases from the human parasites Plasmodium falciparum (PfPPase) Toxoplasma gondii (TgPPase). Our results show that PfPPase is a cytosolic enzyme whose gene expression upregulated during parasite asexual stages. Cambialistic actively hydrolyzes linear short chain polyphosphates like PPi, polyP3 ATP...
The parasitic nematode Heligmosomoides polygyrus bakeri secretes the HpARI family, which bind to IL-33, either suppressing (HpARI1 and HpARI2) or enhancing (HpARI3) responses cytokine. We previously showed that HpARI2 also bound DNA via its first complement control protein (CCP1) domain. Here, we find HpARI1 can DNA, while HpARI3 cannot. Through production of HpARI2/HpARI3 CCP1 domain-swapped chimeras, DNA-binding ability be transferred, correlates with in vivo half-life administered...
The parasitic nematode Heligmosomoides polygyrus bakeri secretes the HpARI family, which bind to IL-33, either suppressing (HpARI1 and HpARI2) or enhancing (HpARI3) responses cytokine. We previously showed that HpARI2 also bound DNA via its first Complement Control Protein (CCP1) domain. Here, we find HpARI1 can DNA, while HpARI3 cannot. Through production of HpARI2/HpARI3 CCP1 domain-swapped chimeras, DNA-binding ability be transferred, correlates with in vivo half-life administered...