A5039789764- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Bioeconomy and Sustainability Development
- Advanced Biosensing Techniques and Applications
- Escherichia coli research studies
- Influenza Virus Research Studies
- Glycosylation and Glycoproteins Research
- Animal Genetics and Reproduction
- Infant Nutrition and Health
- Viral Infectious Diseases and Gene Expression in Insects
- vaccines and immunoinformatics approaches
Osaka University
2020-2024
Species A rotaviruses (RVs) are a leading cause of severe acute gastroenteritis in infants and children younger than 5 years. Currently available RV vaccines were adapted from wild-type strains by serial passage cultured cells or reassortment between human animal strains. These traditional methods require large-scale screening genotyping to obtain vaccine candidates. Reverse genetics is tractable, rapid, reproducible approach generating recombinant candidates carrying any VP4 VP7 genes that...
Rotaviruses (RVs) are an important cause of acute gastroenteritis in young children. Recently, versatile plasmid-based reverse genetics systems were developed for several human RV genotypes; however, these have not been all commonly circulating genotypes. In this study, we established a system G2P[4] strain HN126. Nucleotide sequence analysis, including that the terminal ends viral double-stranded RNA genome, revealed HN126 possessed DS-1-like genotype constellation. Eleven plasmids, each...
The entry of SARS-CoV-2 into host cells is mediated by the interaction between spike receptor-binding domain (RBD) and angiotensin-converting enzyme 2 (ACE2). Certain human antibodies, which target N-terminal (NTD) at a distant epitope from cell binding surface, have been found to augment ACE2 enhance infection. Notably, these antibodies exert their effect independently antibody fragment crystallizable (Fc) region, distinguishing mode action previously described antibody-dependent...
Abstract Antibody dependent enhancement (ADE) is a serious concern in vaccine development. The canonical ADE pathways are on the fragment crystallizable (Fc) region of antibody. In SARS-CoV-2 several antibodies have been discovered that inflict vitro. These target N-terminal domain (NTD) spike protein. We previously proposed these NTD-targeting infection-enhancing (NIEAs) cross-link neighboring proteins via their NTDs, and this results decoupling between NTD receptor binding (RBD),...