A5114406787- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Computational Drug Discovery Methods
- Advanced Biosensing Techniques and Applications
- bioluminescence and chemiluminescence research
- Animal health and immunology
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- thermodynamics and calorimetric analyses
- RNA modifications and cancer
- Biosensors and Analytical Detection
- Pharmaceutical Practices and Patient Outcomes
- Animal Virus Infections Studies
- Cancer-related molecular mechanisms research
- Nanowire Synthesis and Applications
- Lung Cancer Treatments and Mutations
- Wnt/β-catenin signaling in development and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Virus-based gene therapy research
- Barrier Structure and Function Studies
- Health Systems, Economic Evaluations, Quality of Life
Hamad bin Khalifa University
2022-2024
Qatar Foundation
2022-2024
The main protease, Mpro, is critical for SARS-CoV-2 replication and an appealing target designing anti-SARS-CoV-2 agents. Therefore, there a demand the development of improved sensors to monitor its activity. Here, we report pair genetically encoded, bioluminescence resonance energy transfer (BRET)-based detecting Mpro proteolytic activity in live cells as well vitro. were generated by sandwiching peptides containing N-terminal autocleavage sites, either AVLQSGFR (short) or KTSAVLQSGFRKME...
NanoLuc (NLuc) luciferase has found extensive application in designing a range of biological assays, including gene expression analysis, protein-protein interaction, and protein conformational changes due to its enhanced brightness small size. However, questions related mechanism interaction with the substrate, furimazine, as well bioluminescence activity remain elusive. Here, we combined molecular dynamics (MD) simulation mutational analysis show that R162A mutation results decreased but...
Anti-COVID19 drugs, such as nirmatrelvir, have been developed targeting the SARS-CoV-2 main protease, M
Abstract The SARS-CoV-2 main protease, M pro , is critical for its replication and an appealing target designing anti-SARS-CoV-2 agents. In this regard, a number of assays have been developed based on cleavage sequence preferences to monitor activity. These include the usage Fluorescence Resonance Energy Transfer (FRET)-based substrates in vitro FlipGFP reporter, one which fluoresces after -mediated cleavage, live cells. Here, we engineered pair genetically encoded, Bioluminescence...
Summary While SARS-CoV-2 M pro and PL proteases are known to cleave polyproteins pp1a pp1ab at multiple sites, these have not been comprehensively characterized in living cells. Here we engineered a two-color Bioluminescence Resonance Energy Transfer (BRET)-based, dual protease (DuProSense) biosensor platform relying on proximity-dependent energy transfer from luciferase donor two spectrally separated fluorescent protein acceptors enabling simultaneous monitoring of processing cleavage sites...
Abstract NanoLuc (NLuc) luciferase has found extensive application in designing a range of biological assays including gene expression analysis, protein-protein interaction and protein conformational changes due to its enhanced brightness small size. However, questions related mechanism with the substrate, furimazine, as well bioluminescence activity remains elusive. Here, we combined molecular dynamics (MD) simulation mutational analysis show that R162A mutation results decreased but stable...