A5102932105- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Protein Degradation and Inhibitors
- Photosynthetic Processes and Mechanisms
- Studies on Chitinases and Chitosanases
Tsinghua University
2023-2024
Center for Life Sciences
2023-2024
Shanghai Jiao Tong University
2024
King Center
2023
The chemical synthesis of histones with homogeneous modifications is a powerful approach for quantitatively deciphering the functional crosstalk between different post-translational (PTMs). In this study, we developed an expedient site-specific (poly)ubiquitylation strategy (CAEPL, Cysteine Aminoethylation coupled Enzymatic Protein Ligation), which integrates Cys-aminoethylation reaction process ubiquitin-activating enzyme UBA1-assisted native ligation. Using strategy, successfully prepared...
Abstract Recent research reported an effector protein SAP05 secreted by phytoplasma could hijack hostage Rpn10 subunit of proteasome and target degradation GATA or SPL transcription factors (TFs) without ubiquitin. As a result, plant phenotypes will be reprogrammed to state suitable for amplification insect vector propagation which present prolonged growth cycles, witches’ broom-like proliferations leaves sterile shoots. We are curious about how bypassing the ubiquitin pathway if there is...
Besides traditional ubiquitin-dependent proteasome degradation, thousands of eukaryotic proteins more than previously appreciated could undergo ubiquitin-independent proteasomal degradation (UbInPD). A pathogen-encoded effector protein SAP05 secreted by phytoplasma, hijack hostage Rpn10 subunit derived from Arabidopsis thaliana and target the GATA BINDING FACTOR (GATA) or SQUAMOSA-PROMOTER PROTEIN-LIKE (SPL) transcription factors without ubiquitin additional shuttle factors. To explain how...