A5086808543- Tissue Engineering and Regenerative Medicine
- Photosynthetic Processes and Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Cellular transport and secretion
- Circular RNAs in diseases
- Genetics, Aging, and Longevity in Model Organisms
- Axon Guidance and Neuronal Signaling
- MicroRNA in disease regulation
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Bone Tissue Engineering Materials
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Retinoids in leukemia and cellular processes
- RNA regulation and disease
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Neuroblastoma Research and Treatments
- Hedgehog Signaling Pathway Studies
- Microtubule and mitosis dynamics
- Stress Responses and Cortisol
- Neurobiology and Insect Physiology Research
- Mitochondrial Function and Pathology
Duke-NUS Medical School
2011-2016
Stanford University
2006-2011
Abstract The vitamin A metabolite, all-trans retinoic acid (atRA), is required for embryonic development. atRA binds to the nuclear receptors and regulates transcription of specific target genes. In order identify atRA-induced genes that play a role in neural development, subtractive library was created from SH-SY5Y neuroblastoma cells, human cell line exhibits changes adhesion neurite outgrowth after exposure acid. We report here identification 14 are rapidly induced by (retinoic...
MicroRNAs (miRNAs) are short non-coding RNAs that emerging as important post-transcriptional regulators of neuronal and synaptic development. The precise impact miRNAs on presynaptic function neurotransmission remains, however, poorly understood. Here, we identify miR-27b—an abundant miRNA implicated in neurological disorders—as a global regulator the transcriptome. miR-27b influences expression three quarters genes associated with cortical neurons. Contrary to expectation, large majority...
Presynaptic assembly involves the specialization of a patch axonal membrane into complex structure that supports synaptic vesicle exocytosis and neurotransmitter release. In mammalian neurons, presynaptic is widely studied in co-culture assay, where synaptogenic cue expressed at surface heterologous cell induces differentiation contacting axon. This assay has led to discovery numerous proteins, but not been used probe neuronal mechanisms regulating induction. The identification regulatory...
MicroRNAs (miRNAs), short non-coding RNAs that repress gene expression [1], have recently emerged as important regulators of synaptic transmission and plasticity in the central nervous system [2]. This suggests an role this silencing mechanism brain function both health disease states [3]. We previously reported a novel disease-associated miR-27b promoting presynaptic assembly [4]. To further understand miRNA brain, we knocked down mouse cortical neurons studied impact perturbation on...