A5111492101- Protein Kinase Regulation and GTPase Signaling
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Protein Structure and Dynamics
- Glycosylation and Glycoproteins Research
- Immune Response and Inflammation
- Plant Taxonomy and Phylogenetics
- Enzyme Structure and Function
- Oral microbiology and periodontitis research
- Topic Modeling
- Neurofibromatosis and Schwannoma Cases
- Receptor Mechanisms and Signaling
- Skin and Cellular Biology Research
- RNA Research and Splicing
- Mediterranean and Iberian flora and fauna
- Biotin and Related Studies
- Artificial Intelligence in Healthcare
- Monoclonal and Polyclonal Antibodies Research
- Advanced Proteomics Techniques and Applications
- Ubiquitin and proteasome pathways
- Cell Adhesion Molecules Research
- Machine Learning in Healthcare
- Caveolin-1 and cellular processes
- Cellular transport and secretion
Institute of Molecular and Cell Biology
1998-2005
The p21-activated kinase PAK is targeted to focal complexes (FCs) through interactions with the SH3 domains of PAK-interacting exchange factor PIX and Nck. a Rac GTP that also binds G-protein-coupled receptor kinase-interacting protein known as GIT1. Overexpression GIT1 in fibroblasts or epithelial cells causes loss paxillin from FCs stimulates cell motility. This due direct interaction C-terminal 125-residue domain paxillin, under regulation PIX. In its activated state, can promote FC...
ABSTRACTαPAK in a constitutively active form can exert morphological effects (E. Manser, H.-Y. Huang, T.-H. Loo, X.-Q. Chen, J.-M. Dong, T. Leung, and L. Lim, Mol. Cell. Biol. 17:1129–1143, 1997) resembling those of Cdc42G12V. PAK family kinases, conserved from yeasts to humans, are directly activated by Cdc42 or Rac1 through interaction with N-terminal motif (corresponding residues 71 137 αPAK). αPAK mutants substitutions this that resulted severely reduced binding be recruited normally...
The kinase PAK binds tightly to the SH3 domain of its partner PIX via a central proline-rich sequence.A different N-terminal sequence allows ␣PAK bind an adaptor Nck.The Nck SH3[2] interacts equally with 18-mer PAK-derived peptide and full-length ␣PAK.Detailed analysis this binding by saturation substitution related targets be accurately identified from characteristics alone.All proteins, including PAK, NIK, synaptojanin, PRK2, WIP, possess motif PXXPXRXXS; in case serine phosphorylation at...
PIX is a Rho-family guanine nucleotide exchange factor that binds PAK. We previously described two isoforms of differ in their N termini. Here, we report the identification new splice variant βPIX, designated β2PIX, dominant species brain and lacks region ∼120 residues with predicted coiled-coil structure at C terminus β1PIX. Instead, β2PIX contains serine-rich terminus. To determine whether these variants cellular function, studied effect expressing proteins HeLa cells. found plays key role...
AbstractA variety of techniques can be used to find protein partners, including immunoprecipitation, affinity chromatography, blot overlays, and yeast twohybrid screening. Of these, the overlay protocol sodium dodecyl sulfate (SDS)-acrylamide-gel-separated proteins provides perhaps most direct assessment target binding, since one simultaneously screen multiple cell types or tissue extracts, infer size-and in some cases relative affinity-of binding proteins. Combined use with other such as...