A5010786451- Epigenetics and DNA Methylation
- Pancreatic and Hepatic Oncology Research
- RNA modifications and cancer
- Tissue Engineering and Regenerative Medicine
- Sunflower and Safflower Cultivation
- Liver Disease Diagnosis and Treatment
- Genetic diversity and population structure
- Cancer Cells and Metastasis
- Plant and animal studies
- Plant-Microbe Interactions and Immunity
- Potato Plant Research
- Cancer-related gene regulation
- Drug Transport and Resistance Mechanisms
- Microtubule and mitosis dynamics
- Axon Guidance and Neuronal Signaling
- Plant responses to water stress
- Genetics and Neurodevelopmental Disorders
- Microbial Metabolites in Food Biotechnology
- Adenosine and Purinergic Signaling
- Genetic and Environmental Crop Studies
- Plant Stress Responses and Tolerance
- Microbial Community Ecology and Physiology
- interferon and immune responses
- Hippo pathway signaling and YAP/TAZ
- Cytokine Signaling Pathways and Interactions
Salk Institute for Biological Studies
2023-2025
University of British Columbia
2019-2021
The metabolic landscape of cancer greatly influences antitumor immunity, yet it remains unclear how organ-specific metabolites in the tumor microenvironment influence immunosurveillance. We found that accumulation primary conjugated and secondary bile acids (BAs) are features human hepatocellular carcinoma experimental liver models. Inhibiting BA synthesis hepatocytes through deletion BA-conjugating enzyme acid–CoA:amino acid N -acyltransferase (BAAT) enhanced tumor-specific T cell...
Species often include multiple ecotypes that are adapted to different environments. But how do arise, and their distinctive combinations of adaptive alleles maintained despite hybridization with non-adapted populations? Re-sequencing 1506 wild sunflowers from three species identified 37 large (1-100 Mbp), non-recombining haplotype blocks associated numerous ecologically relevant traits, soil climate characteristics. Limited recombination in these regions keeps together, we find they...
The use of patient-derived organoids (PDOs) to characterize therapeutic sensitivity and resistance is a promising precision medicine approach, its potential inform clinical decisions now being tested in several large multiinstitutional trials. PDOs are cultivated the extracellular matrix from basement membrane extracts (BMEs) that most commonly acquired commercially. Each site utilizes distinct BME lots may be restricted due availability commercial sources. However, effect different sources...
Summary All organisms experience stress as an inevitable part of life, from single-celled microorganisms to complex multicellular beings. The ability recover is a fundamental trait that determines the overall resilience organism, yet recovery understudied. To begin unraveling process we studies drought in Arabidopsis thaliana . We performed fine-scale time series bulk RNA sequencing starting 15 minutes after rehydration following moderate drought. reveal rapid involving activation thousands...
Abstract Mathematical models of biochemical reaction networks are an important and emerging tool for the study cell signaling involved in disease processes. One promising potential application such mathematical is how disease-causing mutations promote phenotype that contributes to disease. It commonly assumed one must have a thorough characterization network readily available modeling be useful, but we hypothesized could useful when there incomplete knowledge it discovery opens new areas...
Abstract Motivation Whole-genome bisulfite sequencing is a powerful tool for analyzing chromatin methylation genome-wide, but analysis of whole-genome data slow, inflexible, and often inaccurate. Results We developed PCBS, computationally efficient R package Whole Genome Bisulfite Sequencing that demonstrates remarkable accuracy flexibility compared to current tools. PCBS identifies differentially methylated loci, regions, offers novel functionality allows more targeted analyses. uses...
The use of patient-derived organoids (PDOs) to characterize therapeutic sensitivity and resistance (pharmacotyping) is a promising precision medicine approach. potential this approach inform clinical decisions now being tested in several large multi-institutional trials. PDOs are cultivated extracellular matrix from basement membrane extracts (BMEs) that most commonly acquired commercially. Each site utilizes distinct BME lots may be restricted due the availability commercial sources....
<title>Abstract</title> Age is a major risk factor for liver cancer, as the case most adult human cancers. However, underlying mechanisms are not well defined. A better understanding of role aging in and other cancers can facilitate approaches assessment, early detection prevention. We hypothesize that age-driven changes render aged more sensitive to oncogenic stress hence tumorigenesis. To investigate how with age, we documented immune profile, transcriptome epigenome healthy livers from...
Abstract Motivation Whole-genome bisulfite sequencing is a powerful tool for analyzing chromatin methylation genome-wide, but analysis of whole-genome data hampered by slow, inaccurate, and inflexible pipelines. Results We developed PCBS, computationally efficient R package Whole Genome Bisulfite Sequencing that demonstrates remarkable accuracy flexibility compared to current tools. PCBS identifies differentially methylated loci regions offers novel functionality allows more targeted...
Abstract Age is a major risk factor for liver cancer, as the case most adult human cancers. However, underlying mechanisms are not well defined. A better understanding of role aging in and other cancers can facilitate approaches assessment, early detection prevention. We hypothesize that age-driven changes render aged more sensitive to oncogenic stress hence tumorigenesis. To investigate how with age, we documented immune profile, transcriptome epigenome healthy livers from both young mice,...
Abstract Background and Aims Chronic pancreatitis (CP) affects more than 200,000 Americans 1 million individuals world wide, but treatment generally focuses on supportive care like pain management. 28-80% of CP cases are idiopathic 10-15% hereditary with mutations in PRSS1, SPINK1, CFTR, other genes. Developing human models to understand the drivers disease targeting key players this will provide novel therapeutic approaches for these patients who have limited options. Methods Utilizing...