A5052573450- DNA Repair Mechanisms
- Metabolism and Genetic Disorders
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Cancer Research and Treatments
- Antibiotic Resistance in Bacteria
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
The Francis Crick Institute
2024
Newly copied sister chromatids are tethered together by the cohesin complex, but how chromatid cohesion coordinates with DNA replication is poorly understood. Prevailing models suggest that complexes, bound to before replication, remain behind advancing fork keep together. By visualizing single forks colliding preloaded we find replisome instead pushes where a converging met. Whereas replisomes removed during termination, remains on nascent and provides cohesion. Additionally, show CMG...
DNA-protein crosslinks (DPCs) pose significant barriers to DNA replication and genome integrity. Previous studies have established that bypass of DPCs by the eukaryotic replicative helicase, CMG complex, is critical for DPC repair. We investigated mechanism leading-strand using purified components. Our results reveal can in presence downstream single-stranded DNA, without aid other replisome factors. a slow process, its efficiency depends on size structure protein barrier. does not require...